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Unity inova

Manufactured by Agilent Technologies
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The Unity Inova is a high-performance nuclear magnetic resonance (NMR) spectrometer developed by Agilent Technologies. It is designed for advanced analytical applications in various fields, including chemistry, materials science, and life sciences. The Unity Inova provides highly accurate and reproducible NMR data, enabling researchers and scientists to conduct in-depth analyses and characterizations of complex samples.

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Lab products found in correlation

Silica gel 60 f254, by Merck Group (2 mentions) Whatman no 1 sheets, by Cytiva (1 mentions) Diaion hp 20, by Merck Group (1 mentions) Merck silica gel 60 f254, by Merck Group (1 mentions) Rp c18, by Merck Group (1 mentions) Rp c18 f254 plates, by Merck Group (1 mentions) Silica gel, by Merck Group (1 mentions) Ft ir 4100 spectrometer, by Jasco (1 mentions) Triart c18 column, by YMC (1 mentions) 1260 infinity 2, by Agilent Technologies (1 mentions) Model 241 polarimeter, by PerkinElmer (1 mentions) Sio2 60, by Merck Group (1 mentions) Silica gel 60 pf254, by Merck Group (1 mentions) Sephadex lh 20, by Merck Group (1 mentions) Silica gel 60, by Merck Group (1 mentions) Microtof q 2, by Bruker (1 mentions) Expression cms mass spectrometer, by Advion (1 mentions) Fastdigest, by Thermo Fisher Scientific (1 mentions) Vnmrs, by Agilent Technologies (1 mentions) 700 mhz spectrometer, by Agilent Technologies (1 mentions)

51 protocols using unity inova

1

NMR Analysis of Organic Compounds

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Unless otherwise stated, all chemicals and reagents where from Merck-Millipore/Sigma-Aldrich. All NMR analyses were performed using 300 MHz Varian VNMRS, 400 MHz Varian Unity Inova or 600 MHz Varian Unity Inova instruments at the Central Analytical Facility (CAF) of the University of Stellenbosch. Chemical shifts (δ) are given in ppm, with the abbreviations indicating multiplicity (s = singlet, t = triplet, q = quartet, m = multiplet), coupling constant (J) in Hz, and integration of the observed signals. All restriction enzymes were purchased from New England Biolabs or Thermo Scientific (FastDigest).
Mostert K.J., Sharma N., van der Zwaag M., Staats R., Koekemoer L., Anand R., Sibon O.C, & Strauss E. (2021). The Coenzyme A Level Modulator Hopantenate (HoPan) Inhibits Phosphopantotenoylcysteine Synthetase Activity. ACS chemical biology, 16(11), 2401-2414.
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2

Spectroscopic Analysis of Organic Compounds

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The ultraviolet analysis (UV/VIS) was carried out employing a Shimadzu UV-visible recording spectrophotometer model-UV 240 (NRC, Cairo, Egypt). The nuclear magnetic resonance (1H- and 13C-NMR) analysis was performed via Varian Unity Inova (IET, Urbana, IL, USA). Mass spectroscopy was performed via Finnigan MAT SSQ 7000, 70 ev (Adaptas Solutions, Palmer, MA, USA). Flash column chromatography was employed using a Sephadex LH-20 (Sigma-Aldrich, Taufkirchen, Germany), obtained from Pharmacia Fine Chemicals, and silica gel (0.063–0.200 mm). Paper Chromatography (PC) was performed on Whatman No.1 sheets (Whatman Led. Maid Stone, Kent, UK) to quantify flavonoids and sugars in the extract. Thin-layer chromatography (TLC) analysis was performed on F254 plates. The solvent mixtures for separation were applied (n-butanol: acetic acid: water 4:1:5 upper phase, 15% acetic acid: water: glacial acetic acid: 85:15).
Selim S., Almuhayawi M.S., Alharbi M.T., Al Jaouni S.K., Alharthi A., Abdel-Wahab B.A., Ibrahim M.A., Alsuhaibani A.M., Warrad M, & Rashed K. (2022). Insights into the Antimicrobial, Antioxidant, Anti-SARS-CoV-2 and Cytotoxic Activities of Pistacia lentiscus Bark and Phytochemical Profile; In Silico and In Vitro Study. Antioxidants, 11(5), 930.
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3

