US-guided RFA was performed or supervised by an experienced hepatologist (T.Y. Lee) whose cumulative operator experience was more than 400 cases [21 (link)], mainly with a commercially available RFA system (Cool-tip; Valley Lab, Boulder, CO). After intravenous sedation, analgesia and local anesthesia were administered, the RFA needle was inserted through real-time guidance of US (Aplio™ 300, Toshiba medical systems cooperation). For better tumor approach or vital organ protection, artificial ascites or artificial pleural effusion might be performed before the RFA procedure. In addition, the needle placement could be determined by the operator to create overlapping ablation zones. After completion of the RFA procedure, a larger hyperechoic area with safe margin completely covered the ablated tumor. Repeat ultrasound was performed the day after treatment for evaluation of the preliminary treatment results and complications. Dynamic CT or MRI was routinely arranged one month later to evaluate technique efficacy [22 (link)]. If any residual tumors were found, another RFA session would be arranged.
Aplio 300
Manufactured by Toshiba
Sourced in Japan
The Toshiba Aplio 300 is a medical imaging device designed for ultrasound imaging. It provides high-quality digital imaging capabilities for various diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Acuson s2000, by Philips (1 mentions)
Somatom definition as, by Siemens (1 mentions)
Agarose, by Promega (1 mentions)
Slacker, by Smooth-On (1 mentions)
Graphite, by Merck Group (1 mentions)
Spss statistic 27, by IBM (1 mentions)
Tm 3000 tabletop sem, by Hitachi (1 mentions)
Phillips iu22, by Philips (1 mentions)
Ascendus, by Hitachi (1 mentions)
Aplio 500, by Toshiba (1 mentions)
Aplio 400, by Toshiba (1 mentions)
Selenia dimension, by Hologic (1 mentions)
29 protocols using aplio 300
1
Ultrasound-Guided Radio-Frequency Ablation for Liver Tumors
2
Echocardiographic Assessment of Cardiac Function
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Transthoracic echocardiography was performed to evaluate cardiac function using the Aplio 300 (Toshiba Medical System) and a 14-MHz transducer. Mice were initially anesthetized with 2% isoflurane, reduced to 1% during examinations. Left ventricular short-axis dimensions at the tip of the papillary muscles were measured on M-mode. Fractional shortening was calculated as (LVDd - LVDs)/LVDd × 100 (%).
3
Evaluating Cardiac Function in Mice
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Transthoracic electrocardiography (ECG) was used to evaluate cardiac function with the Aplio 300 (Toshiba Medical System) and a 14-MHz transducer. The 10-week-old male mice were anesthetized initially with 2% isoflurane, and then at 1% during the examination. Left ventricular short-axis dimensions at the tip of the papillary muscles were measured on M-mode. Fractional shortening was calculated as (LVDd–LVDs)/LVDd × 100 (%).
4
Adnexal Lesions Diagnostic Imaging Protocol
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All the examinations were performed by five gynecologists with at least 7 yearsof experience in TVUS on the same unit (Aplio 300, Toshiba Medical Systems, Tokyo, Japan), using a dedicated endovaginal probe (4–10 MHz). Each of the selected examinations were comprised of several images, but only conventional B-mode images were selected. Images were retrieved in digital imaging and communications in medicine (DICOM) format and imported into a dedicated radiology workstation (General Electric, Advantage workstation, 4.7 edition, Waukesha, WI, USA). Images were reviewed by two researchers (one gynecologist and one radiologist, M.D.O. and C.M.M.), who were aware of the subjects’ final diagnostics, pathological findings, and clinical outcomes. When multiple adnexal lesions were observed on the same patient, the TVUS examinations were cross-referenced with the medical data to ensure the selection of only the lesions that were previously documented. Respective lesions were marked, and for every lesion, one image was anonymized and retrieved for subsequent analysis.
