Four-week-old male KK and KK-Cg-Ay/J mice were obtained from the Jackson Laboratory (Bar Harbor, Maine, USA). Six-, 16-, and 42-week-old mice were used for analyses. Animals were housed in individual cages and fed lab chow ad libitum (LabDiet 5k52, St. Louis, MO, USA). The animals were housed in a room with a conditioned temperature (22–25°C), relative humidity (50–70%), and photoperiod (12-h light/12-h dark). This study was approved by the Institutional Animal Care and Use Committee (IACUC) of China Medical University (IACUC: 102–217).
Kk cg ay j mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
The KK-Cg-Ay/J mice are a genetically modified mouse strain. They carry a mutation in the Cg gene, which affects coat color, and the Ay allele, which influences obesity and diabetes-related traits. This mouse model is used in research to study these specific genetic characteristics.
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3 protocols using kk cg ay j mice
1
Mice Feeding and Housing Protocol
2
Mouse Strain Comparison for Diabetes
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Female C57BL/6 mice, 8–10 weeks old (Charles Rivers) were used for the majority of the study. For evaluation of a different mouse strain background, age-matched C3H/HeN mice were also tested. Nine to eleven week old KK.Cg-AY/J mice (Jackson Laboratories) were used as a model of type 2 diabetes.
3
CaCl2-Induced Carotid Artery Aneurysm
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C57BL/6 or KK.Cg‐Ay/J mice aged 10 weeks were obtained from the Jackson Laboratory (Bar Harbor, ME). The procedures for creating our modified CaCl2‐induced mouse model of aneurysm were described previously.13 We applied the procedure for the infrarenal aorta to the carotid artery in this study. Briefly, 0.5 mol/L CaCl2‐soaked gauze was applied perivascularly for 10 minutes to the carotid artery. The gauze was replaced with PBS‐soaked gauze for 5 minutes, and the incised area was sutured. The excess CaCl2 is converted into CaPO4, and the crystals act as adjuvants. After 1 to 4 weeks, the mice were sacrificed, and the carotid arteries were collected after fixing by perfusion with 4% paraformaldehyde. The diameter of the artery at the time of the initial surgery and sacrifice was measured with an electronic digital caliper (VWR International). All animal procedures were conducted in accordance with experimental protocols that were approved by the institutional animal care and use committee at the University of Wisconsin, Madison (protocol M02394).
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