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4 protocols using quercetin 3 4 diglucoside

1

Phytochemical Analysis of Natural Antioxidants

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Sigma-Aldrich (St. Louis, MO, USA) provided 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2′,7′-dichlorofluorescein diacetate (DCF-DA), lipopolysaccharide (LPS), Folin–Ciocalteu (F–C) reagent, gallic acid, AlCl3, Griess reagent, NG-Methyl-L-arginine acetate salt (L-NMMA), WY14643, CaCl2, and HPLC grade standards of quercetin, kaempferol, isorhamnetin, kaempferol 3-glucoside, isorhamnetin 3-glucoside, quercetin 3-glucoside, and quercetin 3,4′-diglucoside. Solvents for extraction and fractionation (ethanol, n-hexane, chloroform, ethyl acetate, and n-butanol) and formic acid were purchased from Daejung Chemicals & Metals (Siheung, Republic of Korea). Solvents for LC/MS (methanol, acetonitrile) were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA).
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2

Quantitative Analysis of Quercetin Glycosides

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Standard compounds such as quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside were purchased from Sigma–Aldrich, Korea. The flavonoid standards were dissolved in 80% MeOH at several concentrations and a high linearity was obtained from each curve. quercetin, quercetin-3,4′-diglucoside and quercetin-4′-glucoside were identified based on their retention times and their concentrations were calculated by comparing the peak areas of samples with standards and correlated with obtained results.
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3

Antioxidant Profile of Red Onion Cultivars

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Honeysuckle red onions from J & D Produce, Inc., Texas and Artisan sweet Italian red onions from Tanimura & Antle, California were used for the entire study (Figure 1). Sodium pyruvate, 2, 4-dinitrophenylhydrazine (DNPH), glucose, sucrose, fructose, quercetin 3-glucoside, quercetin-3,4′-diglucoside, quercetin, delphinidin 3-glucoside, L-ascorbic acid, gallic acid, dextrose, sodium carbonate, 2,2-diphenyl-1-picryhydrazyl, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid), Folin-Ciocalteu reagent, HPLC grade methanol, acetonitrile, formic acid, and metaphosphoric acid were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium hydroxide (NaOH) was obtained from Fisher Scientific (Pittsburg, PA, USA).
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4

Analytical Reagents for Polyphenol Analysis

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Methanol (HPLC-grade) was supplied by Lab-Scan (Dublin, Ireland). Folin-Ciocalteu´s phenol reagent, ferulic acid, gallic acid, quercetin, quercetin-3-glucoside, quercetin-3,4′-diglucoside and isorhamnetin-3-glucoside were purchased from Sigma-Aldrich (St Louis, MO, USA).
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