Cellular malondialdehyde (MDA) levels were analyzed using the OxiSelect-thiobarbituric acid-reactive substance (TBARS) assay kit (Cell Biolabs, San Diego, CA, USA) following the manufacturer's instructions.
Oxiselect thiobarbituric acid reactive substance tbars assay kit
Manufactured by Cell Biolabs
Sourced in United States
The OxiSelect-thiobarbituric acid-reactive substance (TBARS) assay kit is a colorimetric assay that quantifies malondialdehyde (MDA), a marker of lipid peroxidation. The kit provides reagents and a protocol to measure MDA levels in biological samples.
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4 protocols using oxiselect thiobarbituric acid reactive substance tbars assay kit
1
Cellular Malondialdehyde Quantification
2
Determination of Brain MDA Levels
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The brain samples were homogenized in PBS (pH 7.4) including heparin and 1X BHT for the determination of MDA levels. The MDA in the brains was measured using the Oxiselect thiobarbituric acid-reactive substance (TBARS) assay kit containing thiobarbituric acid-reactive substances (Cell Biolabs, San Diego, CA, USA). Each sample was expressed as micromoles per mg protein according to the manufacturer’s instructions. The total protein concentration was measured before the MDA assay using the Pierce® BCA Protein Assay kit (Thermo, Rockford, USA).
3
Measuring Lipid Peroxidation in Serum and Mucosa
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Malondialdehyde content in serum and mucosa was measured using an OxiSelect thiobarbituric acid reactive substance (TBARS) Assay Kit (Cell Biolabs, Inc., San Diego, CA) as an index of lipid peroxidation (Kim et al., 2019 (link)). All the procedures followed the instruction of the manufacturer. Concentration of MDA in serum was expressed as micromoles per milliliter, and concentration of MDA in mucosal tissue was expressed as micromoles per milliliter of protein.
4
Lipid Peroxidation Measurement in Plasma and Tissue
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In order to determine the level of oxidative stress, lipid peroxidation product levels in plasma and left ventricular (LV) tissue collected at the time of sacrifice were measured by estimating the amount of malondialdehyde using the oxiselect thiobarbituric acid reactive substance (TBARS) assay kit (Cell Biolabs, San Diego, CA, USA) as described by us earlier by following the manufacturer’s instructions (18 (link)). Values were expressed as nmol/mL for plasma and nmol/mg protein for LV tissue.
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