Log In Sign up
The largest database of trusted experimental protocols

β actin a2066

Manufactured by Merck Group
Visit Supplier
Sourced in United States

β-actin (A2066) is a cytoskeletal protein that is highly conserved across various species. It is commonly used as a reference gene or loading control in molecular biology experiments, such as Western blotting, qRT-PCR, and immunocytochemistry, to normalize the expression of target genes or proteins.

Automatically generated - may contain errors

Lab products found in correlation

Tissuelyser 2, by Qiagen (1 mentions) Flag m2, by Merck Group (1 mentions) Phospho s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Nupage novex 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Iblot dry blotting system, by Thermo Fisher Scientific (1 mentions) C myc y69, by Abcam (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) N myc ncm 2 100, by Abcam (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204 d13.14.4e, by Cell Signaling Technology (1 mentions) Gapdh, by BioLegend (1 mentions) Stat3 9139, by Cell Signaling Technology (1 mentions) Caco 2 atcc htb 37, by American Type Culture Collection (1 mentions) Carboxy h2dcfda c400, by Thermo Fisher Scientific (1 mentions) Chk2 pthr 68, by Cell Signaling Technology (1 mentions) Atm pser 1981, by Cell Signaling Technology (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Iose80, by American Type Culture Collection (1 mentions) Ki67 antibody ab1667, by Abcam (1 mentions) Pelp1 antibody a300 180a, by Fortis Life Sciences (1 mentions)

Close spelling variants of this product

β-actin (6886 citations) A2066 (195 citations) Actin (A2066) (18 citations) Anti-β-actin (A2066) (9 citations) Rabbit anti-β-actin (A2066) (2 citations)

5 protocols using β actin a2066

1

Western Blot Analysis of Protein Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells and xenograft tumor samples were resuspended in high SDS-RIPA Buffer (50 mM Tris-HCl, pH 7.5, 150 mM Sodium Chloride, 1 % Triton X-100, 1 % sodium deoxycholate, 1 % SDS, 2 mM EDTA; Sigma Aldrich). Tissues were disrupted and homogenized with a TissueLyser II (Qiagen) for 2 × 2 min intervals at 30Hz. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit (Pierce). A total of 15–50 μg of protein extracts were loaded onto NuPAGE® Novex® 4–12 % Bis-Tris Protein Gels (Life Technologies) and subsequently transferred onto nitrocellulose membranes using the iBlot® Dry Blotting System (Life Technologies). The blots were developed using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Scientific). Antibodies: S6-Ribosomal protein (5G10), Phospho-S6 Ribosomal protein (Ser240/244) (D68F8), Phospho-4E-BP1 (Thr37/46) (236B4), p44/42 MAPK (Erk1/2) (137 F5), and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) were purchased from Cell Signaling Technology. C-MYC (Y69) and N-MYC (NCM II 100) were purchased from Abcam. FLAG (M2) and β-actin (A2066) antibodies were purchased from Sigma Aldrich.
Dela Cruz F.S., Diolaiti D., Turk A.T., Rainey A.R., Ambesi-Impiombato A., Andrews S.J., Mansukhani M.M., Nagy P.L., Alvarez M.J., Califano A., Forouhar F., Modzelewski B., Mitchell C.M., Yamashiro D.J., Marks L.J., Bender J.L, & Kung A.L. (2016). A case study of an integrative genomic and experimental therapeutic approach for rare tumors: identification of vulnerabilities in a pediatric poorly differentiated carcinoma. Genome Medicine, 8, 116.
+ Open protocol
+ Expand
2

Mammary Gland Protein Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total mammary gland and tumor protein extracts were obtained from snap frozen tissue that was pulverized under liquid N2 as described [35 (link)]. After the addition of RIPA buffer (50 mM Tris–HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, pH 7.5, 10 μg/ml aprotinin, 1 μM pepstatin, 1 μM leupeptin, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM Na2VO4), the samples were sonicated then incubated on ice for 30 min. Insoluble material was removed by centrifugation at 9000 g for 20 min at 4 °C. Protein samples were aliquoted and frozen at − 80C. Densitometry was performed using ImageJ.
Antibodies: β-actin #A-2066; SMA #ABT1487 (both Sigma), P-Stat3 #9134, Stat3 #9139, p53(D2H90) #32532S, Bim #2433S (all from Cell Signaling), Puma #ab54288, vinculin #ab129003, Smad3 #ab84177, (all Abcam); gapdh (Biolegend, #MMS-5805); NF-κB2(p52) (#06–413), Bcl-2 (#5826) (both Upstate); RelB (C-19) #sc-226, p65/RelA #sc-109, p50(H-119) #sc7178, Id3(2B11) #sc-56712, (all Santa Cruz).
Carr D., Zein A., Coulombe J., Jiang T., Cabrita M.A., Ward G., Daneshmand M., Sau A, & Pratt M.A. (2022). Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology. Breast Cancer Research : BCR, 24, 40.
+ Open protocol
+ Expand
3

