Anti rad51 antibody

IHC was performed with a normal inverted microscope (NION, Japan) by using an anti-YAP1 antibody (1:200; Cell Signaling Technology), anti-CPNE3 antibody (1:50; abcam, Proteintech), anti-cysteine-rich angiogenic inducer 61 (CYR61) antibody (1:100; Proteintech), and anti-RAD51 antibody (1:100; Proteintech). IHC was performed as described previously [11 (link)]. Two pathologists assessed the staining results based on the proportion of positively stained cells and the staining strength.

The CHK1 inhibitor Rabusertib was purchased from MedChemExpress. Sheep polyclonal anti‐BubR1 antibody, rabbit monoclonal anti‐γ‐H2A.X and anti‐MAP1LC3A antibodies were obtained from Abcam. The anti‐α‐tubulin‐FITC antibody and Hoechst 33342 were purchased from Sigma. AlexaFluor 488 goat anti‐rabbit antibody and AlexaFluor 594 goat anti‐rabbit antibody were obtained from Invitrogen. The human anti‐centromere CREST antibody was purchased from Fitzgerald Industries International. Mouse polyclonal anti‐BAX, rabbit polyclonal anti‐γ‐H2A.X antibody and anti‐Rad51 antibody were purchased from Proteintech. Rabbit monoclonal anti‐α‐tubulin antibody and phospho‐Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) were purchased from Cell Signaling Technology. FITC‐conjugated and TRITC‐conjugated goat anti‐rabbit IgG, TRITC‐conjugated goat anti‐mouse IgG and TRITC‐conjugated donkey anti‐human IgG were obtained from Zhongshan Golden Bridge Biotechnology, Co., Ltd. All other chemicals and reagents were from Sigma‐Aldrich Corp.