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Synaptopodin p 19

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Synaptopodin (P-19) is a protein that is expressed in the kidneys and central nervous system. It plays a role in the regulation of the actin cytoskeleton and the structure and function of podocytes, which are specialized cells in the kidney glomerulus. This product is intended for research use only and its intended use should be evaluated by the customer.

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Lab products found in correlation

Alexa fluor 549, by Dianova (2 mentions) Cyclin d1, by Vector Laboratories (2 mentions) Lotus tetragonolobus lectin ltl fitc, by Vector Laboratories (2 mentions) Bz 9000 microscope, by Keyence (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Cyclin d1, by Abcam (2 mentions) Alexa fluor 647, by Dianova (2 mentions) Bz 2 analyzing software, by Keyence (2 mentions) Alexa fluor 488, by Dianova (2 mentions)

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Synaptopodin (28 citations)

2 protocols using synaptopodin p 19

1

Immunofluorescent Analysis of Kidney Markers

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Immunofluorescence staining was performed on 2-μm paraffin-embedded sections. CD44 (R&D Systems, Minneapolis, MN), synaptopodin (P-19; Santa Cruz Biotechnology, Dallas, TX), cyclin-D1 (ab16663; Abcam, Cambridge, UK), Ki-67 (ab16667–500; Abcam), SSeCKS (homemade by Dr. Gelman), K19 (ab15463; Abcam), K7 (ab9021; Abcam), and EGFP (ab13970; Abcam) immunostaining were performed as described previously.12 (link) The following secondary antibodies were used: donkey anti-rabbit, mouse, chicken, or rat Alexa Fluor 488, Alexa Fluor 549, or Alexa Fluor 647 (Dianova, Hamburg, Germany). The nuclei were stained using Hoechst 33342 (Sigma-Aldrich, St. Louis, MO). Sections were evaluated with a Keyence BZ-9000 Microscope using BZ-II Analyzing software (Keyence Corporation, Osaka, Japan). Analysis of distributions of EGFP, cyclin-D1, and Ki-67 on Bowman’s capsule was performed on sections counterstained with Hoechst and Fluorescein-labeled Lotus Tetragonolobus Lectin (LTL-FITC; Vector Laboratories, Burlingame, CA). At least in 25 glomeruli per kidney section, the location in relation to LTL-FITC of EGFP/cyclin-D1/Ki-67 was analyzed.
Kuppe C., Leuchtle K., Wagner A., Kabgani N., Saritas T., Puelles V.G., Smeets B., Hakroush S., van der Vlag J., Boor P., Schiffer M., Gröne H.J., Fogo A., Floege J, & Moeller M.J. (2019). Novel parietal epithelial cell subpopulations contribute to focal segmental glomerulosclerosis and glomerular tip lesions. Kidney international, 96(1), 80-93.
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2

Immunofluorescent Analysis of Kidney Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed on 2-μm paraffin-embedded sections. CD44 (R&D Systems, Minneapolis, MN), synaptopodin (P-19; Santa Cruz Biotechnology, Dallas, TX), cyclin-D1 (ab16663; Abcam, Cambridge, UK), Ki-67 (ab16667–500; Abcam), SSeCKS (homemade by Dr. Gelman), K19 (ab15463; Abcam), K7 (ab9021; Abcam), and EGFP (ab13970; Abcam) immunostaining were performed as described previously.12 (link) The following secondary antibodies were used: donkey anti-rabbit, mouse, chicken, or rat Alexa Fluor 488, Alexa Fluor 549, or Alexa Fluor 647 (Dianova, Hamburg, Germany). The nuclei were stained using Hoechst 33342 (Sigma-Aldrich, St. Louis, MO). Sections were evaluated with a Keyence BZ-9000 Microscope using BZ-II Analyzing software (Keyence Corporation, Osaka, Japan). Analysis of distributions of EGFP, cyclin-D1, and Ki-67 on Bowman’s capsule was performed on sections counterstained with Hoechst and Fluorescein-labeled Lotus Tetragonolobus Lectin (LTL-FITC; Vector Laboratories, Burlingame, CA). At least in 25 glomeruli per kidney section, the location in relation to LTL-FITC of EGFP/cyclin-D1/Ki-67 was analyzed.
Kuppe C., Leuchtle K., Wagner A., Kabgani N., Saritas T., Puelles V.G., Smeets B., Hakroush S., van der Vlag J., Boor P., Schiffer M., Gröne H.J., Fogo A., Floege J, & Moeller M.J. (2019). Novel parietal epithelial cell subpopulations contribute to focal segmental glomerulosclerosis and glomerular tip lesions. Kidney international, 96(1), 80-93.
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