RNAi-mediated gene knockdown was done with the 19-nucleotide targets using siRNAs (GenePharma, Shanghai, China) as follows: Lon siRNA sense: 5′-GGAGCAGCUAAAGAUCAUCTT-3′ Lon siRNA antisense: 5′-GAUGAUCUUUAGCUGCUCC TG-3′ Control siRNA sense: 5′-UUCUCCGAACGUGUCACGUTT-3′ Control siRNA antisense: 5′- ACGUGACACGUUCGGAGAATT-3′ UM-UC-3 and SCaBER cells (2×105) were transfected with a final concentration of 100 nmol/L siRNAs using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to manufacturer's instructions.
Control sirna
Manufactured by Thermo Fisher Scientific
Sourced in United States, China, France, United Kingdom
Control siRNA is a laboratory reagent used in RNA interference (RNAi) experiments. It serves as a negative control, allowing researchers to assess the specificity of gene silencing effects observed with experimental siRNA molecules.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (104 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (67 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (32 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (27 mentions)
Opti mem, by Thermo Fisher Scientific (25 mentions)
Control sirna, by GenePharma (24 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (23 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (20 mentions)
Rnaimax, by Thermo Fisher Scientific (18 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (18 mentions)
Control sirna, by Santa Cruz Biotechnology (18 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (15 mentions)
Sirnas, by Thermo Fisher Scientific (10 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions)
Trizol, by Thermo Fisher Scientific (9 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (8 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (8 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions)
Hiperfect transfection reagent, by Qiagen (7 mentions)
Opti mem medium, by Thermo Fisher Scientific (6 mentions)
462 protocols using control sirna
1
RNAi-Mediated Lon Knockdown in Bladder Cancer
2
Targeting EMT Regulators in GBM
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Human GBM-derived ECs were transfected with siRNAs targeting Twist1 (Thermo Fisher Scientific, s14523), Snail (Thermo Fisher Scientific, s13185), Satb1 (Thermo Fisher Scientific, 106912), or control siRNA (Thermo Fisher Scientific, 4390843) using Lipofectamine 2000 Transfection Reagent (Life Technologies, 11668-019) in serum-free Opti-MEM medium (Gibco, 31985-070) for 12 hours, followed by incubation with serum-supplemented medium for 48 hours. For in vivo treatment, cells were incubated with LNPs containing Twist1 siRNA (Thermo Fisher Scientific, 69856) or control siRNA (Thermo Fisher Scientific, 4404020).
3
Silencing NNT-AS1 in Colorectal Cancer
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Silencer select small interference RNAs (siRNAs) specific to NNT-AS1 (siRNA IDs s501613 and s501614) and a control siRNA (#4390843) were obtained from Ambion (Invitrogen, Carlsbad, CA, USA). For silencing NNT-AS1 in CRC cells, the NNT-AS1-specific RNAs (5’-GAAAAGAAAAAGAAGCUUAtt-3’; 5’-GCAACA GAGUGAUACUCUAtt-3’) and control siRNA (5’- TTCTCCGAACGTGTCACGT-3’) were transfected into SW480 and SW620 cells using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) and Opti-MEM (Gibco, Carlsbad, CA, USA) according to the manufacturer's recommendations.
4
Efficient siRNA Knockdown in Cells
5
Silencing Nrf2 in Primary Chondrocytes
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We obtained siNrf2 and control siRNA from Applied Biosystems (Thermo Fisher Pierce, Waltham, MA, USA). Primary chondrocyte cells were seeded in 6-well plates and transfected with siNrf2 or control siRNA using lipofectamine 2000 reagent (Invitrogen, Waltham, MA, USA) in accordance with the manufacturer’s instructions. The cells were then cultured for 24 h prior to use in the experiments.
