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Anti actin α smooth muscle fitc

Manufactured by Merck Group

Anti-actin α-smooth muscle- FITC is a fluorescently-labeled antibody that binds to α-smooth muscle actin, a contractile protein found in smooth muscle cells. It can be used for the identification and visualization of smooth muscle cells in various tissues and cell cultures.

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2 protocols using anti actin α smooth muscle fitc

1

Immunofluorescence Staining of Endothelial Markers

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The following primary antibodies were used at the indicated concentrations: MYC-Tag for COUP-TF2OE (Cell Signaling Technology, Inc., 2278S, 1:300), VE-Cadherin (BD Pharmingen, 550548, 1:125), VEGFR2 (R&D Systems, AF644, 1:125), Cx40 (Alpha Diagnostic International, CX40A, 1:300), ERG (Abcam, ab92513, 1:500), CXCR4 (BD Pharmingen, 551852, 1:125), GFP (Abcam, ab13970, 1:500), VWF (Abcam, ab6994, 1:500), CLDN11 (Abcam, ab53041, 1:1000), SOX17 (R&D Systems, AF1924, 1:500), anti-actin α-smooth muscle- FITC (Sigma, F3777, 1:200), VEGFR3 (R&D Systems, AF743, 1:125), DACH1 (Proteintech, 10914-1-AP, 1:500), JAG1 (R&D Systems, AF599, 1:125).
All secondary antibodies were Alexa Fluor conjugates (488, 555, 633, 635, 594, 647, Life Technologies, 1:125 or 1:250). DAPI (1mg/ml) was used at 1:500.
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2

Immunofluorescence Staining of Endothelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used at the indicated concentrations: MYC-Tag for COUP-TF2OE (Cell Signaling Technology, Inc., 2278S, 1:300), VE-Cadherin (BD Pharmingen, 550548, 1:125), VEGFR2 (R&D Systems, AF644, 1:125), Cx40 (Alpha Diagnostic International, CX40A, 1:300), ERG (Abcam, ab92513, 1:500), CXCR4 (BD Pharmingen, 551852, 1:125), GFP (Abcam, ab13970, 1:500), VWF (Abcam, ab6994, 1:500), CLDN11 (Abcam, ab53041, 1:1000), SOX17 (R&D Systems, AF1924, 1:500), anti-actin α-smooth muscle- FITC (Sigma, F3777, 1:200), VEGFR3 (R&D Systems, AF743, 1:125), DACH1 (Proteintech, 10914-1-AP, 1:500), JAG1 (R&D Systems, AF599, 1:125).
All secondary antibodies were Alexa Fluor conjugates (488, 555, 633, 635, 594, 647, Life Technologies, 1:125 or 1:250). DAPI (1mg/ml) was used at 1:500.
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