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Anti il 21

Manufactured by Thermo Fisher Scientific

Anti-IL-21 is a laboratory reagent that binds to and neutralizes the cytokine interleukin-21 (IL-21). It can be used in various in vitro and ex vivo research applications.

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2 protocols using anti il 21

1

Cytokine-Driven T Cell Stimulation

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MultiScreen HTS plates were coated overnight at 4°C with anti-IL-21 and/or anti-IL-4 (eBioscience). Sorted cells were cultured (0.25 × 105 cells/well) with PMA (50ng/mL) and ionomycin (1μg/mL) for 36 h at 37°C followed by adding primary then secondary detection antibodies (Supplementary Table 2). Spots were developed with Vector Blue (Vector Laboratories) and AEC (BD Biosciences). Spots were quantified using an ImmunoSpot analyzer (Cellular Technology Limited).
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2

Cytokine-Driven T Cell Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MultiScreen HTS plates were coated overnight at 4°C with anti-IL-21 and/or anti-IL-4 (eBioscience). Sorted cells were cultured (0.25 × 105 cells/well) with PMA (50ng/mL) and ionomycin (1μg/mL) for 36 h at 37°C followed by adding primary then secondary detection antibodies (Supplementary Table 2). Spots were developed with Vector Blue (Vector Laboratories) and AEC (BD Biosciences). Spots were quantified using an ImmunoSpot analyzer (Cellular Technology Limited).
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