Oxidized manganese, Mn (II) immediately produced in the aqueous phase, treated as a key factor investigating the oxidation of elemental manganese was quantified spectrophotometrically using the formaldoxime methods32 (link) (Mn (II) or even Mn (III) would be oxidized to Mn (IV) under alkaline condition) with a UV spectrophotometer (V-560 UV/VIS Spectrophotometer, Jasco, Japan). The concentrations of volatile fatty acids (VFAs) were determined using a high performance liquid chromatography (Shimadzu, LC-20AT, Japan), equipped with an elite Hypersil ODS2 C18 column (25 μm, 4.6 × 250 mm) for separation at 40 °C and a diode array detector (SPD-M20A, Japan) for measurement at 210 nm. The mobile phase consisted of methanol (15%, V/V) and ultrapure water (pH 3.0, 85%, V/V) at a flow rate of 1 mL/min.
V 560 uv vis spectrophotometer
Manufactured by Jasco
Sourced in United States, Japan
The V-560 UV/Vis spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light through a sample across a range of ultraviolet and visible wavelengths. It is capable of performing accurate spectral analysis and quantitative measurements of various types of samples.
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Lab products found in correlation
Eclipse fluorescence spectrophotometer, by Agilent Technologies (2 mentions)
Lc 20at, by Shimadzu (1 mentions)
Jms 700 mstation, by JEOL (1 mentions)
Unity plus 500, by Agilent Technologies (1 mentions)
Silica gel 60, by Kanto Chemical (1 mentions)
Pu 2086 pump, by Jasco (1 mentions)
Pack diol 120 np, by YMC (1 mentions)
Uv 970 detector, by Jasco (1 mentions)
Ri 2031 detector, by Jasco (1 mentions)
Cosmosil 5sl 2, by Nacalai Tesque (1 mentions)
Unity inova 500 mhz spectrometer, by Agilent Technologies (1 mentions)
Inverse detection probe id pfg, by Agilent Technologies (1 mentions)
Fp 8300 spectrofluorometer, by Jasco (1 mentions)
X pert mpd diffractometer, by Philips (1 mentions)
Isv 469, by Jasco (1 mentions)
Complete protease inhibitor cocktail, by Merck Group (1 mentions)
Tm4000plus, by Hitachi (1 mentions)
241 polarimeter, by PerkinElmer (1 mentions)
Avance 600 mhz instrument, by Bruker (1 mentions)
5 mm tci inverse detection cryoprobe, by Bruker (1 mentions)
21 protocols using v 560 uv vis spectrophotometer
1
Quantifying Manganese Oxidation by Spectrophotometry
2
Spectroscopic Analysis of Organic Compounds
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A JASCO P1020 NK digital polarimeter was used to measure the optical rotations. The diffuse reflectance method was used to record IR spectra on a JASCO FT/IR-410 spectrophotometer. UV spectra were obtained on a JASCO V-560 UV/Vis spectrophotometer. ECD spectra were measured on a JASCO J-725N spectrophotometer. 1H and 13C NMR spectra were recorded on a JEOL JNM-AL 400 (1H: 400 MHz) or Varian Unity plus 500 (1H: 500 MHz, 13C: 126 MHz, respectively) spectrometer using CDCl3. Chemical shift values are given in δ (ppm), using the solvent peak signals (CDCl3: TMS) as references, and coupling constants (J) are reported in Hz. A JEOL JMS-700 MStation was used to record mass spectra, including high-resolution spectra. Column chromatography was performed on Silica gel 60 (100–210 mesh, Kanto Chemical Co., Inc., Tokyo, Japan). Preparative HPLC was performed on a JASCO chromatograph (n-hexane–EtOAc, CHCl3–EtOAc) equipped with a JASCO PU-2086 pump, a JASCO UV-970 detector, a JASCO RI-2031 detector, and various columns: COSMOSIL 5SL-II (10 × 250 mm, Nacalai Tesque Inc., Kyoto, Japan), COSMOSIL 5SL-II (4.6 × 250 mm, Nacalai Tesque Inc., Kyoto, Japan), YMC-Pack Diol-120-NP (4.6 × 250 mm, YMC Co., Ltd., Kyoto, Japan), and Inertsil CN-3 (4.6 × 250 mm, GL Sciences, Tokyo, Japan).
3
Absorption and Fluorescence Spectroscopy of Peptide-Graphene Oxide Interactions
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Absorption spectra for each system were recorded in the 200–700 nm λ range using a JASCO V-560 UV-vis spectrophotometer equipped with a 1 cm path-length cell.
