Isoeugenol and ferulic acid from Sinopharm Group (Beijing, China), 4-vinylguaiacol and vanillin from Sigma-Aldrich (St. Louis, MO, USA) were all dissolved in dimethyl sulfoxide as stock solutions of 500 mM. The dimethyl sulfoxide, trichloromethane and other organic compounds were obtained from Sinopharm Group (Beijing, China). Standard protein marker for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), polymerase chain reaction (PCR) buffers, FastPfu DNA polymerase, and restriction enzymes were all obtained from Takara (Japan). The kits used for bacterial plasmid extraction, gel extraction and PCR purification were all purchased from Transgene (Beijing, China). All other chemicals were analytical grade.
Dimethyl sulfoxide (dmso)
Manufactured by Sinopharm
Sourced in China, United States
Dimethyl sulfoxide (DMSO) is a colorless, odorless, and highly polar organic solvent. Its primary function is to serve as a versatile solvent for a wide range of organic and inorganic compounds, facilitating various chemical reactions and extraction processes in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Sinopharm (29 mentions)
Ethanol, by Sinopharm (26 mentions)
Acetone, by Sinopharm (19 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (18 mentions)
Sodium hydroxide (naoh), by Sinopharm (17 mentions)
Chloroform, by Sinopharm (15 mentions)
Sodium chloride (nacl), by Sinopharm (15 mentions)
Hydrochloric acid (hcl), by Sinopharm (14 mentions)
N n dimethylformamide (dmf), by Sinopharm (12 mentions)
Acetonitrile, by Sinopharm (10 mentions)
Dichloromethane, by Sinopharm (9 mentions)
Toluene, by Sinopharm (9 mentions)
Acetic acid, by Sinopharm (7 mentions)
N hexane, by Sinopharm (7 mentions)
Ethyl acetate, by Sinopharm (7 mentions)
Sulfuric acid, by Sinopharm (6 mentions)
Diethyl ether, by Sinopharm (6 mentions)
Anhydrous ethanol, by Sinopharm (6 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
Absolute ethanol, by Sinopharm (6 mentions)
225 protocols using dimethyl sulfoxide (dmso)
1
Extraction and Characterization of Bioactive Compounds
2
Isoeugenol and Guaiacol Compounds Purification
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Isoeugenol was purchased from the Macklin Group (Shanghai, China); 4-vinylguaiacol was obtained from the LID Group (Shijiazhuang, China); vanillin was provided from Sigma-Aldrich (St. Louis, MO, United States). Isoeugenol and 4-vinylguaiacol were dissolved in dimethyl sulfoxide as stock solutions of 500 mM. The dimethyl sulfoxide and other organic compounds were obtained from Sinopharm Group (Beijing, China). The standard protein marker for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was purchased from Takara (Dalian, China). The kits used for bacterial plasmid extraction was purchased from Transgene (Beijing, China). All other chemicals were of analytical grade.
3
Synthesis of PVDF-Nanosilica Composites
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The poly(vinylidene fluoride) (PVDF, KF850) was purchased from Kureha Chemicals Japan (Ibaraki, Japan) and dried in vacuum at 80 °C for 24 h before use. Nanosilica with a mean size of 10 nm was purchased from Carbot bluestar Co. China (Jiujiang, China) and dried in vacuum at 200 °C for 12 h before use. Acetone (≥98.0%), Dimethyl sulfoxide (DMSO, ≥98.0%), N, N-Dimethylformamide (DMF, ≥99.5), Dimethylsulfoxide (DMSO, ≥99.5), and Ethanol absolute (≥99.8%) andγ-methacryloxy propyl-trimethoxyl silane (γ-MPS, ≥98.0%) were all purchased from a Sinopharm Chemical Reagent (Shanghai, China). All reactions were carried out under a nitrogen condition unless otherwise stated.
4
Bismuth-Copper Nanoparticle Synthesis Protocol
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Materials. Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, 99%), copper nitrate trihydrate (Cu(NO3)2·3H2O, 99%), TAA, dimethyl sulfoxide (DMSO), nitric acid (HNO3), methylene blue (MB, ≥82%) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). DATS (≥98%) was bought from Beijing Jin Ming Biotechnology Co., Ltd. BSA, GSH, 3,3′,5,5′-tetramethylbenzidine (TMB), terephthalic acid (TPA) and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich. No further purification for all used reagents were operated. The catalog numbers of reagents: Bi(NO3)3·5H2O (Sinopharm Chemical Reagent Co., Ltd, catalog number: 80018318), Cu(NO3)2·3H2O (Sinopharm Chemical Reagent Co., Ltd, catalog number: 10007916), TAA (Sinopharm Chemical Reagent Co., Ltd, catalog number: 30177714), DMSO (Sinopharm Chemical Reagent Co., Ltd, catalog number: 30072428), DATS (Beijing Jin Ming Biotechnology Co., Ltd, catalog number: D60006), nitric acid (Sinopharm Chemical Reagent Co., Ltd, catalog number: 10014528), BSA (Sigma-Aldrich, B265993-25g), MB (Sinopharm Chemical Reagent Co., Ltd, catalog numbers: 71024544), GSH (Sigma-Aldrich, G105427-5g), 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich, T100417), TPA (Sigma-Aldrich, P108506-100g), 5,5′-dithiobis-(2-nitrobenzoic acid) (Sigma-Aldrich, D105559).
