1 methyl 4 phenylpyridinium iodide

Manufactured by Merck Group

Sourced in United States

1-Methyl-4-phenylpyridinium iodide is a chemical compound used as a laboratory reagent. It serves as a potent inhibitor of mitochondrial complex I, a key enzyme in the electron transport chain. The compound is commonly used in research applications involving the study of cellular energy metabolism and neurodegeneration.

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Lab products found in correlation

Echinacoside, by Merck Group (1 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide, by Merck Group (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Ex527 hy 15452, by MedChemExpress (1 mentions) 1 methyl 4 phenyl 1 2 3 6 tetrahydropyridine hydrochloride, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Chloroquine diphosphate salt, by Merck Group (1 mentions) Z vad fmk, by BD (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Uridine, by Merck Group (1 mentions) Quinidine, by Merck Group (1 mentions) Sorafenib tosylate, by MedChemExpress (1 mentions) Gemcitabine hydrochloride, by MedChemExpress (1 mentions) Cisplatin, by Merck Group (1 mentions)

4 protocols using 1 methyl 4 phenylpyridinium iodide

Echinacoside and MPTP Neuroprotection

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Echinacoside (ECH, CAS Number 82854–37-3), 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP, M0896), 1-Methyl-4-phenylpyridinium iodide (MPP⁺, D048), 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, M2128) and Chloroquine diphosphate salt (CQ, C6628) were obtained from Sigma-Aldrich. EX527 (HY-15452) was purchased from MedChemExpress. DMEM and foetal bovine serum were purchased from Gibco.

Chen C., Xia B., Tang L., Wu W., Tang J., Liang Y., Yang H., Zhang Z., Lu Y., Chen G., Yang Y, & Zhao Y. (2018). Echinacoside protects against MPTP/MPP+-induced neurotoxicity via regulating autophagy pathway mediated by Sirt1. Metabolic Brain Disease, 34(1), 203-212.

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Quantification of SH-SY5Y Cell Viability

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Viable SH-SY5Y cells were quantified by using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Images were captured using the EVOS™ FL Cell Imaging System directly from culture plates under 10× magnification. Cell death was measured by the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences) followed by LSR Fortessa II flow cytometry analysis. At least 10,000 cells were recorded per sample in each data set. In the cell death assay (Figure 3C), caspase inhibitor zVAD (Z-VAD-FMK; BD Biosciences) was added into the media upon αSyn induction (100 µM final concentration). For the cell survival assay (Figure 3E), MPP+ iodide (1-methyl-4-phenylpyridinium iodide; Sigma) was added into media of transfected cells 48 hours before processing for cell viability assay.

Chen Y.C., Farzadfard F., Gharaei N., Chen W.C., Cao J, & Lu T.K. (2017). Randomized CRISPR-Cas transcriptional perturbation screening reveals protective genes against alpha-synuclein toxicity. Molecular cell, 68(1), 247-257.e5.

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Cytotoxicity Assay Protocol

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Cisplatin, uridine, 1-methyl-4-phenylpyridinium iodide (MPP), 4-nitrobenzyl-6-thioinosine (NBTI), dipyridamole 1,1’-diethyl-2,2’-cyanine iodide (d22) and quinidine were obtained from Sigma-Aldrich (USA). Sorafenib tosylate and gemcitabine hydrochloride were purchased from MedChem express (Sweden). [5,6-³H]-uridine and [Methyl-³H]-N-methyl-4-phenylpyridinium iodide ([³H]MPP⁺) were purchased from Campro Scientific (Germany).

Urtasun N., Boces-Pascual C., Boix L., Bruix J., Pastor-Anglada M, & Pérez-Torras S. (2017). Role of drug-dependent transporter modulation on the chemosensitivity of cholangiocarcinoma. Oncotarget, 8(52), 90185-90196.

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Pd(II) meso-tetra (sulfophenyl) tetrabenzoporphyrin Preparation

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Pd(II) meso-tetra (sulfophenyl) tetrabenzoporphyrin sodium salt (C₆₀H₃₂N₄Na₄O₁₂PdS₄; catalog #T41161) was purchased from Frontier Scientific (Logan, UT, USA). Its solution (2.5 mg/mL, or 2.0 mM) was dissolved in dH₂O and stored at -20 °C. All other reagents were purchased from Sigma-Aldrich (St. Louis, MO). Glucose oxidase (10 mg/mL) was dissolved in H₂O and stored at -20 °C. Rotenone (2.0 mM) was dissolved in absolute ethanol and stored at -20 °C; its final concentration was determined using molar extinction coefficient at 294 nm of 19,200. 1-Methyl-4-phenylpyridinium iodide (MPP+, molecular weight 297.13) was purchased from Sigma Aldrich, USA. The stock solution of MPP+ (50 mM) was prepared by dissolving 29.71 mg in 2.0 mL sterile dH₂O; aliquots of the solution were stored at -80 °C.

Al Shamsi M., Haque M.E., Shahin A., Shaban S, & Souid A.K. (2021). The use of phosphorescence oxygen analyzer to measure the effects of rotenone and 1-methyl-4-phenylpyridinium on striatal cellular respiration in C57BL6 mice. Heliyon, 7(6), e07219.

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