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Platimum quantitative pcr supermix udg

Manufactured by Thermo Fisher Scientific
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The Platimum® Quantitative PCR SuperMix-UDG is a pre-optimized and ready-to-use solution designed for real-time quantitative PCR (qPCR) applications. It contains all the necessary components for PCR amplification, including a thermostable DNA polymerase, dNTPs, and buffers. The mix also includes uracil-DNA glycosylase (UDG) for carryover prevention, which helps to eliminate the risk of cross-contamination between samples.

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Lab products found in correlation

Omniscript rt kit, by Qiagen (3 mentions) Rt2 sybr green qpcr mastermix, by Qiagen (2 mentions) Mastercycler ep realplex2 link system, by Eppendorf (2 mentions) Ssoadvanced universal sybr green supermix, by Bio-Rad (1 mentions) Mastercycler ep realplex2 system, by Eppendorf (1 mentions)

Close spelling variants of this product

PCR SuperMix (56 citations)

3 protocols using platimum quantitative pcr supermix udg

1

Quantitative RT-PCR Analysis of Gene Expression

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Total RNA was isolated using RNeasy kits and reverse transcribed into cDNA with Omniscript RT kit (both Qiagen). QPCR was performed using the Mastercycler® ep realplex2 System (Eppendorf). Platimum® Quantitative PCR SuperMix-UDG (Invitrogen) and TaqMan probes and primers were used for Actb, Il1b, Tnfa, Il6, and SsoAdvanced Universal SYBR® Green Supermix (Bio-Rad) was used for Batf3, Muc2, Irf8, 16S rRNA, A. muciniphila, M. schaedleri, Bifidobacterium spp., and Bacteroides sp. (Supplemental Table). mRNA expression of target genes was normalized to the expression of Actb. The relative gene expression was calculated by the 2-ΔΔCt method.
Hamade H., Stamps J.T., Stamps D.T., More S.K., Thomas L.S., Blackwood A.Y., Lahcene N.L., Castanon S.L., Salumbides B.C., Shimodaira Y., Goodridge H.S., Targan S.R, & Michelsen K.S. (2022). BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis. Frontiers in Immunology, 13, 841065.
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2

Quantitative PCR Expression Analysis

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Total RNA was isolated using RNeasy kits and reverse transcribed into cDNA with Omniscript RT kit (both Qiagen). QPCR was performed using the Mastercycler® ep realplex2 (link) System (Eppendorf). Platimum® Quantitative PCR SuperMix-UDG (Invitrogen) and TaqMan probes and primers were used for Actb, Il9, Il10, Il13, Batf (IDT) (Supplementary Table 7). RT2 SYBR® Green qPCR Mastermix (Qiagen) and primer sets were used for Actb, Batf3 (IDT). mRNA expression of target genes was normalized to the expression of Actb. The relative gene expression was calculated by the 2-ΔΔCt method.
Tsuda M., Hamade H., Thomas L.S., Salumbides B.C., Potdar A.A., Wong M.H., Nunnelee J.S., Stamps J.T., Neutzsky-Wulff A.V., Barrett R.J., Wang Y., Tang J., Funari V.A., Targan S.R, & Michelsen K.S. (2019). A role for BATF3 in TH9 differentiation and T cell driven mucosal pathologies. Mucosal immunology, 12(3), 644-655.
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3

Quantitative PCR Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using RNeasy kits and reverse transcribed into cDNA with Omniscript RT kit (both Qiagen). QPCR was performed using the Mastercycler® ep realplex2 (link) System (Eppendorf). Platimum® Quantitative PCR SuperMix-UDG (Invitrogen) and TaqMan probes and primers were used for Actb, Il9, Il10, Il13, Batf (IDT) (Supplementary Table 7). RT2 SYBR® Green qPCR Mastermix (Qiagen) and primer sets were used for Actb, Batf3 (IDT). mRNA expression of target genes was normalized to the expression of Actb. The relative gene expression was calculated by the 2-ΔΔCt method.
Tsuda M., Hamade H., Thomas L.S., Salumbides B.C., Potdar A.A., Wong M.H., Nunnelee J.S., Stamps J.T., Neutzsky-Wulff A.V., Barrett R.J., Wang Y., Tang J., Funari V.A., Targan S.R, & Michelsen K.S. (2019). A role for BATF3 in TH9 differentiation and T cell driven mucosal pathologies. Mucosal immunology, 12(3), 644-655.
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