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Analytical reagent sucrose

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Analytical Reagent Sucrose is a high-purity sucrose product intended for use in laboratory analysis and testing applications. It provides a consistent and reliable source of sucrose for accurate and reproducible results.

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Lab products found in correlation

Ivermectin, by Merck Group (1 mentions) Imidacloprid, by Bayer (1 mentions) Chlorantraniliprole, by DuPont (1 mentions) Confidor, by Bayer (1 mentions) Nunc cell plates, by Thermo Fisher Scientific (1 mentions) Spinosad, by Merck Group (1 mentions)

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Sucrose (13 citations)

6 protocols using analytical reagent sucrose

1

Antioxidant NACA Pretreatment for Spinosad Exposure

Cited 1 time
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The antioxidant, NACA, was used as previously described (Martelli et al., 2020 (link)). Briefly, larvae were treated with 300 µg/mL of NACA in 5% Analytical Reagent Sucrose (Chem Supply) solution for 5 hr prior to exposure to spinosad exposures.
Martelli F., Hernandes N.H., Zuo Z., Wang J., Wong C.O., Karagas N.E., Roessner U., Rupasinghe T., Robin C., Venkatachalam K., Perry T., Batterham P, & Bellen H.J. (2022). Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in flies. eLife, 11, e73812.
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2

Pesticide Dilution Protocol for Toxicity Assays

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Chlorantraniliprole (10 gL-1 Coragen®; Du Pont), Imidacloprid (200 gL-1 Confidor®; Bayer Crop Science), and Spinosad (10 gL-1 Success®; Yates) were all purchased commercially and diluted to 5,000ppm stocks using distilled water. On the day of exposure, 5x stocks were generated for each dose being used (Chlorantraniliprole: 60, 30, 15, 7.5, 3.75, 0ppm; Imidacloprid: 240, 120, 60, 30, 15, 0ppm; Spinosad: 240, 60, 30, 15, 7.5, 3.75, 0 ppm) by diluting the 5,000ppm stock in 5% Analytical Reagent sucrose (Chem Supply). A similar procedure was followed for Ivermectin (Sigma), and a 10,000ppm stock was generated using DMSO as a solvent. 5x stocks were generated (120, 60, 30, 15, 7.5, 0 ppm) by dissolving the original stock in 5% sucrose, and the highest concentration of DMSO used in dosing was added to the 0ppm solution in order to control for solvent effects, none of which were observed.
Denecke S., Nowell C.J., Fournier-Level A., Perry T, & Batterham P. (2015). The Wiggle Index: An Open Source Bioassay to Assess Sub-Lethal Insecticide Response in Drosophila melanogaster. PLoS ONE, 10(12), e0145051.
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3

Spinosad Dilution and Preparation

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Pure spinosad (Sigma-Aldrich) was used in all assays. The chemical was diluted to create 1000 ppm stocks solution, using dimethyl sulfoxide (DMSO), and was kept on freezer (–20°C). Before exposures, 5× stocks were generated for the dose being used by diluting the 1000 ppm stock in 5% Analytical Reagent Sucrose (Chem Supply) solution (or equivalent dose of DMSO for controls).
Martelli F., Hernandes N.H., Zuo Z., Wang J., Wong C.O., Karagas N.E., Roessner U., Rupasinghe T., Robin C., Venkatachalam K., Perry T., Batterham P, & Bellen H.J. (2022). Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in flies. eLife, 11, e73812.
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4

Larval Motility Response to Insecticide

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Larval movement in response to insecticide exposure was quantified by Wiggle Index Assay, as described by Denecke et al., 2015 (link). 25 third-instar larvae were used for a single biological replicate and 4 replicates were tested for each exposure condition. Undosed larvae in NUNC cell plates (Thermo Scientific) in 5% Analytical Reagent Sucrose (Chem Supply) solution were filmed for 30 s, and then 30 min, 1 hr, 1 hr, and 30 min and 2 hr after spinosad exposure. The motility at each time point is expressed in terms of relative movement ratio (RMR), normalized to motility prior to spinosad addition.
Martelli F., Hernandes N.H., Zuo Z., Wang J., Wong C.O., Karagas N.E., Roessner U., Rupasinghe T., Robin C., Venkatachalam K., Perry T., Batterham P, & Bellen H.J. (2022). Low doses of the organic insecticide spinosad trigger lysosomal defects, elevated ROS, lipid dysregulation, and neurodegeneration in flies. eLife, 11, e73812.
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5

Spinosad Bioassay Protocol

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The pure version of spinosad (Sigma Aldrich®) was used in all assays. The chemical was diluted to create 1000 ppm stocks solution, using dimethyl sulfoxide (DMSO), and was kept on freezer (-20°C).
Before exposures, 5x stocks were generated for the dose being used by diluting the 1000 ppm stock in 5% Analytical Reagent Sucrose (Chem Supply) solution (or equivalent dose of DMSO for controls).
Martelli F., Zuo Z., Wang J., Wong C., Karagas N.E., Roessner U., Rupasinghe T., Venkatachalam K., Perry T., Batzoglou S., & Bellen H.J. (2021). Low doses of the organic insecticide spinosad trigger lysosomal defects, ROS driven lipid dysregulation and neurodegeneration in flies.
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6

Antioxidant NACA treatment in larvae

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The antioxidant, N-acetylcysteine amide (NACA) was used as previously described (Martelli et al., 2020) (link). Briefly, larvae were treated with 300 µg/mL of NACA in 5% Analytical Reagent Sucrose (Chem Supply) solution for 5 hr prior to exposure to spinosad exposures.
Martelli F., Zuo Z., Wang J., Wong C., Karagas N.E., Roessner U., Rupasinghe T., Venkatachalam K., Perry T., Batzoglou S., & Bellen H.J. (2021). Low doses of the organic insecticide spinosad trigger lysosomal defects, ROS driven lipid dysregulation and neurodegeneration in flies.
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