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Ldh assay kit

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The LDH assay kit is a laboratory tool used to measure the activity of the enzyme lactate dehydrogenase (LDH) in biological samples. LDH is an important marker for various cellular processes and can be used to assess cell health and function.

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Lab products found in correlation

β gal assay kit, by Solarbio (3 mentions) Mrs agar, by Hopebio (2 mentions) Trypsin, by Solarbio (2 mentions) Bca protein assay kit, by Solarbio (2 mentions) Papain, by Solarbio (2 mentions) Mrs broth, by Hopebio (2 mentions) Microplate reader, by Thermo Fisher Scientific (2 mentions) Protamex, by Solarbio (1 mentions) Alcalase, by Solarbio (1 mentions) Neutrase, by Solarbio (1 mentions) Model 680, by Bio-Rad (1 mentions) Cck 8 assay, by Biosharp (1 mentions) Wheat germ agglutinin (wga), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Mk2206, by MedChemExpress (1 mentions) Hoechst 33342 reagent, by Thermo Fisher Scientific (1 mentions) Streptozotocin (stz), by Biosharp (1 mentions) Anti α tubulin antibody, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)

23 protocols using ldh assay kit

1

Bacterial Membrane Integrity Changes by Fe2+ Exposure

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Changes in bacterial membrane integrity following ferrous iron treatment were detected using an LDH assay kit (Solarbio). The S. aureus and E. coli precipitates (1 mL) at the logarithmic growth stage were obtained by centrifugation at 3 600 rpm for 5 min and diluted with H2O (450 μL). Then, 50 μL of Fe2+ (25, 50, and 200 µM) was mixed with S. aureus (450 μL) and E. coli (450 μL) at 37 °C for 30 min, respectively. The supernatant was collected by centrifugation at 3 600 rpm for 5 min at 4 °C. The LDH activity in the supernatant was then measured using an LDH assay kit (Solarbio).
Ma R., Fang L., Chen L., Wang X., Jiang J, & Gao L. (2022). Ferroptotic stress promotes macrophages against intracellular bacteria. Theranostics, 12(5), 2266-2289.
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2

Quantifying Cell Injury via LDH Assay

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Following the manufacturer's instructions, a lactate dehydrogenase (LDH) assay kit (Solarbio, Beijing, China; Cat. # BC0680) was used to detect the amount of LDH released by cells. The LDH absorbance was measured at 450 nm using a microplate reader (PerkinElmer). The experiment was independently repeated three times.
Tang D., Zheng Y., Wang G., Sheng C., Liu Z., Wang B., Zong Q., Zhang Y., Hou X., Yao M, & Zhou Z. (2023). HPV18 E6 inhibits α‐ketoglutarate‐induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway. FEBS Open Bio, 13(8), 1522-1535.
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3

Quantifying LDH Release in Cell Cultures

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LDH release in culture media was measured by using an LDH assay kit (SolarBio, Beijing, China) according to the manufacturer's instructions.
Zhu K., Guo J., Yu X., Wang Q., Yan C., Qiu Q., Tang W., Huang X., Mu H., Dou L., Bian Y., Han Q., Shen T., Li J, & Xiao C. (2021). Polypeptide Globular Adiponectin Ameliorates Hypoxia/Reoxygenation-Induced Cardiomyocyte Injury by Inhibiting Both Apoptosis and Necroptosis. Journal of Immunology Research, 2021, 1815098.
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4

Enzymatic Hydrolysis of Antarctic Krill

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Defatted Antarctic krill powder was provided by Zhejiang Hailisheng Biotechnology Co., Ltd. (Zhoushan, China), with a proximate composition as follows: moisture (8.29%), protein (78.33%), fat (1.17%), sugar (0.84%), and ash (4.62%). Neutrase (5 × 104 U/g), Alcalase (2 × 105 U/g), Papain (1 × 105 U/g), Trypsin (2.5 × 105 U/g), Protamex (1 × 105 U/g), the BCA protein assay kit, the β-GAL assay kit, and the LDH assay kit were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). L. rhamnosus ATCC7469 was provided by Shanghai Luwei Microbial Sci. &Tech. Co., Ltd. (Shanghai, China). MRS broth and MRS agar were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Tsingtao, China). The other reagents and chemicals were of analytical grade.
Liu Y., Yu X., Zhu Y., Yang W., Zeng Y., Hu Y, & Jiang W. (2022). Preparation, Characterization, and Mechanism of Antifreeze Peptides from Defatted Antarctic Krill (Euphausia superba) on Lactobacillus rhamnosus. Molecules, 27(9), 2771.
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5

