An Anton Paar Litesizer 2000 was used to measure both the size distribution via dynamic light scattering (DLS) and zeta potential. For DLS, two 500 μL aliquots of 1 mg/mL particle solutions in PBS, unless otherwise specified, from two separate batches were mixed to provide a representative scattering curve. The intensity weighted scattering curves were reported. Transmission electron microscopy (TEM) images of the nanoparticles were taken using a T12 Spirit (FEI Tecnai) microscope. TEM samples were prepped by dispersing the lipid-coated hydrophobically modified particles in 1% uranyl acetate aqueous solution and then drying them onto a carbon TEM grid. TEM images of the P@hMSNs can be found in the Supplemental Information as Figure S1 with a hard sphere diameter of about ~110 nm.
T12 spirit
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The T12 Spirit is a compact and versatile laboratory centrifuge from Thermo Fisher Scientific. It is designed to efficiently separate samples in various laboratory applications. The T12 Spirit features a fixed-angle rotor with a capacity of up to 12 tubes, accommodating sample volumes from 1.5 to 15 mL. The centrifuge operates at a maximum speed of 12,100 rpm, providing a relative centrifugal force (RCF) of up to 15,344 x g. The T12 Spirit is a reliable and functional laboratory equipment piece.
Automatically generated - may contain errors
Lab products found in correlation
10 protocols using t12 spirit
1
Characterization of Lipid-Coated Hydrophobic Nanoparticles
2
Hydrophobic Nanoparticle TEM Imaging
3
Synthesis and Characterization of Carbonized Silica Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 3
6 gr aqueous silica dispersion (Ludox HS 30 from Aldrich) was diluted with 80 g of distilled water and was placed under stirring at 70° C. 3 g of (3-Aminopropyl)triethoxysilane was added dropwise and the mixture was allowed to react for 24 h at 70° C. under reflux. The product was extensively dialyzed against deionized water using a Snake Skin Pleated Dialysis Tubing membrane (with a molecular weight cutoff of 3500 Da). The purified dispersion was freeze-dried. TGA analysis suggested that the organic content of the particles was 7 wt %. The powder was then thermally treated at 300° C. for 1 h to allow the carbonization of the organic groups.
Transmission Electron Microscopy (TEM) images were obtained by A FEI T12 Spirit operated at 120 kV. A droplet of a sample (0.05 mg/mL in water) was deposited on a carbon coated cupper grid (Agar Scientific, USA) and dried under air.
4
Automated TEM Acquisition for 2D Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Micrographs of untilted and −55˚ tilted views were acquired on a FEI T12 Spirit operated at 120 kV, spot size 5, 52000x nominal magnification, pixel size 2.07 Å, defocus values between −0.7 and −1.5 μm, and a dose of 30 e-/Å2. Data collection was automated using the MSI-RCT application within the Leginon software package (Suloway et al., 2005 (link)). For 2-D analysis of labeled complexes, data was collected using the MSI-Raster application in Leginon.
5
Visualizing N558 Interactions with Tubulin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N558 was viewed on its own or in complex with tubulin oligomers by electron microscopy. For negative staining, N558 was allowed to adsorb to copper grids coated with a thin film of carbon, rinsed with buffer, then with 1% uranyl acetate, blotted, dried, and imaged with a T12 Spirit (FEI). For shadow imaging, N558 was diluted into 100 mM ammonium acetate buffer, pH 7.0, with 50% glycerol, applied to freshly cleaved mica, rapidly frozen, freeze-dried in a Bal Tec BAP 060 freeze-etch instrument and rotary shadowed with a thin layer of platinum-carbon, and imaged on the same microscope. For studies of N558 with curving tubulin, tubulin was induced to form rings, rather than curls, by mixing phosphocellulose-purified protein at 20 µM with 40 µM dolastatin-10, following the procedure of Moores and Milligan (2008) (link). Rings were mixed with recombinant proteins at a molarity equal to that of tubulin, incubated at room temperature for 10 min, and applied to a freshly glow-discharged carbon-coated Formvar grid. Negatively stained samples were prepared as described at the beginning of this paragraph and examined in an electron microscope (CM10; Philips) operating at 80 KeV. Shadowed samples were prepared and imaged at the electron microscopy laboratory in ETH Zurich. Images were recorded on charge coupled device cameras and studied without further image processing.
6
Comprehensive Nanoparticle Characterization Protocol
7
Tau Fibrils Electron Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tau fibrils or tau fibrils-chaperone mixtures (10 µl) were deposited on glow discharged carbon-coated copper EM grids (Electron Microscopy Sciences), washed with three consecutive drops of 1% w/v Uranylformate, and air-dried. Imaging was performed on an FEI T12 Spirit transmission electron microscope at 120kV and a magnification of 9300-30000 times, equipped with a Gatan OneView CMOS 4K x 4K CCD camera.
8
Visualization of Taxol-Stabilized Microtubules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sample preparation and imaging were performed essentially as described previously12 (link). In brief, taxol-stabilized microtubules (36 nM polymerized tubulin) were incubated with 20 nM Dam1WT complex or Dam1OD complex. A 2-μl drop of sample was applied to a negatively charged carbon-coated copper grid (EMS). Grids were washed with BRB80 (80 mM PIPES, 120 mM K+, 1 mM MgCl2, and 1 mM EGTA, pH 6.9) and then stained with 0.075% uranyl formate. Images were collected on a Spirit T12 (FEI) at 52,000x magnification at the specimen level with 1 sec exposure.
9
Visualization of Taxol-Stabilized Microtubules
10
Isolation and Characterization of Salivary Exosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell-free saliva samples (10 ml) were collected from the authors. The specimens were centrifuged at 2,000 g for 30 min, and the resulting supernatant was centrifuged for 45 min at 12,000 g at 4°C. The obtained supernatant was filtered through a 0.45 μm filter membrane, followed by centrifuging at 110,000 g for 70 min. The supernatant was discarded, and the pellets were resuspended in PBS, and re-centrifuged at 110,000 g for 70 min. Finally, the particles were resuspended with cold PBS for further analysis. The morphology of particles was evaluated using transmission electron microscopy (TEM) (Tecnai Spirit T12, FEI) and the sizes were analyzed by NanoSight (Flow NanoAnalyzer, NanoFCM, Xiamen, China). Briefly, 10 μl saliva-Exos was loaded on carbon-coated copper grids for 1 min, then 10 μl phosphotungstic acid was added to the copper grids for another 1 min. Grids were viewed using TEM and photographed for further analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!