Sc 9073

Manufactured by Santa Cruz Biotechnology

Sc-9073 is a lab equipment product offered by Santa Cruz Biotechnology. It is a versatile and reliable instrument designed for general laboratory applications. The core function of Sc-9073 is to provide consistent and accurate performance in a variety of laboratory tasks.

Automatically generated - may contain errors

Lab products found in correlation

Sc 23948, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Cell Signaling Technology (1 mentions) Sc 74442, by Santa Cruz Biotechnology (1 mentions) Sc 21870, by Santa Cruz Biotechnology (1 mentions) Ab68155, by Abcam (1 mentions) Ab16831, by Abcam (1 mentions) Ab9015, by Abcam (1 mentions) Ab209211, by Abcam (1 mentions) Ab59546, by Abcam (1 mentions) Ab21974, by Abcam (1 mentions) Sc 20357, by Santa Cruz Biotechnology (1 mentions) Ab9722, by Abcam (1 mentions) Sc 390715, by Santa Cruz Biotechnology (1 mentions) Sc 8468, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 9073

Protein Extraction and Western Blotting

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Protein extraction from cultured cells and isolated aortas was performed at 0°C to 4°C with a radioimmunoprecipitation assay buffer and protease inhibitor cocktail (Cell Signaling Technology). Twenty-microgram samples were separated by electrophoresis on 8% to 12% polyacrylamide gels in Laemmli buffer and transferred onto polyvinylidene difluoride membranes. Primary antibodies used for Western blotting included rabbit RANKL antibody (sc-9073; Santa Cruz Biotechnology), rabbit Mmp9 antibody (ab38898; Abcam), rabbit p-Jak2 antibody (sc-21870; Santa Cruz Biotechnology), rabbit JAK2 antibody (#3230; Cell Signaling Technology), rabbit p-signal transducer and activator of transcription 5 (Stat5) antibody (sc-11761; Santa Cruz Biotechnology), rabbit Stat5 antibody (sc-74442; Santa Cruz Biotechnology), and mouse α-tubulin antibody as a positive reference control (sc-23948; Santa Cruz Biotechnology). Primary antibodies were detected using a horseradish peroxidase-conjugated secondary antibody and visualized with an enhanced chemiluminescence kit (Thermo Fisher Scientific, Rockford, Ill).

Tanaka T., Kelly M., Takei Y, & Yamanouchi D. (2018). RANKL-mediated osteoclastogenic differentiation of macrophages in the abdominal aorta of angiotensin II-infused apolipoprotein E knockout mice. Journal of vascular surgery, 68(6 Suppl), 48S-59S.e1.

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Western Blot Analysis of Rat Knee Tissues

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Rat knee joint tissues were homogenized and lysed in a protein extraction reagent (Tissue protein extraction reagent, Pierce, Rockford, US). Western blot analysis was performed as described previously [29 (link)]. The primary antibodies to transferrin (17435-1-AP; Proteintech), catalase (ab209211; Abcam), SOD1 (ab16831; Abcam), SOD2 (ab68155; Abcam), SOD3 (ab21974; Abcam), glutathione peroxidase 1 (ab59546; Abcam), glutathione peroxidase 2 (MAB5470; R&D Systems), transthyretin (ab9015; Abcam), NF-κB (8242S; Cell Signaling), IL-1β (ab9722; Abcam), RANKL (sc-9073; Santa Cruz), and GAPDH (sc-20357; Santa Cruz) were obtained commercially. After extensive washing, appropriate secondary antibodies were used in the experiments, including anti-rabbit IgG antibody conjugated with horseradish peroxidase (7074S; Cell Signaling), anti-mouse IgG antibody conjugated with horseradish peroxidase (7076P2; Cell Signaling), or anti-sheep IgG antibody conjugated with horseradish peroxidase (313-035-003; Jackson ImmunoResearch).

Peng K.T., Tsai M.H., Lee C.W., Chiang Y.C., Chen P.C., Chen C.C., Chang C.H., Shih H.N, & Chang P.J. (2018). Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis. PLoS ONE, 13(8), e0202501.

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Proteomic Analysis of Periodontal Tissue

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Total proteins were extracted from periodontal tissue and analyzed with SDS-PAGE electrophoresis. Periodontal tissues were homogenized using RIPA buffer (10 μg/mL) and a protease inhibitor. Total proteins were isolated, and their concentrations were measured using the bicinchoninic acid (BCA) method. Proteins were then electrotransferred to the PVDF membrane, and the membrane containing the proteins was used for immunoblotting with required antibodies. Proteins were blocked with 5% non-fat milk in TBST buffer for 2 h, and they were then incubated with the primary antibodies (Santa Cruz Biotechnology; sc-8468, sc-9073, and sc-390715) at 4°C overnight. Proteins were subsequently incubated with secondary antibodies that were conjugated with horseradish peroxidase for 1 h at room temperature. GAPDH was used as an internal control. The images were analyzed using the Quantity One (version 4.62) software.

Li B., Zhang Y.H., Wang L.X., Li X, & Zhang X.D. (2015). Expression of OPG, RANKL, and RUNX2 in rabbit periodontium under orthodontic force. Genetics and molecular research : GMR, 14(4).

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