Measuring Lactate Dehydrogenase Levels

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The lactate dehydrogenase (LDH) concentration was measured using an LDH assay kit according to the manufacturer's manual (Solarbio, Beijing, China) by a routine microtitre plate reader (wavelength: 572 nm).
Yu X., Ruan Y., Shen T., Qiu Q., Yan M., Sun S., Dou L., Huang X., Wang Q., Zhang X., Man Y., Tang W., Jin Z, & Li J. (2020). Dexrazoxane Protects Cardiomyocyte from Doxorubicin-Induced Apoptosis by Modulating miR-17-5p. BioMed Research International, 2020, 5107193.
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6

LDH Assay for Cell Cytotoxicity

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The cell supernatants were detected using an LDH assay kit (Solarbio Ltd., Beijing, China) according to the manufacturer’s instructions. The LDH data were measured at 450 nm wavelength using a microplate reader (Model 680, Bio-Rad, St. Louis, MO, USA) and were expressed as U/104 cells. Each sample was assayed three times.
Gao T., Wang Z., Dong Y., Cao J, & Chen Y. (2021). Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice. International Journal of Molecular Sciences, 22(21), 11894.
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7

LDH Activity Assay for Cellular Injury

Cited 1 time
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The release of LDH activity was determined by means of LDH assay kit (Solarbio, Beijing, China) for assessing the cell injury in H9c2 cells [20 (link)]. Cells were centrifuged at 8000 g for 10 min at 4°C and the supernatant was collected on ice for testing. 200 μL of LDH reagent solution was added in line with the instructions provided by vendor. Finally, the measurement of absorbance was performed at 450 nm with a spectrophotometer (Mapada, Shanghai, China) which had been preheated for 30 min.
Lu Z., Liu Z, & Fang B. (2022). Propofol protects cardiomyocytes from doxorubicin-induced toxic injury by activating the nuclear factor erythroid 2-related factor 2/glutathione peroxidase 4 signaling pathways. Bioengineered, 13(4), 9145-9155.
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8

Quantifying Cellular LDH Activity

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Lactate dehydrogenase (LDH) activity was measured by using a LDH assay kit according to the manufacturer's protocol (Solarbio). Cells were lysed by an ultrasonic homogenizer and then centrifuged at 8000g at 4°C for 10 minutes. The LDH activity of cell supernatant was analyzed by calculating the relative absorbance at 490 nm. The OD values of the experimental group were normalized to those of the control group.
Gu M., Wang Z., Feng F., Yang Y., Sun X, & Yang D. (2022). Inhibition of PIKfyve Ameliorates the Proliferation and Migration of Vascular Smooth Muscle Cells and Vascular Intima Hyperplasia By Reducing mTORC1 Activity. Journal of Cardiovascular Pharmacology, 79(5), 739-748.
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9

Mouse Serum LDH Activity Quantification

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The activity of lactate dehydrogenase (LDH) in mouse serum was assessed using an LDH Assay Kit (Solarbio), following the manufacturer’s instructions. The OD was measured at a wavelength of 450 nm with the microplate reader. The LDH activity was determined by comparing the OD values of the test samples with a standard curve generated from known LDH concentrations.
Niu X., Zhang J., Hu S., Dang W., Wang K, & Bai M. (2024). lncRNA Oip5-as1 inhibits excessive mitochondrial fission in myocardial ischemia/reperfusion injury by modulating DRP1 phosphorylation. Cellular & Molecular Biology Letters, 29, 72.
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10

LDH Release Assay Protocol

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Following the indicated treatments, the release of LDH was determined using an LDH assay kit (Solarbio) according to the manufacturer’s protocol.
Di M., Miao J., Pan Q., Wu Z., Chen B., Wang M., Zhao J., Huang H., Bai J., Wang Q., Tang Y., Li Y., He J., Xiang T., Weng D., Wang L., Xia J, & Zhao C. (2022). OTUD4-mediated GSDME deubiquitination enhances radiosensitivity in nasopharyngeal carcinoma by inducing pyroptosis. Journal of Experimental & Clinical Cancer Research : CR, 41, 328.
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