PADA Degradation in Antibiotic Solutions

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The degradation of PADA was investigated using 1H NMR spectroscopy (300 MHz spectrometer, UNITY-INOVA, Varian). A 2-ml solution of 1% PADA and 5 wt% antibiotic (amoxicillin, cefazolin or vancomycin) was prepared. As a solvent, artificial saliva (1.3 g∙dm−3 NaHCO3, 1.2 g∙dm−3 KCl, 0.7 g∙dm−3 NaCl, 0.33 g∙dm−3 KSCN, 0.26 g∙dm−3 Na2HPO4 and 0.13 g∙dm−3 urea; pH = 7.4) was used. Prepared solutions were incubated at 37 °C with 60 rpm shaking. After 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, 12 h, 24 h and 48 h of degradation, the solutions were frozen and lyophilized, dissolved in the mixture of deuterated solvents CDCl3/DMSO (1:1 v/v) and analyzed via 1H NMR spectroscopy.
Leśniak-Ziółkowska K., Śmiga-Matuszowicz M., Blacha-Grzechnik A., Student S., Brzychczy-Włoch M., Krok-Borkowicz M., Pamuła E., Simka W, & Kazek-Kęsik A. (2020). Antibacterial and cytocompatible coatings based on poly(adipic anhydride) for a Ti alloy surface. Bioactive Materials, 5(3), 709-720.
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4

NMR Spectroscopy Analysis of Organic Compounds

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The NMR spectra were recorded in CDCl3 on a Varian Unity Inova at 500 MHz for 1H and 125 MHz for 13C (chemical shifts in δ, ppm). Column chromatography was performed using silica gel (230–400 mesh, SiliCycle Inc., Canada), Sephadex LH-20, and Diaion HP-20 (Sigma-Aldrich, USA). All solvents were analytical reagent grade and purchased from Labscan, Thailand. All reagents were purchased from Sigma, USA.
Chaniad P., Sudsai T., Septama A.W., Chukaew A, & Tewtrakul S. (2019). Evaluation of Anti-HIV-1 Integrase and Anti-Inflammatory Activities of Compounds from Betula alnoides Buch-Ham. Advances in Pharmacological Sciences, 2019, 2573965.
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5

Spectroscopic Analysis of Organic Compounds

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UV spectra were recorded using a Thermo spectrometer. IR spectra were recorded using a JASCO FT/IR-4100 spectrometer (JASCO, Easton, MD, USA). The 1D- and 2D-NMR spectra were obtained using Varian Unity Inova 400 MHz and 500 MHz spectrometers with tetramethylsilane (TMS) as an internal standard, and the chemical shifts were recorded as δ values (ppm). Mass spectra were recorded using a Thermo Scientific™ (Q Exactive) EASY-nLC 1000 spectrometer (San Jose, CA, USA). Silica gel (Merck, Darmstadt, Germany; 63–200 µm particle size), RP-C18 (Merck; 75 µm particle size), sephadex LH-20 (Pharmacia, Uppsala, Sweden ), and dianion HP-20 (Supelco, St. Louis, MI, USA) were used for column chromatography. TLC was performed using Merck Silica gel 60 F254 and RP-C18 F254 plates (Merck, Darmstadt, Germany). Preparative high-performance liquid chromatography (HPLC) was performed using a Water System with a UV detector 2996 (Waters, Miford, MA, USA) and a YMC-Triart C18 column (10 mm × 250 mm, 5 μm particle size; YMC Co., Ltd., Kyoto, Japan). Compounds were visualized after spraying with aqueous 10% H2SO4 and heating for 3–5 min.
Tran H.N., Yu J.S., Huang T., Lee G., Choi H.S, & Yang H.O. (2024). Neuroprotective Effects of Chemical Constituents of Leaves of Euonymus hamiltonianus Wall.. Plants, 13(8), 1094.
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6

Characterization of organic compounds by NMR

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NMR spectra were recorded on a 600 MHz Varian Unity Inova (151 MHz for 13C NMR spectra) at the Central Analytical Facilities (CAF) of Stellenbosch University. 1H and 13C spectra are referenced to the residual CDCl3 solvent signal (δH 7.26 or δC 77.16 ppm). NMR spectra were processed using MestReNova version 11.0.3-18688.
van der Westhuyzen A.E., Ingels A., Rosière R., Amighi K., Oberer L., Gustafson K.R., Wang D., Evidente A., Maddau L., Masi M., de Villiers A., Green I.R., Berger W., Kornienko A., Mathieu V, & van Otterlo W.A. (2020). Deciphering the chemical instability of sphaeropsidin A under physiological conditions – degradation studies and structural elucidation of the major metabolite. Organic & biomolecular chemistry, 18(40), 8147-8160.
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7