Oxidative Stress in CaCo-2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human colon epithelial cells CaCo-2 (ATCC®HTB-37™) were obtained from the American Type Culture Collection (Rockville, MD, USA). The compounds for cell cultures were purchased by Euroclone (Euroclone, Italy). Carboxy-H2DCFDA (C400) was supplied by Invitrogen (Invitrogen, Carlsbad, CA, USA). Goat polyclonal anti-AGE (AB9890) antibodies and rabbit polyclonal β-actin (A2066) were purchased by Sigma Aldrich (Sigma, St Louis, MO, USA). All other reagents have also been purchased from Sigma Aldrich (Sigma, St Louis, MO, USA).
Cianfruglia L., Morresi C., Bacchetti T., Armeni T, & Ferretti G. (2020). Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway. Antioxidants, 9(10), 1006.
+ Open protocol
+ Expand
4

Protein Profiling by Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates, SDS-PAGE and Western blot were performed as described in [5 (link)]. Antibodies used were: ATM p-Ser1981 #13050, Chk2 p-Thr68 #2661, Chk2 #2662, Chk1 p-Ser345 #2341, Chk1 #2360, KAP1 #5868, cleaved caspase 3 (Asp175) #9661 (Cell Signaling Technology, Danvers, MA, USA), KAP1 p-Ser473 #644602 (Biolegend, San Diego, CA, USA), ATM #AF1655 (R&D Systems Bio-Techne, Minneapolis, MN, USA), vinculin #V9131, β-actin #A2066 (Millipore Sigma, Burlington, MA, USA). Detection was performed by exposure to standard X-ray films or by a CCD camera system (UVITEC, Cambridge, UK).
Magni M., Paolizzi C., Monfrini C., Vella C., Corradini P, & Carniti C. (2022). Targeting the DNA Damage Response to Increase Anthracycline-Based Chemotherapy Cytotoxicity in T-Cell Lymphoma. International Journal of Molecular Sciences, 23(7), 3834.
+ Open protocol
+ Expand
5

Characterization of Breast Cancer Cell Lines

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human WT- ER+ BC cells MCF7, ZR75, and T47D, human mammary epithelial cells (HMEC), murine mammary epithelial cells (HC11), primary human endometrial epithelial cells (HEEC), primary human endometrial stromal cells (HESC), and primary human nontumorigenic immortalized ovarian surface epithelial cells (IOSE-80) were obtained from the American Type Culture Collection (ATCC) and were maintained using ATCC recommended media. MCF7 and ZR75 model cells stably overexpressing PELP1 cDNA or PELP1-shRNA have been described previously (18 (link)). MCF7-TamR (tamoxifen resistant) and MCF7-LTLT (letrozole resistant) cells were cultured in tamoxifen or letrozole (1 μM) containing media (19 ). MCF7(Y537S) and MCF7(D538G) MT- ER+ BC cell lines were described previously (20 (link)). ZR75(Y537S) and ZR75(D538G) MT- ER+ BC cell lines were generated in our lab (19 ). All model cells utilized were free of mycoplasma contamination. Additionally, STR DNA profiling was used to confirm the identity of cells. The GAPDH (8884) antibody was obtained from Cell Signaling Technology (Beverly, MA). The β-Actin (A-2066) and Vinculin antibodies (V9264) were purchased from Millipore Sigma (Burlington, MA). The Ki67 antibody (ab1667) was purchased from Abcam (Cambridge, MA). The PELP1 antibody (A300-180A) was purchased form Bethyl Laboratories Inc. (Montgomery, TX).
Altwegg K.A., Viswanadhapalli S., Mann M., Chakravarty D., Krishnan S., Liu Z., Liu J., Pratap U.P., Ebrahimi B., Sanchez J.R., Li X., Ma S., Park B.H., Santhamma B., Chen Y., Lai Z., Raj G.V., Yuan Y., Zhou D., Sareddy G.R., Tekmal R.R., McHardy S., Huang T.H., Rao M.K., Vankayalapati H, & Vadlamudi R.K. (2022). A first-in-class inhibitor of ER coregulator PELP1 targets ER+ breast cancer. Cancer research, 82(20), 3830-3844.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!