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Anti cav2

Manufactured by BD
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Anti-Cav2 is a laboratory equipment product designed to detect and quantify the expression of the Cav2 protein. It functions as a tool for researchers to study the role of Cav2 in various biological processes.

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Lab products found in correlation

Anti lamin b1, by Abcam (2 mentions) Anti lamin a, by Merck Group (2 mentions) Anti pom121, by Thermo Fisher Scientific (2 mentions) Anti nup210, by Fortis Life Sciences (2 mentions) Anti nup88, by BD (2 mentions) Anti nup107, by Cell Signaling Technology (2 mentions) Anti nup98, by Cell Signaling Technology (2 mentions) Anti nup93 f2, by Santa Cruz Biotechnology (2 mentions) Anti cav1, by Cell Signaling Technology (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Sp8 confocal microscope, by Leica (2 mentions) Mab414, by Fortrea (2 mentions) Vectashield, by Vector Laboratories (2 mentions)

2 protocols using anti cav2

1

Immunofluorescence Staining of Primary Liver Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary liver cells fixed in 4 methanol-free % PFA for 5 minutes were blocked and stained in IF buffer (1x PBS, 10 mg/ml BSA, 0.02% SDS, 0.1% Triton X-100). Primary and secondary antibodies was incubated for 1 hour at RT or overnight at 4°C with IF buffer washes between and after incubations. Cells were labeled with Hoechst 33342 (Life Technologies) for 5 minutes before mounting using VECTASHIELD (Vector Laboratories). Antibodies used: mAb414 (Covance or BioLegend); anti-Nup210 (Bethyl Laboratories, Inc.); anti-Pom121 (ThermoFisher Scientific); anti-Nup88 (22, BD Transduction Laboratories); anti-Nup107 (a kind gift from Martin Hetzer9 (link), The Salk Institute, La Jolla CA, USA); anti-Nup98 (39A3, Cell Signaling Technology); anti-Nup93 (F2, Santa Cruz BioTechnology); anti-Lamin A (Sigma Aldrich); anti-Lamin B1 (Abcam); anti-Cav1 (Cell Signaling Technology), and anti-Cav2 (65, BD Biosciences). Images were taken using a Leica SP8 confocal microscope and analyzed using the Leica Application Suite X software v3.1.5.16308, ImageJ v2.0.0-rc-54/1.51h (NIH), and Adobe Photoshop CS5.1 v12.1 x64.
Borlido J., Sakuma S., Raices M., Carrette F., Tinoco R., Bradley L.M, & D’Angelo M.A. (2018). Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nature immunology, 19(6), 594-605.
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2

Immunofluorescence Staining of Primary Liver Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary liver cells fixed in 4 methanol-free % PFA for 5 minutes were blocked and stained in IF buffer (1x PBS, 10 mg/ml BSA, 0.02% SDS, 0.1% Triton X-100). Primary and secondary antibodies was incubated for 1 hour at RT or overnight at 4°C with IF buffer washes between and after incubations. Cells were labeled with Hoechst 33342 (Life Technologies) for 5 minutes before mounting using VECTASHIELD (Vector Laboratories). Antibodies used: mAb414 (Covance or BioLegend); anti-Nup210 (Bethyl Laboratories, Inc.); anti-Pom121 (ThermoFisher Scientific); anti-Nup88 (22, BD Transduction Laboratories); anti-Nup107 (a kind gift from Martin Hetzer9 (link), The Salk Institute, La Jolla CA, USA); anti-Nup98 (39A3, Cell Signaling Technology); anti-Nup93 (F2, Santa Cruz BioTechnology); anti-Lamin A (Sigma Aldrich); anti-Lamin B1 (Abcam); anti-Cav1 (Cell Signaling Technology), and anti-Cav2 (65, BD Biosciences). Images were taken using a Leica SP8 confocal microscope and analyzed using the Leica Application Suite X software v3.1.5.16308, ImageJ v2.0.0-rc-54/1.51h (NIH), and Adobe Photoshop CS5.1 v12.1 x64.
Borlido J., Sakuma S., Raices M., Carrette F., Tinoco R., Bradley L.M, & D’Angelo M.A. (2018). Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nature immunology, 19(6), 594-605.
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