Longitudinal fMRI Study of Bipolar Disorder

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All subjects were scanned using a 4.0 Tesla Varian Unity INOVA MRI scanner. Prior to beginning the study, subjects could familiarize themselves with the scanner to decrease any anxiety. Subjects completed anatomical T1-weighted, 3-D brain scans first and then completed an fMRI session in which scans were acquired while performing the CPT-IP task using a T2*-weighted gradient-echo EPI pulse sequence. The image acquisition details were provided in the Supplement 1, available online.
All participants were scanned three times: at baseline, week one and week six. After excluding participants who did not complete all three scans and clinical evaluations, we excluded additional participants that had excessive head motion during MRI scans (two patients with BD receiving lithium treatment, two patients with BD receiving quetiapine treatment and one healthy individual), a total of 71 patients with BD (45 patients receiving quetiapine monotherapy and 26 patients receiving lithium carbonate monotherapy) and 55 healthy individuals were included in statistical analyses.
Li W., Lei D., Tallman M.J., Ai Y., Welge J.A., Blom T.J., Fleck D.E., Klein C.C., Patino L.R., Strawn J.R., Gong Q., Strakowski S.M., Sweeney J.A., Adler C.M, & DelBello M.P. (2022). Pretreatment Alterations and Acute Medication Treatment Effects on Brain Task-related Functional Connectivity in Youth With Bipolar Disorder: A Neuroimaging Randomized Clinical Trial. Journal of the American Academy of Child and Adolescent Psychiatry, 61(8), 1023-1033.
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8

Synthesis and Characterization of Novel Compounds

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All reagents were purchased from Oakwood Chemicals and used as supplied. 1H NMR spectra and 13C NMR spectra were collected on the Varian Unity INOVA (500 MHz) in DMSO-d6. Chemical shifts are reported in ppm relative to the reference signal and coupling constant (J) values are reported in hertz (Hz). LC/MS was recorded using an Agilent InfinityLab MSD single quadrupole mass spectrometer equipped with an API-ES and an Agilent Infinity II 1260 HPLC equipped with an Agilent Infinity 1260 variable wavelength detector and a Phenomenex Synergi 2.5 mm Hydro-RP 100A C18 30×2 mm column. HPLC Method used: starting with a flow rate of 0.6 mL/min for 0.4 minutes at 20% solvent B followed by a 1.6-minute gradient of 20–95% solvent B at 0.6 mL/min followed by 2 minutes at 95% solvent B with a gradual ramp up of the flow rate to 1.2 mL/min at the end (solvent A, water with 0.1% formic acid; solvent B, acetonitrile with 0.1% formic acid and 5% water; absorbance monitored at 220 and 280 nm). MS Method used: using atmospheric pressure ionization-electrospray, positive and negative ions were monitored in the range of 70–700. Melting points were determined on a MEL-TEMP®.
Decker A.M., Brackeen M.F., Mohammadkhani A., Kormos C.M., Hesk D., Borgland S.L, & Blough B.E. (2022). Identification of a Potent Human Trace Amine-Associated Receptor 1 Antagonist. ACS chemical neuroscience, 13(7), 1082-1095.
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9

NMR Characterization of Peroxiredoxin Proteins

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1H‐15N HSQC spectra were recorded at 20°C on a Varian Unity Inova 600 MHz spectrometer equipped with room‐temperature Varian 5 mm Triple‐resonance H/C/N inverse‐detection solution probe with Z gradient probe. Samples were prepared for in their various buffers and included 10% D2O. Prx was used at 400 μM concentration in the following buffers all at pH 7.0, 20 mM sodium phosphate, 20 mM sodium phosphate containing 6.66 M urea, 20 mM sodium phosphate containing Na2SO4 (at 250 mM, 500 mM and 700 mM). For the Prx and ComR DBD complex, 500 μM of unlabeled DBD was added to 15N labeled Prx in 20 mM sodium phosphate pH 7.0. 15N Prx‐6His samples were measured at 400 μM in pH 7.0 20 mM sodium phosphate buffer. Data was processed using NMRpipe (Delaglio et al., 1995 (link)) and analyzed using UCSF Sparky.
Asakereh I., Rutbeek N.R., Singh M., Davidson D., Prehna G, & Khajehpour M. (2024). The Streptococcus phage protein paratox is an intrinsically disordered protein. Protein Science : A Publication of the Protein Society, 33(6), e5037.
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10

NMR Spectroscopy of Monomers

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1H NMR and 13C NMR spectra of the monomers were recorded in the CDCl3 solution, using TMS as an internal standard. A 300 MHz NMR spectrometer (UNITY/INOVA, Varian, Palo Alto, CA, USA) was employed for these experiments.
Barszczewska-Rybarek I.M., Chrószcz M.W, & Chladek G. (2020). Novel Urethane-Dimethacrylate Monomers and Compositions for Use as Matrices in Dental Restorative Materials. International Journal of Molecular Sciences, 21(7), 2644.
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