Fluorescence spectra were recorded with a Cary Eclipse Fluorescence spectrophotometer, which had 0.5 nm resolution at room temperature. The spectra were collected using 5:2.5 nm slit widths for all measurements. The excitation wavelengths of 488 nm and 543 nm were used to excite the fluorescence of carboxyfluorescein (FAM) and rhodamine (Rhod), respectively. In the FRET experiments, the fluorescence spectra of the three pep−GO systems were compared with those of the free peptides in the first experiment and with the spectra obtained from the interaction between the SUVs and the pep−GO hybrids in the second experiment, which quantified the energy transfer process that happened in the two cases.
Fluorescence spectra were recorded with a Cary Eclipse Fluorescence spectrophotometer, which had 0.5 nm resolution at room temperature. The spectra were collected using 5:2.5 nm slit widths for all measurements. The excitation wavelengths of 488 nm and 543 nm were used to excite the fluorescence of carboxyfluorescein (FAM) and rhodamine (Rhod), respectively. In the FRET experiments, the fluorescence spectra of the three pep−GO systems were compared with those of the free peptides in the first experiment and with the spectra obtained from the interaction between the SUVs and the pep−GO hybrids in the second experiment, which quantified the energy transfer process that happened in the two cases.
4
Characterization of Organic Compounds
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The NMR experiments were carried out at 27°C on a Varian UNITY Inova 500 MHz spectrometer (1H at 499.88 MHz, 13C NMR at 125.7 MHz) equipped with pulse field gradient module (Z axis) and a tuneable 5 mm Varian inverse detection probe (ID-PFG). ESI mass spectra were acquired on a API 2000– ABSciex using CH3OH (positive ion mode). A JASCO V-560 UV-Vis spectrophotometer equipped with a 1 cm path-length cell was used for the UV-Vis measurements. Luminescence measurements were carried out using a Cary Eclipse Fluorescence spectrophotometer with resolution of 0.5 nm, at room temperature. The emission was recorded at 90° with respect to the exciting line beam using 10:10 slit-widths for all measurements. All chemicals were reagent grade and were used without further purification.
5
Optical Properties of Ojikoku Petals
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UV/Vis spectra as well as the color parameters of the O. tetraptera petals were measured by a JASCO V-560 UV/Vis spectrophotometer equipped with an integral sphere. The upper edges of the petals were cut into 1.5 × 1.5 cm squares for use in these analyses.
6
Comprehensive Materials Characterization
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Powder X-ray Diffraction (XRD) analysis was performed on a Philips X'PertMPD diffractometer (Cu-Ka = 0.15406 nm). The Brunauer-Emmett-Teller (BET) specific surface area (SBET) was determined from N2 adsorption-desorption isotherms at 196°C, in a Quantachrome Nova 4200e apparatus. Temperature programmed reduction (TPR) was carried out in an AMI-200 (Altamira Instruments) system. The H2 consumption was followed by a thermal conductivity detector (TCD) and by a mass spectrometer (Dymaxion 200 amu, Ametek). Transmission electron microscopy (TEM) micrographs were obtained using a LEO 906E microscope operating with an accelerating voltage of 120 kV. Diffuse reflectance (DR) UV-Vis spectra of the powder samples were recorded on a JASCO V-560 UV-Vis spectrophotometer, equipped with an integrating sphere attachment (JASCO ISV-469). The reflectance spectra were converted by the instrument software (JASCO) to equivalent absorption Kubelka–Munk units. Steady-state photoluminescence (PL) spectra were recorded at room temperature on a JASCO FP-8300 spectrofluorometer equipped with a 150 W Xe lamp. The morphology and elemental mapping of the materials was obtained by SEM/EDXS analysis using a FEI Quanta 400FEG ESEM/EDAX Genesis X4M instrument.
7
Absorption Spectra of Black Metallomesogen Dye
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Example 12
Absorption Spectra of Black Metallomesogen Dye
We have made a black dye mixture by mixing the three synthesized red, yellow and blue MOM components of examples 7, 8 and 9, respectively. Equal weight of three dyes were mixed and dissolved in CHCl3 solvent. The absorption spectra of the black MOM dye were measured by JASCO V-560 UV-VIS spectrophotometer. The measurements of the spectra were carried out on a 0.1 micron thick black dye sample sandwiched between two quartz glass. The spectra of black dye are presented in
8
Monitoring Metal Residues in Water
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H2T4-treated glass was used
to directly monitor metal residual concentration in treated solution,
thus avoiding porphyrin dispersion in water. Microscopic glass slides
(Forlabs; Carlo Erba, cut into 2.5 cm2 pieces) were sonicated
in water, isopropyl alcohol, and ultrapure water before use.
