Py416 src

Manufactured by Thermo Fisher Scientific

The PY416 Src is a laboratory instrument used for the detection and analysis of the Src protein, a key regulator of cellular signaling pathways. The core function of this product is to provide researchers with a reliable and accurate tool for studying the activity and expression of the Src protein, which is involved in various cellular processes, including cell growth, differentiation, and survival.

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Lab products found in correlation

Alexa 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Py397 fak, by Thermo Fisher Scientific (1 mentions) Gapdh, by Merck Group (1 mentions) Diphenyliodonium dpi, by Merck Group (1 mentions) 4 iodophenylboronic acid 4ipba, by Merck Group (1 mentions) Catalase cat, by MP Biomedicals (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) Oct compound, by Sakura Finetek (1 mentions) Fluorescence microscope, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Alexa fluor 594 conjugated griffonia simplicifolia isolectin b4, by Thermo Fisher Scientific (1 mentions)

2 protocols using py416 src

Oxidative Stress Response in HT-29 Cells

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HT-29 human colon carcinoma was obtained from ATCC and maintained in DMEM containing 10% FBS (Omega Scientific), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, and 100 units/ml penicillin and 100 μg/ml streptomycin. Diphenyliodonium (DPI), N-acetyl-L-cysteine (NAC), phorbol 12-myristate 13-acetate (PMA), horseradish peroxidase (HRP), luminol, H₂O₂, and 4-iodophenylboronic acid (4IPBA) were purchased from Sigma-Aldrich. Superoxide dismutase (SOD) was purchased from Abnova, and catalase (CAT) was purchased from MP Biomedicals.
FAK (Millipore), pY397 FAK (Invitrogen), pY402 Pyk2 (Cell Signaling), pY416 Src (Invitrogen), GAPDH (Millipore) antibody, and Alexa 488 phalloidin (Invitrogen) were obtained.

Kim J.S., Jeong K., Murphy J.M., Rodriguez Y.A, & Lim S.T. (2019). A Quantitative Method to Measure Low Levels of ROS in Nonphagocytic Cells by Using a Chemiluminescent Imaging System. Oxidative Medicine and Cellular Longevity, 2019, 1754593.

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Immunofluorescence Analysis of Eye Tissue

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The eyes were enucleated, fixed, embedded in optimum cutting temperature (OCT) compound (Sakura Finetek, Torrance, CA) or paraffin, sectioned, and mounted on slides. First, the endogenous peroxidase activity in the tissues was quenched. The sections were blocked with 10% normal goat serum and were then incubated with primary immunoglobulin G (IgG) against phospho-Y416 Src (p-Y416 Src; Invitrogen) or glial fibrillary acidic protein (GFAP; Dako), followed by the appropriate fluorescent secondary IgG (Dako). Blood vasculature was visualized by costaining with Alexa Fluor^® 594-conjugated Griffonia simplicifolia isolectin B4 (Invitrogen). Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Burlingame, CA). The images were acquired using a fluorescence microscope (Olympus), and six to eight sections were examined per group.

Seo S, & Suh W. (2017). Antiangiogenic effect of dasatinib in murine models of oxygen-induced retinopathy and laser-induced choroidal neovascularization. Molecular Vision, 23, 823-831.

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