Cytochalasin d

Manufactured by Apexbio

Sourced in United States

Cytochalasin D is a lab equipment product that functions as an actin polymerization inhibitor. It disrupts the cytoskeleton by binding to the fast-growing ends of actin filaments, preventing further polymerization.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti rabbit or anti mouse immunoglobulin g igg horseradish peroxidase hrp secondary antibodies, by Bioss Antibodies (2 mentions) β actin, by Bioss Antibodies (2 mentions) Pkh67, by Merck Group (2 mentions) Recombinant mouse il 4, by Thermo Fisher Scientific (1 mentions) Filipin 3, by Apexbio (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Rottlerin, by Apexbio (1 mentions) Fluorescein isothiocyanate (fitc), by Solarbio (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Bca protein detection kit, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Beyotime (1 mentions) Np 40 lysis buffer, by Beyotime (1 mentions) Lysophosphatidylcholine, by Merck Group (1 mentions) Antibodies against tsg101, by Abcam (1 mentions) Calnexin, by Abcam (1 mentions) Gapdh, by Abcam (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by BestBio (1 mentions)

3 protocols using cytochalasin d

Dendritic Cell Phenotype Characterization

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Ovalbumin (OVA) was purchased from Sigma-Aldrich (USA). CpG oligonucleotide 1826 (5ʹ-TCC ATG ACG TTC CTG ACG TT-3ʹ) was synthesized by Sangon (China). Fetal bovine serum (FBS), phosphate-buffered saline (PBS), RPMI-1640, DMEM were purchased from Hyclone (USA). Rottlerin, chlorpromazine, Filipin III and cytochalasin D were purchased from ApexBio Technology (USA). Anhydrous dimethyl sulfoxide (DMSO), red blood cell lysis, the carbocyanine dye Dil, 4ʹ,6-Diamidino-2-phenylindole dihydrochloride (DAPI, ≥90%), fluorescein isothiocyanate (FITC) and near-Infrared Cyanine 7 dyes (Cy7 NHS ester) were purchased from Solarbio Science & Technology Co. Ltd (China). Cell Counting Kit-8 (CCK-8), NP-40 lysis buffer, X-Gal (ST912) and BCA protein detection kit were purchased from Beyotime (China). Recombinant mouse IL-4 and GM-CSF were purchased from Peprotech (USA). Anti-mouse monoclonal antibodies (CD80 (B7-1), CD86 (B7-2), CCR7, CD40, MHC I (H-2Kb) and MHC II (I-Ab)) were purchased from Biolegend (USA). None of the chemicals were further purified.

Dong Y., Gao J., Pei M., Wang X., Zhang C., Du Y, & Jiang Y. (2020). Antigen-Conjugated Silica Solid Sphere as Nanovaccine for Cancer Immunotherapy. International Journal of Nanomedicine, 15, 2685-2697.

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Lysophosphatidylcholine-Induced Cell Signaling

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Lysophosphatidylcholine (Sigma, St. Louis, MO) was dissolved as previously described and used for cell culture studies at a concentration of 20 µM16 (link). β-actin, DR5, kidney injury molecule-1 (KIM-1) primary antibodies and goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies were purchased from Bioss (Beijing, China). Antibodies against TSG101, CD63, ARF6, calnexin, TRAIL, LRG1, and GAPDH were obtained from Abcam (Cambridge, UK). Human recombinant LRG1 and TRAIL were purchased from Abcam (Cambridge, UK). A Cr assay kit was purchased from Njjcbio (Nanjing, China). An ELISA kit for ALB was purchased from Jymbio (Wuhan, China). The Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Bestbio (Shanghai, China). Cytochalasin D was obtained from APExBIO (USA). PKH67 was obtained from Sigma (St. Louis, MO).

Jiang W.J., Xu C.T., Du C.L., Dong J.H., Xu S.B., Hu B.F., Feng R., Zang D.D., Meng X.M., Huang C., Li J, & Ma T.T. (2022). Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics, 12(1), 324-339.

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Extracellular Vesicle Characterization Protocol

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β-actin, TGFβR1 and goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies were purchased from Bioss (Beijing, China). Antibodies against Tumor susceptibility gene 101 (TSG101), CD63, Adenosine diphosphate ribosylation factor 6 (ARF6), Calnexin, and LRG1 were obtained from Abcam (Cambridge, UK). Cytochalasin D was obtained from APExBIO (USA). PKH67 was obtained from Sigma (St. Louis, MO). A83-01 and LY-364947 were purchased from MCE (USA).

Jiang W., Zhang T., Qiu Y., Liu Q., Chen X., Wang Q., Min X., Ouyang L., Jia S., Lu Q., He Y, & Zhao M. (2024). Keratinocyte-to-macrophage communication exacerbate psoriasiform dermatitis via LRG1-enriched extracellular vesicles. Theranostics, 14(3), 1049-1064.

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