H2T4 deposition is achieved by dipping glass slides into H2T4 solutions
(10 μM) at pH 5.5 for 45 min: afterward glass slides were rinsed
with water to remove the excess of porphyrin. The amount of H2T4 deposited
on glass has been spectroscopically calculated by desorption experiments
(in sodium dodecyl sulfate solution at 10%w) and estimated
to be ∼3 × 10–7 M.
UV–visible
spectra were obtained on a JASCO V-560 UV–vis
spectrophotometer. All of the measurements were performed at room
temperature and under an atmospheric pressure. UV–vis spectra
of H2T4 deposited on glass were recorded from λ = 700 to 350
nm (data pitch 0.5 nm; band width 2.0 nm; scanning speed 100 nm/min)
before and after dipping (time ranging from 1 to 48 h) in the water
containing the residual Pb2+ and/or Zn2+ after
fibers PES–TiO2 treatment.
SEM analysis was
performed using a Field Emission Supra ZEISS VP
55 microscope equipped with an Oxford 10 mm2 SDD Detector
for energy-dispersive spectroscopy (EDS).
to directly monitor metal residual concentration in treated solution,
thus avoiding porphyrin dispersion in water. Microscopic glass slides
(Forlabs; Carlo Erba, cut into 2.5 cm2 pieces) were sonicated
in water, isopropyl alcohol, and ultrapure water before use.
H2T4 deposition is achieved by dipping glass slides into H2T4 solutions
(10 μM) at pH 5.5 for 45 min: afterward glass slides were rinsed
with water to remove the excess of porphyrin. The amount of H2T4 deposited
on glass has been spectroscopically calculated by desorption experiments
(in sodium dodecyl sulfate solution at 10%w) and estimated
to be ∼3 × 10–7 M.
UV–visible
spectra were obtained on a JASCO V-560 UV–vis
spectrophotometer. All of the measurements were performed at room
temperature and under an atmospheric pressure. UV–vis spectra
of H2T4 deposited on glass were recorded from λ = 700 to 350
nm (data pitch 0.5 nm; band width 2.0 nm; scanning speed 100 nm/min)
before and after dipping (time ranging from 1 to 48 h) in the water
containing the residual Pb2+ and/or Zn2+ after
fibers PES–TiO2 treatment.
SEM analysis was
performed using a Field Emission Supra ZEISS VP
55 microscope equipped with an Oxford 10 mm2 SDD Detector
for energy-dispersive spectroscopy (EDS).
9
Quantifying Cu2+ Binding to Engineered MBP Fusions
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A JASCO-V560 UV–Vis Spectrophotometer was used to quantitate Cu2+ ions incorporation into the MBP and cMBP1 to cMBP3 fusions. Briefly, an equal volume of purified-desalted periplasmic samples containing 1 × cOmplete protease inhibitor cocktail (Sigma-Aldrich, Gillingham, Dorset, UK) was mixed with a final concentration of 4 mM CuSO4.5H2O in a total sample volume of 1 ml. Samples were incubated overnight at room temperature and UV–Vis spectra of each was recorded at 190 to 900 nm wavelength. Additionally, cMBP2 and cMBP3 samples (also contain 1 × cOmplete protease inhibitor cocktail) were incubated overnight at room temperature with 0.1 to 10 mM CuSO4.5H2O and on the following day their UV–Vis spectra recorded. We used 10 mM copper sulphate as the highest concentration of Cu2+ because this is considerably more than the recognised EC50 toxic levels of copper in soils. Peak absorption values at 800 nm peaks (representing Cu2+ bound cMBP) were further analysed by well-known Michaelis–Menten and Allosteric Sigmoid kinetics in order to demonstrate Cu2+ ions cooperativity to cMBP2 and cMBP3 fusions.
10
Biofilm Characterization via SEM and EDX
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From each condition, tiles with biofilms attached were used for Scanning Electron Microscopy (SEM). Tiles were freeze dried and the images obtained using a Hitachi TM4000 plus (Hitachi, Japan) using an accelerating voltage of 15 kV [49 (link)]. Energy-dispersive X-ray Spectroscopy (EDX) was performed to evaluate the chemical composition of biofilms exposed to the control (0 mg/L GO) and 20 mg/L GO. Samples were deposited on a glass plate and coated with carbon for SEM analysis. The optical spectra were recorded using a Jasco V-560 UV–vis spectrophotometer; for the solid samples, the spectra were recorded in the diffuse reflectance mode using MgO as the reference.
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