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Sars cov 2 igg elisa kit

Manufactured by EUROIMMUN
Sourced in United States, Germany

The SARS-CoV-2 IgG ELISA kit is a laboratory assay used for the detection of IgG antibodies against the SARS-CoV-2 virus. It is designed to assist in the serological detection of previous exposure to the SARS-CoV-2 virus.

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2 protocols using sars cov 2 igg elisa kit

1

Comparative Evaluation of SARS-CoV-2 Antibody Assays

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Five serological tests were performed in all three rounds. Characteristics of the tests are provided in Supplementary Table S1. Two of the tests detected antibodies recognizing the S-antigen, one recognized the 2019-nCoV recombinant antigen and two tests recognized the N-antigen of SARS-CoV-2. Detection of SARS-CoV-2 IgG antibodies was performed with five different quantification methods, of which two were enzyme-linked immunosorbent assays (ELISA) and three were chemiluminescence-based immunoassays (CLIA/CMIA). All tests were carried out according to manufacturers’ instructions. For detailed information on assay characteristics and instruments used, see Supplementary Table S1. Sensitivities and specificities are shown as provided by the manufacturer. The following assays were used; EDI Novel Coronavirus SARS-CoV-2 IgG ELISA kit (Epitope Diagnostics Inc., San Diego, CA, USA), SARS-CoV-2 IgG ELISA kit (Euroimmun, Lübeck, Germany), SARS-CoV-2 S1/S2 IgG CLIA kit (DiaSorin, Saluggia, Italy), 2019-nCoV IgG kit (Snibe Co., Ltd., Shenzhen, China) and Elecsys Anti–SARS-CoV-2 kit (Roche, Basel, Switzerland).
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2

SARS-CoV-2 IgG ELISA Assay on Inactivated Plasma

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Frozen plasma was thawed, heat inactivated for 30 min at 56°C, centrifuged for 5 min at 5,000 × g, and the supernatant transferred to a fresh tube. Each sample was tested with the Euroimmun SARS-CoV-2 IgG ELISA kit (EI 2606-9620 G, Euroimmun, Germany) and sample dilution was performed according to the manufacturer's instructions. ELISA of the plasma samples from ICU patients was only possible for 32 individuals due to the lack of enough sample volume.
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