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G 6983

Manufactured by Cayman Chemical
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Gö6983 is a protein kinase C (PKC) inhibitor. It is a chemical compound used in laboratory research settings to study the role of PKC in various cellular processes.

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Lab products found in correlation

Incb3284, by Cayman Chemical (2 mentions) Phospho ikba ser32, by Cell Signaling Technology (2 mentions) Cd154, by Santa Cruz Biotechnology (2 mentions) Nfatc1, by BioLegend (2 mentions) Bay11 7082, by Enzo Life Sciences (2 mentions) Pi3k p85, by Cell Signaling Technology (1 mentions) Ldh assay, by Promega (1 mentions) Rottlerin, by Cayman Chemical (1 mentions) Phospho pi3k, by Cell Signaling Technology (1 mentions) Phospho pkcδ, by Cell Signaling Technology (1 mentions) Bisindolylmaleimide 1, by Cayman Chemical (1 mentions) Wortmannin, by Cayman Chemical (1 mentions) Ly294002, by Cayman Chemical (1 mentions) Recombinant human ccl2, by Thermo Fisher Scientific (1 mentions) Mg132, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Thermo Fisher Scientific (1 mentions) Aeb071, by MedChemExpress (1 mentions) G 6976, by Enzo Life Sciences (1 mentions) Tpca 1, by Cayman Chemical (1 mentions) Bay11 7082, by R&D Systems (1 mentions)

8 protocols using g 6983

1

Signaling Pathways in Cell Activation

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The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
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2

Integrin-mediated Signaling Pathway Analysis

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All reagents unless otherwise specified were from Sigma-Aldrich. Wortmannin, Ly294002, rottlerin, bisindolylmaleimide i, bisindolylmaleimide ix, Gö6983 were from Cayman Chemical. P11, GRGDSP, GRADSP, αvβ3 function blocking antibody (LM609) were from EMD Millipore. PP1, PP2, PP3 were from Calbiochem. LDH assay was from Promega. Phospho-MARCKS Ser152/156 (Cat#2741), MARCKS (Cat#5607), Phospho-PI3K (Cat#4228), p85 PI3K (Cat#4257), Phospho-PKCδ (Cat#2055), PKCd (Cat#9616) antibodies were from Cell Signaling Technology. FITC-PIP3 antibody (Cat#G345) was from Echelon. All secondary antibodies and isotype controls were from Life Technologies.
Cornick S., Moreau F, & Chadee K. (2016). Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin. PLoS Pathogens, 12(4), e1005579.
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3

PMA-Mediated Osteogenesis Regulation

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PMA (phorbol 12-myristate 13-acetate) was purchased from Adipo Gen Life Sciences, Inc. (Cat. No. AG-CN2-0010-M001). BMP-2 human recombinant protein was purchased from R&D Systems, Inc. (Cat. No.355-BEC-010) and Gö6983 from Cayman Chemical, Inc. (Cat. No.13311). pCMV6-Entry (Cat. No. PS100001) and Npnt-pCMV6-Entry (Cat. No. MR208888) vectors were purchased from ORIGENE.
Kinoshita M., Yamada A., Sasa K., Ikezaki K., Shirota T, & Kamijo R. (2021). Phorbol-12-myristate 13-acetate inhibits Nephronectin gene expression via Protein kinase C alpha and c-Jun/c-Fos transcription factors. Scientific Reports, 11, 20360.
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4

Investigating CCL2 Signaling Pathway

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PP2 was obtained from Tocris (cat# 1407). Gö 6983 was purchased from Cayman Chemical (cat# 13311). Recombinant human CCL2 was purchased from Peprotech (cat# 300–04).
Yao M., Fang W., Smart C., Hu Q., Huang S., Alvarez N., Fields P, & Cheng N. (2018). CCR2 chemokine receptors enhance growth and cell cycle progression of breast cancer cells through SRC and PKC activation. Molecular cancer research : MCR, 17(2), 604-617.
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5

