Cells were washed with PBS and lysed in lysis buffer (0.1% Triton X-100, 150 mM KCl, 50 mM HEPES, pH 7.4 and 1 mM DTT plus proteinase inhibitor mixture (Roche), including 1 mM phenylmethylsulfonyl fluoride (PMSF)). Equal amounts of proteins were separated on 12% SDS-PAGE and blotted onto nitrocellulose (Schleicher & Schuell). Western blots were probed with the following antibodies: rabbit polyclonal anti-RALY (Bethyl); mouse monoclonal anti-hnRNP A1 (Novus Biologicals); mouse monoclonal anti-Annexin A1 (Santa Cruz Biotechnology); rabbit polyclonal anti-Histone H1X (Abcam); rabbit polyclonal anti-PABP (Abcam); rabbit polyclonal anti-RPL26 (Abcam); mouse monoclonal anti-Tubulin (Santa Cruz Biotechnology); mouse monoclonal anti-Actinin (Santa Cruz Biotechnology). Horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies (Santa Cruz Biotechnology) were used as secondary antibodies.
Horseradish peroxidase hrp conjugated goat anti mouse and anti rabbit antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies are laboratory reagents used for immunodetection. They consist of goat-derived antibodies that specifically recognize and bind to mouse or rabbit antibodies, conjugated to the enzyme horseradish peroxidase. These conjugated antibodies can be used to detect and visualize the presence of target proteins in various immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti tubulin, by Santa Cruz Biotechnology (1 mentions)
Mouse monoclonal anti actinin, by Santa Cruz Biotechnology (1 mentions)
Proteinase inhibitor mixture, by Roche (1 mentions)
Mevalonolactone, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Anti egfr, by Santa Cruz Biotechnology (1 mentions)
Anti hmgcr, by Santa Cruz Biotechnology (1 mentions)
Anti phospho src, by Cell Signaling Technology (1 mentions)
Water soluble cholesterol, by Merck Group (1 mentions)
Anti src, by Santa Cruz Biotechnology (1 mentions)
Anti fatty acid synthase fasn, by Cell Signaling Technology (1 mentions)
Methyl β cyclodextrin mβcd, by Merck Group (1 mentions)
2 protocols using horseradish peroxidase hrp conjugated goat anti mouse and anti rabbit antibodies
1
Western Blotting for Protein Expression
2
Characterizing Lipid Raft Dynamics in Cells
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Alexa Fluor 488-conjugated cholera toxin subunit B (CTxB) was obtained from Molecular Probes (Eugene, OR, USA).
The anti-EGFR, anti-Src, anti-HMG-CR, anti-sterolregulatory element-binding protein (SREBP)-1, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-Src and anti-fatty acid synthase (FASN) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Antiphospho-EGFR (1068) was obtained from Invitrogen/Life technologies (Carlsbad, CA, USA). Doxorubicin, methyl-βcyclodextrin (MβCD), water-soluble cholesterol, and simvastatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mevalonolactone, which turns to mevalonate [20] , was purchased from Sigma-Aldrich.
The anti-EGFR, anti-Src, anti-HMG-CR, anti-sterolregulatory element-binding protein (SREBP)-1, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-Src and anti-fatty acid synthase (FASN) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Antiphospho-EGFR (1068) was obtained from Invitrogen/Life technologies (Carlsbad, CA, USA). Doxorubicin, methyl-βcyclodextrin (MβCD), water-soluble cholesterol, and simvastatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mevalonolactone, which turns to mevalonate [20] , was purchased from Sigma-Aldrich.
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