HOS and MG63 cells overexpressing miR-210-5p or negative control were fixed with 1% formaldehyde, followed by chromatin fragmentation, lysed in NETN buffer, and then incubated with Dynabeads Protein A (Invitrogen) supplemented with clone 2A8 antibody (Millipore), anti-Pan-Ago, or IgG control for immunoprecipitation. The immunoprecipitated RNA was released by proteinase K digestion, and extracted by phenol/chloroform/isopropyl alcohol. RNA was isolated by glycogen ethanol precipitation and treated with DNase I.
Clone 2a8 antibody
Manufactured by Merck Group
The Clone 2A8 antibody is a laboratory tool used for scientific research. It is a monoclonal antibody that can be used to detect and quantify the presence of a specific target molecule in a sample.
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Lab products found in correlation
4 protocols using clone 2a8 antibody
1
Chromatin Immunoprecipitation of miR-210-5p
2
Microglia miRNA Enrichment Analysis
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Microglia overexpressing miR-124-3p or miR-NCOE were fixed with 1% formaldehyde followed by chromatin fragmentation, lysed in the NETN buffer, and incubated with Dynabeads Protein A (Invitrogen) supplemented with clone 2A8 antibody (Millipore), anti-Pan-Ago, or IgG control for immunoprecipitation. Proteinase K digestion and phenol/chloroform/isopropyl alcohol were used to release and extract the immunoprecipitated RNA. RNA was isolated by glycogen ethanol precipitation and then treated with DNase I.
3
miR-455-3p Colon Cancer Immunoprecipitation
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Colon adenocarcinoma cells that overexpressed miR‐455‐3p were fixed with 1% formaldehyde. The cells were lysed using NETN buffer and cultured with Dynabeads protein A (Invitrogen) supplemented with IgG or anti‐Pan‐Ago and clone 2A8 antibody (Millipore). Proteinase K digestion was used to release immunoprecipitated RNA. RNA was extracted, purified by ethanol precipitation with glycogen and treated with DNase I.
4
Profiling miRNA-Protein Interactions in BV2 Microglia
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BV2 microglia overexpression miR-216a-5p or miR-NCOE were fixed with 1% formaldehyde, followed by chromatin fragmentation, lysed in NETN buffer and then incubated with Dynabeads Protein A (Invitrogen) supplemented with clone 2A8 antibody (Millipore), anti-Pan-Ago, or IgG control for immunoprecipitation. The immunoprecipitated RNA was released by proteinase K digestion and extracted by phenol/chloroform/isopropyl alcohol. RNA was isolated by glycogen ethanol precipitation and treated with DNase I.
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