Purified active Cdk1/cyclin B1 was purchased from Millipore. Phosphocellulose P81 paper discs were purchased from Whatman. [γ-32P]ATP (250 µCi at 10 µCi/µl) was obtained from Perkin-Elmer. Precast 16.5% acrylamide Tris/Tricine gels, in an 18-well format, and peptide molecular mass standards were purchased from Bio-Rad. Lambda protein phosphatase was obtained from New England BioLabs. Other chemicals were obtained from Sigma.
Phosphocellulose p81 paper discs
Manufactured by Cytiva
Phosphocellulose P81 paper discs are a type of laboratory filter paper. They are made of phosphocellulose and are commonly used in biochemical assays for the separation and purification of various biomolecules such as proteins, peptides, and nucleic acids.
Automatically generated - may contain errors
2 protocols using phosphocellulose p81 paper discs
1
Cdk1/cyclin B1 Kinase Assay
2
Cdk1/cyclin B1 Kinase Assay
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