Xterra ms

Manufactured by Waters Corporation

Sourced in United States

The XTerra MS is a liquid chromatography-mass spectrometry (LC-MS) system developed by Waters Corporation. It is designed for analytical separation and detection of a wide range of chemical compounds. The system combines a high-performance liquid chromatography (HPLC) module with a mass spectrometer to enable efficient and sensitive analysis of complex samples.

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Lab products found in correlation

Symmetry c18, by Waters Corporation (2 mentions) Maxis q tof, by Bruker (1 mentions) Avance 3 600 nmr spectrometer, by Bruker (1 mentions) Ultimate 3000, by Bruker (1 mentions) Spin x, by Corning (1 mentions) Guard cartridge, by Shiseido (1 mentions) Capcell c18 mg s 5, by Shiseido (1 mentions) Api 3000 triple quadrupole mass spectrometer, by AB Sciex (1 mentions)

7 protocols using xterra ms

Flavonoid Profiling of Astragalus microcephalus

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Astragalus microcephalus was dissolved with the minimum amount of water and then filtrated with a 0.45 μm pore size filter. 10 μL of the extract was injected into Agilent 6100 Quadrupole LC/MS system to detect flavonoids, which were checked with library standard chromatogram in the system using the diode array detector SL (micro flow cell: 2 μL, 3 mm path length).
LC/MS was conducted according to the Lin method [21 (link)]. For this purpose, an Agilent series 6100 LC/MS system (Agilent Technologies, Santa Clara, USA) with a photodiode array detector was set at 260 nm. The UV spectra were examined at 200–500 nm to obtain the maximum absorbance wavelength. A 150 × 3.0 mm, 3.5 μm Waters XTerra MS with a sentry guard column (Symmetry C18, 5 μm, 20 × 3.9 mm) was used. The mobile phase contained (1) water with 0.25% (v/v) acetic acid and (2) acetonitrile with 0.25% (v/v) acetic acid using a linear gradient of 17%–42% (v/v) (2) for 38 min. The temperature and flow rates were set to 45°C and 0.2 ml/min, respectively.
The LC system was joined to the mass spectrometer directly without stream splitting and using an electrospray interface Model HP 59987A. The ESI-MS spectra were obtained from the positive ion mode. The nebulizer pressure (N2) was 5.5 × 105 Pa, and the temperature of the dying gas (N2) was set to 350°C, with a gas flow rate of 40 ml/min.

Akbari F., Azadbakht M., Bagheri A, & Vahedi L. (2022). In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd.. Advances in Pharmacological and Pharmaceutical Sciences, 2022, 2156629.

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LC/MS Analysis of Atrifil Extract and Oshagh Gum

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The Atrifil extract and Oshagh gum LC/MS was conducted according to the Herbani method [56 ]. For this purpose, an Agilent series 6100 LC/MS system (Agilent Technologies, Santa Clara, CA, USA) with a photodiode array detector was set at 257 nm. A 150 mm × 3.0 mm, 3.5 μm Waters XTerra MS with symmetry C₁₈ (5 μm, 20 × 3.9 mm) was used. The mobile phase was consisted of (1) water with 0.1% (v/v) formic acid and (2) acetonitrile with 0.1% (v/v) formic acid using gradient solvent system of 10–90% (v/v) (2) for 26 minutes. The temperature was 35°C, and flow rate was 0.3 ml/min.
The mass spectrometer is directly joined to the LC system without stream splitting and by using an electrospray interface Model HP 59987A. The nebulizer pressure, temperature of drying gas (N2), and gas flow rate were 5.5 × 10⁵ Pa, 300°C, and 40 ml/min, respectively.

Akbari F., Azadbakht M., Gaurav A., Azimi F., Mahdizadeh Z., Vahedi L., Barzegar Nejad A., Chabra A, & Eghbali M. (2022). Evaluation of the Therapeutic Effect of the Traditional Herbal Medicine Atrifil and Oshagh Gum on Testosterone-Induced Benign Prostatic Hyperplasia in Wistar Rats. Advances in Urology, 2022, 5742431.

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Structural Elucidation of Compounds

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The structures were elucidated based on ESI-MS, 1D, and 2D NMR. NMR spectra were recorded on a Bruker Avance III-600 NMR spectrometer (Bruker, Billerica, MA, USA) with TMS as an internal standard. Mass spectrometry analysis was performed using an XTerraMS (Waters, Milford, MA, USA) equipped with an electrospray ionization (ESI) source. HR-ESI-MS data were acquired in m/z by using a BioTOF^TM-Q mass spectrometer (Bruker, Billerica, MA, USA) and a Dionex Ultimate 3000 coupled to a Bruker Maxis Q-TOF.

Zhang W., Zhao B., Du L, & Shen Y. (2017). Cytotoxic Polyketides with an Oxygen-Bridged Cyclooctadiene Core Skeleton from the Mangrove Endophytic Fungus Phomosis sp. A818. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(9), 1547.

