Total stat3 c 20 sc 482
Total STAT3 (C-20: sc-482) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that recognizes the STAT3 protein, which is a transcription factor involved in various cellular processes. The product is intended for use in research applications that require the detection or measurement of STAT3 levels, such as Western blotting, immunoprecipitation, and immunohistochemistry.
Lab products found in correlation
2 protocols using total stat3 c 20 sc 482
Comprehensive Protein Analysis by Western Blot
Analysis of Keratinocyte Differentiation in HPV18 Infection
For analysis of Phospho-STAT3 (S727) (ab32143, abcam), Total STAT3 (C-20: sc-482, Santa Cruz Biotechnology and 9132, CST), involucrin (SY5, Santa Cruz Biotechnology) and HPV18 E1^E4 (mouse monoclonal antibody 1D11 [84 ]) expression, the formaldehyde-fixed raft sections were treated with the sodium citrate method of antigen retrieval. Briefly, sections were boiled in 10 mM sodium citrate with 0.05% Tween-20 for 10 minutes. Sections were incubated with appropriate antibodies and immune complexes visualized by using Alexa 488 and 594 secondary antibodies (Invitrogen). The nuclei were counterstained with the DNA stain 4’,6-diamidino-2-phenylindole (DAPI) and mounted in Prolong Gold (Invitrogen).
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