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Il 1β elisa kit

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The IL-1β ELISA kit is a laboratory assay used to quantify the levels of the cytokine interleukin-1 beta (IL-1β) in biological samples. This kit employs the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure IL-1β concentrations.

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Lab products found in correlation

Tnf α elisa kit, by MultiSciences Biotech (3 mentions) Il 6 elisa kit, by MultiSciences Biotech (3 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Lipopolysaccharide lps, by Cell Signaling Technology (1 mentions) Milli q system, by Merck Group (1 mentions) Trypsin, by Beyotime (1 mentions) Ldh assay kit, by Nanjing Jiancheng (1 mentions) Dulbecco s modified eagle medium dmem, by Keygen Biotech (1 mentions) Tnf α elisa kit, by Thermo Fisher Scientific (1 mentions) Il 6 elisa kit, by Neobioscience (1 mentions) Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Enzyme labeled instrument, by Thermo Fisher Scientific (1 mentions) Annexin 5 fitc pi kit, by Biosharp (1 mentions) Mangiferin, by Solarbio (1 mentions) Anti vegf, by Affinity Biosciences (1 mentions) Cck 8 kit, by Solarbio (1 mentions) Anti lc3, by Proteintech (1 mentions) Nf κb p65 d14e12 rabbit mab, by Cell Signaling Technology (1 mentions) Iκbα 44d4 rabbit mab, by Cell Signaling Technology (1 mentions) H e staining solution, by Nanjing Jiancheng (1 mentions)

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IL-1β ELISAs

9 protocols using il 1β elisa kit

1

Salvianolic Acid B Attenuates Inflammatory Responses

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Salvianolic acid B (Sal B) was provided by Nanjing Hongqiao Institute of Pharmaceutical Technology. Dulbecco’s modified eagle medium (DMEM) was purchased from KeyGEN BioTECH Co., Ltd. (Nanjing, China). Fetal bovine serum was purchased from Biological Industries Co., Ltd. (Israel). Trypsin was purchased from Beyotime Institute of Biotechnology Co., Ltd. (Shanghai, China). LDH assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). IL-1β ELISA kit was purchased from multi sciences Co., Ltd. (Hangzhou, China). cTn ELISA kit was purchased from YIFEIXUE BIO TECH (Nanjing, China). Evagreen 2x qPCR MasterMix-Low Rox kit was purchased from Abmgoodchina Inc (Zhenjiang, China). Lipopolysaccharide (LPS) was purchased from Cell Signaling Technology, Inc (USA). Adenosine triphosphate disodium (ATP) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Primary antibodies against Myd88/IRAK1/NF-κB/NLRP3/β-actin were obtained from proteintech group, Inc (Wuhan, China). Primary antibodies against MD-2 were obtained from Bioss Biotechnology Co., Ltd. (Beijing, China). Antibody for TLR4 was obtained from Santa Cruz Biotechnology (USA). Water used in this study was purified by a Milli-Q system (Millipore, MA, USA). All culture plates were obtained from corning (Corning, USA).
Hu Y., Li Q., Pan Y, & Xu L. (2019). Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome. Molecules, 24(23), 4416.
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2

Quantifying Inflammatory Cytokines in Spinal Cord

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L4–L6 segmental spinal cord tissue was grounded, and the slurry was centrifuged at 3000 rpm at 4°C for 10 min. The supernatants were collected as protein samples. The levels of TNF-α and IL-1β were measured using a TNF-α ELISA kit (Thermo Fisher Scientific, Waltham, MA, USA) and an IL-1β ELISA kit (MultiSciences, Hangzhou, China), respectively, per the manufacturers' instructions.
Deng Y., Yang L., Xie Q., Yang F., Li G., Zhang G., Li S., Wu Z., Wang J, & Kang X. (2020). Protein Kinase A Is Involved in Neuropathic Pain by Activating the p38MAPK Pathway to Mediate Spinal Cord Cell Apoptosis. Mediators of Inflammation, 2020, 6420425.
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3

Mouse Serum Cytokine Detection

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Mouse serum cytokines were detected by the IL-1β ELISA Kit (70-EK201B, MultiSciences, Hangzhou, China) and the IL-6 ELISA Kit (EMC004.96, NeoBioscience, Shenzhen, China). Blood acquired from mice was centrifuged at 3000× g, 4 °C, and the supernatant was purified for ELISA assay according to the manufacturer’s instructions. Optical density was measured at a wavelength of 450 nm.
Lin J., Ling Q., Yan L., Chen B., Wang F., Qian Y., Gao Y., Wang Q., Wu H., Sun X., Shi Y, & Kong X. (2022). Ancient Herbal Formula Mahuang Lianqiao Chixiaodou Decoction Protects Acute and Acute-on-Chronic Liver Failure via Inhibiting von Willebrand Factor Signaling. Cells, 11(21), 3368.
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4

