Mononuclear cells isolated from patient bone marrow samples were used to prepare cytospins with glass slides treated by Poly-L-lysine (PLL) and then fixed in cold acetone. After fixation, mononuclear cells were briefly washed with 0.5% Triton X-100 in phosphate buffered saline (PBS) for 10 min; non-specific binding was then blocked by incubation for 1 h in 5% goat serumsealing fluid. The samples were incubated with the primary antibody (anti-RBP2 antibody,1:100, Abcam) overnight at 4°C. The mononuclear cells were then washed three times for 5 min in PBS, followed by incubation with their respective Alexa Fluor-conjugated secondary antibodies (goat anti-rabbit IgG, 1:1000,CST) for 1 h in the dark. After four washes of 5 min each, mononuclear cells were stained with 4', 6-diamidino-2-phenylindole (DAPI) for 5 min. Slides were examined by confocal laser scanning microscopy.
Anti rbp2 antibody
Manufactured by Abcam
Anti-RBP2 antibody is a laboratory reagent used in research applications. It is a specific antibody that binds to and detects the RBP2 protein, which is involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of RBP2 in biological samples.
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4 protocols using anti rbp2 antibody
1
Immunofluorescence Analysis of RBP2 in Bone Marrow
2
Immunohistochemical Analysis of RBP2
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Mononuclear cells isolated from patient bone-marrow samples were used to prepare cytospins with glass slides treated by Poly-L-lysine (PLL), then fixed in ice-cold acetone. Samples were stained with anti-RBP2 antibody (1:150, Abcam) overnight at 4°C, then horseradish peroxidase-conjugated secondary antibody for 30 min.
3
Immunohistochemical Analysis of Key Cellular Markers
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Paraffin embedded slides were deparaffinized, rehydrated and subjected to antigen-retrieval using citric acid buffer. The endogenous peroxidase was deactivated by H2O2. The slides were blocked using a 10% goat serum solution and incubated with the corresponding primary antibodies overnight at 4°C. The used antibodies were: anti-RBP2 antibody (1:150, Abcam), anti-p65 antibody (1:150, Abcam) and anti-Ki67 antibody (1:100, Abcam). Next, the slides were incubated with a secondary antibody, followed by a colorimetric detection using a DAB staining kit (Vector Laboratories, USA).
4
Immunocytochemical analysis of bone marrow cells
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The mononuclear cells, that were isolated from patient bone-marrow samples, were used to prepare cytospins with glass slides xed by a polyformaldehyde xation solution. The samples were stained with anti-RBP2 antibody (1:150, Abcam) and anti-p65 antibody (1:150, Abcam) overnight at 4°C, followed by an incubation with a horseradish peroxidase-conjugated secondary antibody for 30 min.
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