Investigating PMA-Induced Signaling Pathways

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2.5 × 105 cells were plated in a 6-well plate 1 day prior to drug treatment. PMA was then added to the media and incubated at 37°C with 5% CO2 for 6 hours unless otherwise indicated. For experiments utilizing inhibitors, cells were pretreated with inhibitors 30 minutes prior to PMA induction (25ng/mL). PMA (Fisher Scientific), cyclohexamide (Acros Organics), Gö6983 (Cayman Chemical), LY294002 (EMD Chemicals), UO126 (EMD Chemicals), BIM-1 (Cayman Chemical), Gö6976 (Enzo Life Sciences), AEB071 (Medchem Express), BAY 11-7082 (R&D Systems), MG132 (Fisher Scientific), and TPCA-1 (Cayman Chemical) were stored as recommended.
Leonard B., McCann J.L., Starrett G.J., Kosyakovsky L., Luengas E.M., Molan A.M., Burns M.B., McDougle R.M., Parker P.J., Brown W.L, & Harris R.S. (2015). The PKC-NFκB Signaling Pathway Induces APOBEC3B Expression in Multiple Human Cancers. Cancer research, 75(21), 4538-4547.
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6

Antagonist Screening for Cellular Signaling

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The antagonists used were as follows: 10 μm AMG9810 (Alomone, Jerusalem, Israel): a Trpv1 antagonist, 10 μm SB366791 (AdipoGen, San Diego, CA, USA): a Trpv1 antagonist, 1 μm Gö6983 (Cayman Chemical, Ann Arbor, MI, USA): a PKC antagonist, 100 nm staurosporine (LKT, St. Paul, MN, USA): a PKC antagonist, 5 μm calmidazolium (Cayman Chemical): a calmodulin antagonist, 10 μm KN93 (Cayman Chemical): a calmodulin kinase II antagonist, 3 μm KRIBB2 (Tocris Biosciences, Bristol, UK): a Hsf‐1 antagonist, 100 nm triptolide (Cayman Chemical): a Hsf‐1 antagonist, 50 μm VER155008 (Tocris Biosciences): a HSP70 antagonist, and 100 μm KNK437 (Tocris Biosciences): a HSP70 antagonist. All reagents were added into cells with a final concentration of 0.1% DMSO.
Obi S., Nakajima T., Hasegawa T., Nakamura F., Sakuma M., Toyoda S., Tei C, & Inoue T. (2018). Heat induces myogenic transcription factors of myoblast cells via transient receptor potential vanilloid 1 (Trpv1). FEBS Open Bio, 9(1), 101-113.
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7

Signaling Pathways in Cell Activation

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The following chemicals were obtained: Cyclosporin A (TCI Chemical cat no.C2408), Bay11–7082 (Enzo cat no.E1279), PP2 (Tocris cat no.1407), Gö 6983 (Cayman Chemical cat no.13311), INCB3284 (Cayman Chemical cat no.1163). 200,000 cells seeded in 24 well plates were treated with/without inhibitors. Protein cell lysates were prepared and immunoblotted using procedures described24 (link). Membranes were blotted with antibodies to: CD154 (Santa Cruz Biotechnology cat no. sc978), NFATc1 (Biolegend, cat no.649601), phospho-IKBa (ser32) (Cell Signaling Technology cat no. 9246), IΚBα or ACTIN (Sigma, cat no.A5441).
Brummer G., Fang W., Smart C., Zinda B., Alissa N., Berkland C., Miller D, & Cheng N. (2019). CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene, 39(11), 2275-2289.
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8

Receptor Binding and Interaction Assays

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IBOP ([1S-[1α,2α(Z),3β(1E,3S),4α]]-7-[3-[3-hydroxy-4-(4-iodophenoxy)-1-butenyl]-7-oxabicyclo[2.2.1]hept-2-yl]-5-heptenoic acid), SQ29548 ([(1S)1α,2β(5Z)3α,4β]-7-[3-[2-(phenylamino)carbonyl-hydrazino-methyl]-7-oxabicyclo-[2.2.1]-hept-2-yl]-5-heptenoic acid), and Gö6983 were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). AG1478 was from Calbiochem (San Diego, CA, USA). Bradykinin B-3259 (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg), Dimethyl pimelimidate dihydrochloride (DMP), and Duolink detection kit were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Dagher O.K., Jaffa M.A., Habib A., Ziyadeh F.N, & Jaffa A.A. (2019). Heteromerization fingerprints between bradykinin B2 and thromboxane TP receptors in native cells. PLoS ONE, 14(5), e0216908.
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