Lc msd trap vl ion trap mass spectrometer

LC–MS analysis was performed on same HPLC system after coupling to LC-MSD trap VL ion-trap mass spectrometer (Agilent, United States) equipped with an ESI. The mass signal dimension was 50–1100 m/z. Anthocyanins were ionized in positive mode. LC–MS analytical conditions were set as follows: scan range: 50–1100 m/z; capillary voltage: 4000 V; nebulization pressure: 241.3 kPa; drying gas (nitrogen); temperature: 350°C; flow rate: 8.0 L/min; capillary exit voltage: 116.7 V; capillary offset voltage: 75.9 V.

HPLC‐ESI‐MS analysis was carried out on an Agilent 1100 HPLC/MS(SL) system equipped with a UV detector and a LC‐MSD Trap VL ion‐trap mass spectrometer (MS) via an electrospray ionization (ESI) interface (Agilent Technologies). HPLC separation conditions were the same as described above. For MS conditions, the ESI capillary voltage was 3.0 kV in negative ion (NI) mode with the capillary temperature at 350°C. A nebulizing gas of 1.5 L/min and a drying gas of 10 L/min were applied for ionization using nitrogen (N₂). ESI was performed with the scan range at m/z 150–1000.

High-Performance Liquid Chromatography-ElectroSpray Ionization-Mass Spectrometry (HPLC-ESI-MS) analyses of the lignans were performed using an Agilent-1100 HPLC system equipped with a UV detector coupled to a LC-MSD Trap VL ion-trap mass spectrometer with an ESI source (Agilent Technologies, Palo Alto, CA, USA). The HPLC separation conditions were identical to those used for HPLC-DAD analysis. Negative-ion (PI) mode was used for the MS analysis of lignans.
ESI was performed using the following conditions: capillary voltage, 5.0 kV; nebulization pressure, 241.3 kPa; gas (N²) temperature, 350°C; and flow rate, 8.0 L min^-1. The capillary offset and exit voltage were 68.4 V and 100.6 V, respectively. The skim 1 voltage was 29.5 V, the skim 2 voltage was 6.0 V, and the MS and MS² spectra were recorded over an m/z range of 150 to 2000 NI. The MS² scan of the target ions used normalized collision energy of 20%–45%. The experimental standards of Schizandrol A, Schizandrol B, Schizandrin B, Schizandrin A, and isoschizandrin were purchased from Sigma-Aldrich (Steinheim, Germany); data obtained for experimental standards and information regarding the identified compounds were combined to identify the lignans.