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Anti sp c

Manufactured by Abcam
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Anti-SP-C is a lab equipment product used for the detection and quantification of surfactant protein C (SP-C) in biological samples. It is a tool for researchers studying the role of SP-C in various biological processes and diseases.

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Lab products found in correlation

Anti ctbp1, by Abcam (2 mentions) Anti ctbp2, by Abcam (2 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Las 1000plus, by Fujifilm (1 mentions) Anti myc 9e10, by Santa Cruz Biotechnology (1 mentions) Anti β actin 2f3, by Fujifilm (1 mentions) Anti foxp2, by Merck Group (1 mentions) Anti erk2, by Santa Cruz Biotechnology (1 mentions) Anti sp b, by Santa Cruz Biotechnology (1 mentions) Anti vinculin, by Santa Cruz Biotechnology (1 mentions) Anti γ tubulin, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Rabbit anti-SP-C (3 citations)

4 protocols using anti sp c

1

Western Blot Immunoanalysis Protocol

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Immunoblotting analyses were carried out as described previously24 (link). Digital images were captured using the LAS-1000 Plus (Fujifilm). The following primary antibodies were used: mouse anti-Flag M2 (Sigma), anti-Myc 9E10 (Santa Cruz), and anti-β-actin 2F3 (Wako); rabbit anti-Myc and anti-SP-B (Santa Cruz), anti-CtBP1, anti-CtBP2, anti-SP-C, anti-Muc1 and anti-Abca3 (Abcam), anti-Foxp1 (CST), anti-Foxp2 (Sigma), anti-phospho-ERK1/2 (CST), and anti-ERK2 (Santa Cruz). An affinity-purified anti-MCRIP1 antibody was made in-house as described previously24 (link). All antibodies were used at a dilution of 1:1000 for western blotting. Full size western blot images are shown in Supplementary Fig. 6.
Weng J.S., Nakamura T., Moriizumi H., Takano H., Yao R, & Takekawa M. (2019). MCRIP1 promotes the expression of lung-surfactant proteins in mice by disrupting CtBP-mediated epigenetic gene silencing. Communications Biology, 2, 227.
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2

Neonatal Lung Protein Expression

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Tissues from neonatal lungs were isolated and samples were prepared for paraffin sections. Sections were stained with the following primary rabbit antibodies: anti-MCRIP1 (Atlas; 1:400); anti-SP-B (Santa Cruz; 1:300); anti-SP-C (Abcam; 1:1000), anti-CtBP1 (Abcam; 1:500) and anti-CtBP2 (Abcam; 1:500); anti-Foxp1 (CST; 1:200) and anti-Foxp2 (CST; 1:2000).
Weng J.S., Nakamura T., Moriizumi H., Takano H., Yao R, & Takekawa M. (2019). MCRIP1 promotes the expression of lung-surfactant proteins in mice by disrupting CtBP-mediated epigenetic gene silencing. Communications Biology, 2, 227.
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3

Protein Extraction and Western Blotting

Cited 1 time
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Protein extraction and western blotting experiments were performed as already described61 (link),62 . The antibodies used were: anti-β-actin (sc-1616, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), anti-γ-tubulin (sc-17787, Santa Cruz), anti-GAPDH (sc-32233, Santa Cruz), anti-vinculin (sc-7649, Santa Cruz), anti-PAX863 (link), anti-NKX2.133 (link), anti-FOXE164 (link), anti-SP-B (ab3430, Millipore), anti-SP-C (90716, Abcam), anti-HIPK2 (Novus Biologicals), anti-HMGA165 (link). Densitometric analyses of western blot were performed with Image J software.
Gerlini R., Amendola E., Conte A., Valente V., Tornincasa M., Credendino S.C., Cammarota F., Gentile C., Di Guida L., Paladino S., De Vita G., Fusco A, & Pierantoni G.M. (2019). Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death & Disease, 10(10), 747.
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4

Immunofluorescence Staining of Lung Specimens

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The protein expression in lung specimens was also evaluated by immunofluorescence staining on fixed frozen sections similar to HE staining as previously described [7 (link)]. Brain sliced sections on coverslips were fixed in 4% paraformaldehyde. Samples were blocked by 5% donkey serum prior to incubation with primary antibody overnight with the following antibodies: anti–SP-C (Abcam, Cambridge, MA) at 4 °C overnight, followed by fluorescein-conjugated antibodies for immunofluorescence (Beyotime Biotechnology, Shanghai, China). Then, the sliced sections were placed on glass slides for laser-confocal microscope observation.
Pan P., Qu J., Li Q., Li R., Yang Y., Zuo S., Liu X., Feng H, & Chen Y. (2020). Aggravated pulmonary injury after subarachnoid hemorrhage in PDGF-Bret/ret mice. Chinese Neurosurgical Journal, 6, 13.
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