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Nkg2a clone z199

Manufactured by Beckman Coulter
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NKG2A (clone Z199) is a cell surface receptor expressed on natural killer (NK) cells and a subset of T cells. It functions as an inhibitory receptor that regulates the cytotoxic activity of these cells. The NKG2A antibody (clone Z199) is a useful tool for the identification and characterization of NKG2A-expressing cells in flow cytometry and other applications.

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Lab products found in correlation

Cd3 clone sp34 2, by BD (1 mentions) Cd4 clone l200, by BD (1 mentions) Aqua blue, by Thermo Fisher Scientific (1 mentions) Cd28 clone cd28, by Beckman Coulter (1 mentions) Cd95 clone dx2, by BD (1 mentions) Hla dr, by BD (1 mentions)

Close spelling variants of this product

NKG2A (Z199, (6 citations) Anti-NKG2A-APC (clone Z199; (4 citations) NKG2A PE (clone Z199, (2 citations) Anti-NKG2A allophycocyanin (Z199 clone) (2 citations) NKG2A PE Cy7 (clone Z199; (2 citations)

2 protocols using nkg2a clone z199

1

Flow Cytometric Sorting of Lymphocyte Subsets

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The SIV infection frequency of naïve, CD28+ memory and CD28− memory CD4+ T cells and myeloid cells was determined by flow cytometrically sorting each subset. Naïve CD4+ T cells were defined as live, CD45+CD3+CD4+CD28+CD95− lymphocytes; CD28+ memory CD4+ T cells were defined as live, CD45+CD3+CD4+CD28+CD95+ lymphocytes; CD28− memory CD4+ T cells were defined as live, CD45+CD3+CD4+CD28-CD95+/- lymphocytes; myeloid cells were defined as live, CD45+CD3−CD20−NKG2AHLA-DR+. Cells were then stained with the live dead exclusion dye Aqua blue (Invitrogen) then with antibodies to CD3 (clone SP34-2, BDPharmingen), CD4 (clone L200, BDPharmingen), CD95 (clone DX2, BDPharmingen), CD28 (clone CD28.2, Beckman Coulter). CD8 (clone SK8, BDPharmingen), NKG2A (clone Z199 Beckman Coulter), CD20 (clone BDPharmingen), HLADR (clone BDPharmingen), CD14 (clone BDPharmingen), and CD45 (clone BDPharmingen).
Calantone N., Wu F., Klase Z., Deleage C., Perkins M., Matsuda K., Thompson E.A., Ortiz A.M., Vinton C.L., Ourmanov I., Loré K., Douek D.C., Estes J.D., Hirsch V.M, & Brenchley J.M. (2014). Tissue myeloid cells in SIV-infected primates acquire viral DNA through phagocytosis of infected T cells. Immunity, 41(3), 493-502.
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2

Mononuclear Cell Profiling by Flow

Cited 1 time
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Absolute numbers of mononuclear cell populations in peripheral blood were determined by flow cytometry as described (43 (link)), with the addition of anti-NK cell (CD159) antibody (NKG2A; clone Z199, Beckman Coulter).
Zhang H., Guo H., Lu L., Zahorchak A.F., Wiseman R.W., Raimondi G., Cooper D.K., Ezzelarab M.B, & Thomson A.W. (2015). Sequential monitoring and stability of ex vivo-expanded autologous and non-autologous regulatory T cells following infusion in non-human primates. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(5), 1253-1266.
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