Phosphoglucose isomerase from rabbit muscle

Phosphoglucose isomerase from rabbit muscle (commercially available from Sigma Aldrich code P9544-1KU), phosphomannose isomerase from E. coli, glucose-6-phosphate dehydrogenase from bakers’ yeast (S. cerevisiae), phosphoglucomutase from rabbit muscle (commercially available from Sigma Aldrich code P3397-1KU), α-D-glucose 1,6-bisphosphate potassium salt hydrate, α-D-glucose 1-phosphate disodium salt hydrate, and β-nicotinamide adenine dinucleotide phosphate sodium salt were purchased from Sigma-Aldrich. α-D-mannose 1,6-bisphosphate was prepared and purified as described [18 (link)]. SYPRO Orange was from Invitrogen Molecular Probes. All other reagents were of analytical grade.

DEAE-Sepharose ff, Superdex-75, Butyl Sepharose ff, were purchased from GE Healthcare Life Sciences. Phosphoglucose isomerase from rabbit muscle, phosphomannose isomerase from E. coli, glucose 6-phosphate dehydrogenase from baker's yeast (S. cerevisiae), phosphoglucomutase from rabbit muscle, α-D-Glucose 1,6-bisphosphate potassium salt hydrate, α-D-Glucose 1-phosphate disodium salt hydrate, α-D(+)Mannose 1-phosphate sodium salt hydrate, D-Glucose 6-phosphate, D-Mannose 6-phosphate, β-Nicotinamide adenine dinucleotide phosphate sodium salt, were purchased from Sigma-Aldrich. Sypro Orange was from Invitrogen Molecular Probes. Sodium orthovanadate was from Acros Organics. Creatine phosphate disodium salt tetrahydrate was from Alfa Aesar. Clodronate ((Dichloro-phosphono-methyl)phosphonic acid) and neridronate ((6-Amino-1-hydroxyhexane-1,1-diyl)bis(phosphonic acid) were from ABIOGEN-PHARMA.
Mannose 1,6-bisphosphate was synthesized as described [18 (link)] and purified on an AG1x8 hydroxide form column by running a step gradient 0–1 M NaCl. Fractions were analyzed by ¹H- and ³¹P-NMR spectroscopy. An internal standard (Trimethylsilylpropanoic acid) was used in order to measure the concentration. All the other reagents were of analytical grade. The ORF encoding QDK-PMM1 was obtained by de novo gene synthesis and was purchased by GeneCust, Luxemburg; it is available upon request.

DEAE-Sepharose Fast Flow, Superdex 75, and thrombin were purchased from GE Healthcare. Phosphoglucose isomerase from rabbit muscle, phosphomannose isomerase from Escherichia coli, glucose-6-phosphate dehydrogenase from bakers' yeast (Saccharomyces cerevisiae), α-d-glucose 1,6-bisphosphate potassium salt hydrate, α-d-glucose 1-phosphate disodium salt hydrate, and β-nicotinamide adenine dinucleotide phosphate sodium salt were purchased from Sigma-Aldrich. SYPRO Orange was from Invitrogen Molecular Probes. Sodium orthovanadate was from Acros Organics. All other reagents were of analytical grade.