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Materials. Bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, 99%), copper nitrate trihydrate (Cu(NO3)2·3H2O, 99%), TAA, dimethyl sulfoxide (DMSO), nitric acid (HNO3), methylene blue (MB, ≥82%) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). DATS (≥98%) was bought from Beijing Jin Ming Biotechnology Co., Ltd. BSA, GSH, 3,3′,5,5′-tetramethylbenzidine (TMB), terephthalic acid (TPA) and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Sigma-Aldrich. No further purification for all used reagents were operated. The catalog numbers of reagents: Bi(NO3)3·5H2O (Sinopharm Chemical Reagent Co., Ltd, catalog number: 80018318), Cu(NO3)2·3H2O (Sinopharm Chemical Reagent Co., Ltd, catalog number: 10007916), TAA (Sinopharm Chemical Reagent Co., Ltd, catalog number: 30177714), DMSO (Sinopharm Chemical Reagent Co., Ltd, catalog number: 30072428), DATS (Beijing Jin Ming Biotechnology Co., Ltd, catalog number: D60006), nitric acid (Sinopharm Chemical Reagent Co., Ltd, catalog number: 10014528), BSA (Sigma-Aldrich, B265993-25g), MB (Sinopharm Chemical Reagent Co., Ltd, catalog numbers: 71024544), GSH (Sigma-Aldrich, G105427-5g), 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich, T100417), TPA (Sigma-Aldrich, P108506-100g), 5,5′-dithiobis-(2-nitrobenzoic acid) (Sigma-Aldrich, D105559).
5
Synthesis and Characterization of Nanomaterials
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Chloroauric acid tetrahydrate (HAuCl4·4H2O, AR), ascorbic acid (AA, AR), dimethyl sulfoxide (DMSO, AR), and ammonium molybdate tetrahydrate (AMTH, AR) were purchased from Sinopharm Chemical Co., Ltd. (Shanghai, China). 5,5-dimethyl-1-pyrroline n-oxide (DMPO, 97%) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Methylene blue (MB, BS) was derived from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Thiourea (99%) was obtained from Alfa Aesar (Haverhill, MA, USA). Hydrogen peroxide (H2O2) (30%, aqueous solution, AR) was obtained from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). All the water used was Milli-Q water (≥18.2 MΩ·cm).
6
Biocatalytic Synthesis Using Renewable Feedstocks
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Bovine serum albumin (BSA), NADH, and NAD + were purchased from Roche (China). Glucose, ammonium sulfate, lactic acid, formic acid, acetic acid, choline chloride, sodium hydroxide, dimethyl sulfoxide, epichlorohydrin, sodium thiosulfate, ethanediamine, glutaraldehyde, and sodium metaperiodate were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Dextran (MW 70,000) was purchased from Energy Chemical (Shanghai, China). Polyethyleneimine solution (PEI, MW~1,300) was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd (China). One hundred meshes of cornstalk, pasture, wheat straw, rice husk, and poplar powder were purchased from XingYi Deep Processing Factory (China). 2-Cyclohexen-1-one was purchased from Chengdu Best Chemical Reagent Co., Ltd (China). The substrate 4-(4-Methoxyphenyl)-3-buten-2-one and the product 4-(4-Methoxyphenyl)butan-2-one were synthesized and purified in our laboratory [26] . ER and GDH were constructed and expressed in our laboratory [26] . The deep eutectic solvents (DESs) were prepared in our laboratory [27] . The other chemicals used in this study were of analytical grade.
7
Synthesis and Characterization of Polymeric Sorbents
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Reagents: Auramine O hydrochloride (AOHCl, 90%), 2,2-Azobisisobutyronitrile (AIBN), divinylbenzene (DVB), ethylene glycol dimethacrylate (EDMA) and methacrylic acid (MAA) were purchased from J&K Chemicals (Beijing, China). Itaconic acid (IA) was supplied by Sigma-Aldrich (Shanghai, China). Trimethylolpropane trimethacrylate (TRIM) was purchased from Acros Organics, USA. Ethanol, acetonitrile, glacial acetic acid, mEthanol, dimethylsulfoxide (DMSO) and N,N-dimethylformamide (DMF), 2,2-Azobisisobutyronitrile were obtained from Sinopharm Chemical Reagent. All the chemicals are analytical reagent except for mentioned.