6
Silencing ERβ and ATG7 in SH-SY5Y cells
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ERβ silencing siRNA, ATG7 silencing siRNA, and control siRNA were purchased from GenePharma (Suzhou, China). The sequences of ERβ, ATG7, and control siRNA were as follows: ERβ sense, 5′-CCAGCCAUGACAUUCUAUATT-3′ and antisense, 5′-UAUAGAAUGUCAUGGCUGGTT-3′; ATG7#1 sense, 5′-GGUCAAAGGACG-AAGAUAATT-3′ and antisense, 5′-UUAUCUUCGUCCUUUGACCTT-3′; ATG7#2 sense, 5′-GCCUCUCUAUGAGUUUGAATT-3′ and antisense, 5′-UUCAAACUCA-UAGAGAGGCTT-3; control siRNA sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense, 5′-ACGUGACACGUUCGGAGAATT-3′. These kinds of siRNA oligonucleotides were transfected into SH-SY5Y cells at 100 nM using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s instructions.
7
PAR-2 Silencing in Endothelial Progenitor Cells
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PAR-2 siRNA and control siRNA (scrambled siRNA) were purchased from Invitrogen (Carlsbad, CA, USA). PAR-2 siRNA and control siRNA were transfected into EPCs to a final concentration of 5 nM with 0.3% Lipofectamine RNAiMax (Invitrogen). Transfected cells were incubated at 37 °C in an atmosphere containing 5% CO2 for 7 days before use in experiments.
8
Silencing VEGF-C and Akt for Cell Studies
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Small interfering RNA (siRNA) duplexes specific for VEGF-C or Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, Calif.); control siRNAs with sequences that do not target any gene product were obtained from Invitrogen (Carlsbad, Calif.). Cells were transfected with 25 nM VEGF-C siRNA, Akt siRNA, or control siRNA in serum-free Opti-MEM using the oligofectamine method (Invitrogen) for 1 h at 37 °C. After changing the culture medium, cells were cultured for 24 h at 37 °C prior to further experiments.
9
Regulation of Esophageal Cancer Cells
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Two esophageal cancer cell lines (EC109 and KYSE150) and a human esophageal epithelial cell line (HET-1A) were purchased from the Chinese Academy of Sciences (Shanghai, China). All the cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO2.
The Rab10 small interfering RNAs (siRNAs), the control siRNA, miR-378a-3p mimics, miR-378a-3p inhibitors and the corresponding negative control (NC) were purchased from GenePharma Company (Shanghai, China). siRNA target sequences were as follows: Rab10 siRNA, 5′-GGG GTA ATG CAG AAG TGA T-3′ and control siRNA, 5′-GCA TCA TGA TAG TGT ATG A-3′. The cells were seeded at 3×105 cells per well in a 6-well plate and transfected with either Rab10 siRNA, the control siRNA, the miR-378a-3p mimics, the miR-378a-3p inhibitors, or the corresponding NC at a final concentration of 50 nM using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's protocols.
The Rab10 small interfering RNAs (siRNAs), the control siRNA, miR-378a-3p mimics, miR-378a-3p inhibitors and the corresponding negative control (NC) were purchased from GenePharma Company (Shanghai, China). siRNA target sequences were as follows: Rab10 siRNA, 5′-GGG GTA ATG CAG AAG TGA T-3′ and control siRNA, 5′-GCA TCA TGA TAG TGT ATG A-3′. The cells were seeded at 3×105 cells per well in a 6-well plate and transfected with either Rab10 siRNA, the control siRNA, the miR-378a-3p mimics, the miR-378a-3p inhibitors, or the corresponding NC at a final concentration of 50 nM using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer's protocols.
10
Sirt2 Knockdown in Neuronal Cells
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Sirt2 knockdown was performed using predesigned Stealth siRNA against Sirt2 (Sirt2 siRNA), and a Stealth siRNA negative control was used as control siRNA (Invitrogen). The Sirt2 siRNA sequences were as follows: sense, 5′-GCCAUCUUUGAGAUCAGCUACUUCA-3′; antisense, 5′-UGAAGUAGCUGA-UCUCAAAGAUGGC-3′. Primary cultured neurons, Neuro2a, and Neuro2a-P301L cells were transiently transfected with Sirt2 or control siRNA using Lipofectamine RNAiMAX (Invitrogen) in accordance with the manufacturer’s instructions. Seventy-two hours after transfection, the cells were harvested to detect Sirt2 knockdown by Western blotting.
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