Adiponectin cre adipoq cre mice

All mouse care and procedures were performed in accordance with animal experimental protocols approved by the Northwestern University Institutional Animal Care and Use Committee (IACUC). All mice were housed on a 14-hr/10-hr light/dark cycle with ad libitum access to chow diet and water. GFP-LC3 mice, Becn1^F121A KI mice, and Becn1^+/− KO mice were described previously (Mizushima et al., 2004 (link); Qu et al., 2003 (link); Rocchi et al., 2017 (link)). C57BL/6J mice (JAX#000664), Adiponectin-Cre (Adipoq-Cre) mice (JAX#028020), and Adiponectin KO (Adipoq KO) mice (JAX#008195) were acquired from the Jackson Laboratory. All strains have been backcrossed for more than 12 generations to C57BL/6J mice. All experiments were performed with sex- and age-matched mice. Diet-induced diabetic mice were generated by HFD (D12492; Research Diets Inc) feeding for 12 weeks. SBI-0206965 (2 mg/kg body weight), Spautin-1 (2 mg/kg body weight) and chloroquine (50 mg/kg body weight) were injected intraperitoneally (i.p.) into mice for 10 consecutive days.