Instrumentation: All reagents were weighed using an electronic analytical balance (BS210S, Beijing Sartorius Balance). Evaporation was achieved by rotary evaporator (RE-2000B, Shanghai Yarong Biochemistry Instrument Factory). The vacuum conditions were achieved by a vacuum oven (DZF-6020, Shanghai Jinghong Laboratory Instrument). Centrifugation was achieved with a high-speed centrifuge (HC-2062, Anhui USTC Zonkia Scientific Instruments). Constant temperature was achieved with electric thermostatic water bath (8002, Beijing Titan Instrument). UV-Vis spectroscopy was performed with the aid of UV-1800 spectrophotometer (Shimadzu, Japan). FTIR spectroscopy was performed using KBr pellets on a EQUINOX 55 (Bruker Optics, Germany) in the spectra range of 400-4000 cm -1 .
Instrumentation: All reagents were weighed using an electronic analytical balance (BS210S, Beijing Sartorius Balance). Evaporation was achieved by rotary evaporator (RE-2000B, Shanghai Yarong Biochemistry Instrument Factory). The vacuum conditions were achieved by a vacuum oven (DZF-6020, Shanghai Jinghong Laboratory Instrument). Centrifugation was achieved with a high-speed centrifuge (HC-2062, Anhui USTC Zonkia Scientific Instruments). Constant temperature was achieved with electric thermostatic water bath (8002, Beijing Titan Instrument). UV-Vis spectroscopy was performed with the aid of UV-1800 spectrophotometer (Shimadzu, Japan). FTIR spectroscopy was performed using KBr pellets on a EQUINOX 55 (Bruker Optics, Germany) in the spectra range of 400-4000 cm -1 .
8
Engineered Porous Scaffolds for Cartilage Regeneration
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Tetraethyl orthosilicate (TEOS), branched polyethyleneimine (PEI, 25 kDa), N-hydroxysuccinimide (NHS), cetyltrimethylammonium chloride solution (CTAC), triethylamine (TEA), and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent (Shanghai, China). VEGF was obtained from PeproTech (Cranbury, NJ, USA). DMEM, trypsin, type Ⅱ collagenase, penicillin/streptomycin, fetal bovine serum, and Alizarin red were purchased from Sangon Biotech (Shanghai, China). Porcine aortic valves were purchased from Songlin Meat Food Co., Ltd. A Cell Counting Kit-8 (CCK-8) and a BCIP/NBT ALP staining kit (Beyotime, Shanghai, China). An alkaline phosphatase assay kit (Jiancheng Bioengineering Institute, Nanjing, China), a calcium content detection kit (MingDian, Shanghai, China), and an RNeasy mini kit were purchased from Qiagen (Duesseldorf, Germany), IL-1, IL-6, IL-10, and TNF-α kit (R&D, Santa Clara, CA, USA). Primary antibodies (GAPDH, OPN, OSX, RunX2, α-SAM, Vimentin, and CD31) and donkey anti-rabbit IgG were procured from Abcam (Cambridge, UK). LipofectamineTM 3000 Transfection Reagent was purchased from Invitrogen (Carlsbad, CA, USA).
9
Formulation and Characterization of AgSD-Loaded Pluronic Hydrogel
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AgSD was purchased from Jinyu Pharmacy Company (Nanyang, People’s Republic of China). P407 and poloxamer 188 (P188) were purchased from BASF, Ludwigshafen, Germany. Glycerol (Gly), ethanol, ammonium solution, dimethyl sulfoxide (DMSO), phenol, and phosphoric acid were ordered from Sinopharm Chemical Reagent Co (Beijing, People's Republic of China). Lauryl sodium sulfate was procured from VWR International, Radnor, PA, USA. Methyl thiazolyltetrazolium (MTT) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Acetonitrile and methanol were obtained from Thermo Fisher Scientific (Waltham, MA, USA) with an HPLC grade. All other reagents were of analytical grade. Purified water used in this study was prepared using a Mille-Q (EMD Millipore, Billerica, MA, USA).
10
Rutin and MPBA-Mediated Silver Nanoparticle Synthesis
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Rutin and 4-mercaptophenylboronic acid (MPBA) were obtained from Aladdin Reagent Co. (China). Silver nitrate, sodium borohydride (NaBH4), NH4HCO3, and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co., Ltd. (China). Heparin sodium was provided by Sangon Biotech (Shanghai) Co., Ltd. Polyvinylpyrrolidone (PVP, Mw: 10 kDa), carrageenan, and urethane were obtained from Sigma-Aldrich Co. (United States). Endothelial cell basal medium (ECM), fetal bovine serum (FBS), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) were purchased from Thermo Fisher Scientific Co. (United States).
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