Quantification of Inflammatory Cytokines

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Concentrations of interleukin (IL) -6 and IL-1β in the supernatant of cell co-culture medium or tendon tissue were measured using an IL-6 ELISA kit (Thermo Fisher) and IL-1β ELISA kit (Multi Sciences, Hangzhou, China), respectively. Briefly, the supernatant samples were added to the wells in triplicate and incubated for 2 h at 37 °C. Then, they were incubated in an HRP conjugate solution for 30 min at 37 °C and TMB solution for 30 min at 22–26 °C. ELISA plates were detected at 450 nm using an FLx800TM Fluorescence microplate reader (BioTek, USA). Quantitative data are presented as average concentrations in pg/mL.
Wei B., Ji M., Lin Y., Wang S., Liu Y., Geng R., Hu X., Xu L., Li Z., Zhang W, & Lu J. (2023). Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo. Stem Cell Research & Therapy, 14, 104.
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5

Quantifying Inflammatory Cytokines in Mice

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To measure the circulating TNF-α, IL-6, and IL-1β levels in vivo, blood was removed from the eyes. The samples were stored frozen before sample testing. The levels of TNF-α, IL-6, and IL-1β in the murine serum samples were analyzed using TNF-α ELISA kit (70-EK282HS-96; MULTI SCIENCES), IL-6 ELISA kit (70-EK206HS-96; MULTI SCIENCES), and IL-1β ELISA kit (EK201B/3-96; MULTI SCIENCES) according to the manufacturer’s instructions.
Zhao J., Chen S., Zhu L., Zhang L., Liu J., Xu D., Tian G, & Jiang T. (2021). Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer. Frontiers in Oncology, 10, 621092.
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6

Quantifying Hippocampal IL-1β Levels

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The levels of IL-1β in the hippocampal tissues or culture supernatant of hippocampal neurones were assessed using IL-1β ELISA Kit (MultiSciences, Hangzhou, China) according to the manufacturers' instruction. The optical density values of samples were detected using enzyme-labeled instrument (Thermo Fisher Scientific, Waltham, MA, USA).
Gu X., Wu H., Xie Y., Xu L., Liu X, & Wang W. (2021). Caspase-1/IL-1β represses membrane transport of GluA1 by inhibiting the interaction between Stargazin and GluA1 in Alzheimer’s disease. Molecular Medicine, 27, 8.
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7

Alzheimer's Disease Pathogenesis Study

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All drugs, kits, and antibodies used in this study were purchased as listed: mangiferin, Solarbio, China, IM0030; CCK-8 kit, Solarbio, CA1210; IL-1β ELISA kit, Multisciences, China, EK201B; IL-6 ELISA kit, Multisciences, EK2153; TNFα ELISA kit, Multisciences, EK282; Annexin V-FITC/PI kit, BioSharp, China, BL110A; anti-LC3, Proteintech, China, 14600-1-AP; anti-Aβ42, Bioss, USA, BS0107R; Anti-p-Tau, Affinity, USA, AF3148; Anti-APJ, Affinity, DF13350; Anti-VEGF, Affinity, AF5131; Anti-S100β, Affinity, DF6116; Anti-NSE, Affinity, AF5473.
Tan H., Liang D., Lu N., Zhang J., Zhang S, & Tan G. (2024). Mangiferin attenuates lipopolysaccharide-induced neuronal injuries in primary cultured hippocampal neurons. Aging, 16(10).
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8

Molecular Pathways Analysis via ELISA and Immunoblotting

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H&E staining solution was purchased from Nanjing Jiancheng Bioengineering Institute. The Rat IL-17 ELISA Kit, TNF-α ELISA Kit, IL-6 ELISA Kit, and IL-1β ELISA kit were purchased from MultiSciences (Lianke) Biotech Co. Ltd. (Hangzhou, China). The IκBα (44D4) Rabbit mAb, NF-κB p65 (D14E12) Rabbit mAb, and Phospho-IKKα/β (ser176/180) (16A6) Rabbit mAb were purchased from Cell Signaling Technology, Inc. (USA). The 5% TNBS solution was purchased from Sigma Co. (USA). Reference compounds including Campanulin, Isoflavone, Anthocyanins, Miscanthus, ferulic acid, astragaloside A, quercetin, coniferyl ferulate, Senkyunolide I, Senkyunolide A, z-ligustilide were purchased from Chengdu Pusi Biological Polytron Technologies Inc. (Chengdu, China). The purity of all the reference compounds was identified after being purchases.
Hu Z., Yang M., Ye Q., Qin K., Wu M., Gu R, & Zhou Y. (2018). Tou Nong San Attenuates Inflammation in TNBS-IBD Model by Inhibiting NF-κB Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2018, 6929307.
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9

Serum Biomarker Quantification Protocol

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Serum and cell supernatants were collected and CK-MB, LDH, IL-1βand IL-18 levels in serum and cell supernatants according to instructions. The CK-MB and LDH kits were purchased from Nanjing Jiancheng Bioengineering Institute, China. The IL-1βELISA kit and the IL-18 ELISA kit were purchased from MULTISCIENCES (LIANKE) BIOTECH, CO., LTD
Li w., Shen X., Feng S., Liu Y., Hu R., Zhou G., Sang M., Sun X., Jiao R., & Liu F. (2022). BRD4 inhibition by JQ1 protects against LPS-Induced Cardiac Dysfunction by inhibiting SIRT1-dependent activation of NLRP3 inflammasomes.
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