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GABA Quantification in Dorsolateral Striatum

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The dorsolateral striatum from both the left and right sides (n = 10, per group) was dissected and frozen at −80°C. Brain tissue samples were weighed and then homogenized in 700 μL of ice-cold lysis buffer [19 (link)], containing o-phthalaldehyde 27 mg, anhydrous ethanol 1 mL, tetraborate buffer 9 mL, andβ-mercaptoethanol 5 μL.
The homogenate was centrifuged at 14000 rpm for 15 min at 4°C and filtered through a 0.22 μm filter (Costar, Spin-X) and then centrifuged at 7000 rpm. Standard solution or the filtrate obtained from brain homogenate (20 μL) was injected into the HPLC. Chromatographic conditions were as follows. The precolumn was Shiseido (Guard Cartridge, Capcell C18 MG S-5, 4.0 × 10 mm). The chromatographic column was Waters XTerra MS (3.0 × 50 mm, 2.5 um, Part no. 186000598). The mobile phase was composed of 100 mM disodium hydrogen phosphate, 25% methanol, and 10% acetonitrile (pH 6.70). The flow rate was 0.6 mL/min. The column oven was held constant at 40°C. Working solutions of GABA (40, 20, 8, 4, 2, 1, and 0.5 μg/mL) were used. The calibration curve of each compound was determined by plotting the ratio of peak area to internal standard versus concentration of the spiked standard solution. A linear regression equation (y = ax + b) was evaluated, where x is the concentration of the analytes and y is the peak area ratio. The correlation coefficient (R²) was calculated.

Zhang W., Yu W., Wang D., Wei L., Lee M, & Wang S. (2014). Effect of “Jian-Pi-Zhi-Dong Decoction” on Gamma-Aminobutyric Acid in a Mouse Model of Tourette Syndrome. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 407509.

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LC-MS Analysis of Phytochemicals

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LC-MS was conducted according to Lin method [17 (link)]. For this purpose, an Agilent series 6100 LC/MS system (Agilent Technologies, Santa Clara, CA, USA) with photodiode array detector was set at 260 nm. The UV spectra were examined with a range of 200 nm to 500 nm to obtain the maximum absorbance wavelength. A 150 mm × 3.0 mm, 3.5 µm Waters XTerra MS with a sentry guard column (Symmetry C18, 5 μm, 20 × 3.9 mm) was used. The mobile phase contained (1) water with 0.25% (v/v) acetic acid and (2) acetonitrile with 0.25% (v/v) acetic acid using linear gradient of 17–42% (v/v) (2) for 38 minutes. The temperature and flow rate were set to 45°C and 0.2 ml/min, respectively.
The LC system was joined to the mass spectrometer directly without stream splitting and, by using an electrospray interface Model HP 59987A, the ESI-MS spectra were obtained from the positive ion mode. The nebulizer pressure (N2) was 5.5 × 105 Pa and temperature of dying gas (N2) was set to 350°C, with a gas flow rate of 40 ml/min [17 (link)].

Akbari F., Azadbakht M., Bagheri A, & Vahedi L. (2022). In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae). Advances in Pharmacological and Pharmaceutical Sciences, 2022, 7865015.

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Quantitative LC-MS Analysis of Enzymatic Reactions

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All enzymatic reactions were performed in 20μL volume. After fixed reaction time, 20μL acetonitrile was added to terminate the reaction and precipitate proteins. Following centrifugation at 20,000g for 30min at 4°C, the supernatant was collected and dried using an Eppendorf Vacufuge concentrator (Hauppauge, NY). The sample was dissolved in aqueous solution and subjected to LC-MS analysis using an API3000 Triple Quadrupole mass spectrometer (AB Sciex, San Diego). Chromatography was performed at 0.2mL/min using a C18 column (XTerra MS, 5μm, 2.1mm×250 mm, Waters, Milford, MA). For MS separation, gradient started with 100% buffer A (water+0.1% formic acid (FA)) and linearly reached 20% buffer B (acetonitrile+0.1%FA) at 10 min. and 100% B at 25min. Product detection was performed using Xtracted Ion Chromatogram (XIC) based on theoretical m/z values.

Gupta R., Matta K.L, & Neelamegham S. (2015). A systematic analysis of acceptor specificity and reaction kinetics of five human α(2,3)sialyltransferases: Product inhibition studies illustrates reaction mechanism for ST3Gal-I. Biochemical and biophysical research communications, 469(3), 606-612.

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Pharmacokinetics of AM4113 in Mice

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We next conducted pharmacokinetic study of AM4113 to further understand it’s bioavailability. A group of mice were treated intraperitonially with a dose of 1 mg/kg of AM4113 and they were decapitated at various time points (10, 20, 40, 60, 90, 180, 300 and 420 min) under deep anesthesia (isoflurane). Brain and trunk blood were collected for the analysis of AM4113 using liquid chromatography-mass spectrometry (LC-MS) in the positive APCI mode. Following the addition of an internal standard (rimonabant) to the plasma (0.2 ml) or brain homogenate (0.5 ml), AM4113 and the internal standard were extracted by the addition of 0.5 ml of carbonate buffer (pH 9.6) and 3.0 ml of 3% isopropanol in hexane. After a gentle mix for 10 min, the samples were centrifuged at 2000 rpm. The organic phase was taken and evaporated to dryness in a vacuum centrifuge. The residue was then reconstituted with 150 μl of methanol:water (1:1). The extract was injected onto a C-18 column (XTerra MS, 3.0 × 100 mm, 3.5μ, Waters Corp., MA), eluted with an isocratic mobile phase (0.1% formic acid:methanol:acetonitrile [30:35:35]) at a flow rate of 0.5 ml/min, affording a retention time of 5.1 and 9.7 min for AM4113 and rimonabant, respectively. Both AM4113 and rimonabant were detected at their molecular ions. Each analysis was preceded with an eight-point linear calibration curve from 1000 to 7.5 ng/ml.

Balla A., Dong B., Shilpa B.M., Vemuri K., Makriyannis A., Pandey S.C., Sershen H., Suckow R.F, & Vinod K.Y. (2017). Cannabinoid-1 receptor neutral antagonist reduces binge-like alcohol consumption and alcohol-induced accumbal dopaminergic signaling. Neuropharmacology, 131, 200-208.

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