5
Comprehensive Echocardiographic Assessment
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Echocardiographic examination was performed using a commercially available system (Aplio 300, Toshiba Medical Systems, Tokyo, Japan) in accordance with a standardized protocol by trained sonographers blinded to the participant's clinical information. The dimensions of the cardiac chambers were measured in the standard manner.21 LV mass was calculated with a validated formula22 : LV mass = 0.8{1.04[(SWT + LVEDD + PWT)3−LVEDD3]} + 0.6, where SWT is the LV end‐diastolic septal wall thickness, LVEDD is the LV end‐diastolic diameter, and PWT is the LV end‐diastolic posterior wall thickness. Left atrial volume was measured from the apical two‐chamber and four‐chamber views using the biplane Simpson's rule.21 LV mass and left atrial volume were then indexed for body surface area. LV diastolic parameters were assessed according to the current guideline.23 Briefly, transmitral diastolic flow was obtained from an apical four‐chamber view. Pulsed‐wave Doppler examination of mitral inflow was performed to measure early (E) and late peak velocity. Peak early diastolic mitral annular velocity (e′) was also measured from tissue Doppler imaging in the lateral and the septal mitral annulus, and the average value was used. The ratio of E to mean e′ was then calculated (E/e′).
6
Multimodal Liver Imaging Cohort
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We prospectively collected this cohort (n = 246) from patients that had both an US and a FibroScan study ordered and if D.I.T., Y.C.C., T.H.H., or C.J.C. conducted the image study. With the agreement of these patients, they were scanned by Siemens Acuson S2000, Philips IU22, and Toshiba Aplio 300 scanners on the same day. Studies were collected over a period from August 30, 2018 to August 27, 2019, and we only included patients diagnosed with the HBV, HCV, and NBNC criteria used in HP-U. The tri-machine (TM) cohort allowed for an assessment of agreement across scanners.
7
Ultrasound Imaging of Anatomical Structures
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Toshiba APLIO 300 ultrasound machine with a standard convex transducer of 2.5 – 3.5 MHz was used to take all measurements.
8
Ultrasound and Elastography for Lesion Evaluation
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Ultrasound and elastography examinations were performed by the same radiologist with 3 years of experience using elastography. The device used was a Toshiba Aplio 300 (Toshiba, Tokyo, Japan), and the data were analyzed using the commercial software for SE available on the device. Before the biopsy, elastography images were acquired using the technique proposed in previous studies.13 (link) The images that best represented the lesion using conventional US and SE were chosen. The selected images were stored in the picture archiving and communication system (PACS) consecutively without any identification of the nature of the lesions.
9
Evaluating RA Disease Activity with MMP-3 Ratio
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The disease activity of RA was assessed by examining CRP, ESR, the number of tender and swollen joints, patient’s global assessment, evaluator’s global assessment, DAS28ESR, CDAI, and US (via an Aplio 300 with PLT-1204BT transducer, TOSHIBA Medical Systems Corporation, Tochigi, Japan) at baseline and at 12 and 24 weeks. Clinical assessment was performed by two different rheumatologists blinded to the US findings [9 ].
According to the values of DAS28ESR at 24 weeks, the patients were divided into the DAS remission group (DAS28ESR <2.6) and the DAS non-remission group (DAS28ESR ≥2.6). Similarly, according to the results of US examination, the patients were divided into the US remission group (total PD score <3) and the US non-remission group (total PD score ≥3) [12 ]. The MMP-3 ratio was compared between these groups. We also determined the optimal cutoff values of the MMP-3 ratio.
According to the values of DAS28ESR at 24 weeks, the patients were divided into the DAS remission group (DAS28ESR <2.6) and the DAS non-remission group (DAS28ESR ≥2.6). Similarly, according to the results of US examination, the patients were divided into the US remission group (total PD score <3) and the US non-remission group (total PD score ≥3) [12 ]. The MMP-3 ratio was compared between these groups. We also determined the optimal cutoff values of the MMP-3 ratio.
10
Evaluating Microvascular Anastomosis Patency
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Perfusion and patency of the vessels that were anastomosed using the T-shaped metal stents were assessed using ultrasonogra-phy (Aplio 300; Toshiba, Tochigi, Japan) or contrast-enhanced computed tomography (SOMATOM Definition AS; Siemens, Tokyo, Japan). In 3 cases, blood flow was evaluated using ultraso-nography at 4 weeks after the microvascular anastomosis. Contrast-enhanced computed tomography was performed in 1 case, and this subject was prepared for the examination using this modality.
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