Pe rat anti mouse cd62l

Manufactured by BD

Sourced in United States

The PE rat anti-mouse CD62L is a laboratory reagent used for the detection and analysis of the CD62L (L-selectin) molecule, which is expressed on the surface of certain immune cells, such as lymphocytes. This reagent is a fluorescently labeled antibody that binds specifically to the CD62L antigen, allowing for its identification and quantification in various experimental and analytical applications.

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Lab products found in correlation

Apc rat anti mouse cd8a, by BD (1 mentions) Fitc rat anti mouse cd44, by BD (1 mentions) Anti cd44 apc, by BD (1 mentions) Anti cd4 apc, by BD (1 mentions) Anti nk1 1 percp cy5, by BD (1 mentions) Anti cd8 fitc, by BD (1 mentions) Anti cd44 pe, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Anti cd3 efluor 450, by Thermo Fisher Scientific (1 mentions) Anti cd8 percp cy5, by BD (1 mentions) Anti cd4 pe, by BD (1 mentions)

2 protocols using pe rat anti mouse cd62l

Lycorine Hydrochloride Immunomodulation Assay

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Lycorine hydrochloride (Figure 1A) was obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). The anti-mouse CTLA-4 (clone 9H10) monoclonal antibody was purchased from BioXCell (West Lebanon, NH, USA). Primary antibodies against cyclin D1, cyclin D3, CDK2, and β-actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Purified PE rat anti-mouse CD4, FITC rat anti-mouse Foxp3, APC rat anti-mouse CD8a, PE rat anti-mouse CD62L, and FITC rat anti-mouse CD44 were purchased from BD Biosciences, Inc. (San Jose, CA, USA).

Li X., Xu P., Wang C., Xu N., Xu A., Xu Y., Sadahira T., Araki M., Wada K., Matsuura E., Watanabe M., Zheng J., Sun P., Huang P., Nasu Y, & Liu C. (2017). Synergistic effects of the immune checkpoint inhibitor CTLA-4 combined with the growth inhibitor lycorine in a mouse model of renal cell carcinoma. Oncotarget, 8(13), 21177-21186.

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Multiparametric Flow Cytometry Analysis

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Single cell suspensions from the lymph nodes (LNs) and Spleen were processed as described [32 ]. Antibodies used in this study were: anti-CD3-eFluor 450 (eBioscience, 48–0032-82), anti-CD8-FITC (BD Biosciences, cat #553030), anti-CD8-PerCP-Cy5.5 (BD Biosciences, cat #561109), anti-CD4-APC (BD Biosciences, cat #553051), anti- CD4-PE (BD Biosciences, cat #553730), anti-NK1.1-PerCP-Cy5.5 (BD Biosciences, cat #561111), anti-CD44-APC (BD Biosciences, cat #559250), anti-CD44-PE (BD Biosciences, cat #553133), PE-rat anti mouse CD62L (BD Biosciences, cat #553151; eFluor450-rat anti mouse IFNγ (eBioscience, cat #48–7311-82), PE-rat anti-mouse IL-6 (BD Biosciences, cat #: 562050) and APC-rat anti-mouse TNF-α (BD Biosciences, cat #: 561062). Single-cell suspensions were analyzed on BDTM LSR II flow cytometer (BD Biosciences, San Jose, California) and data was analyzed using FlowJo version 10 software (Tree Star, Ashland, Oregon).

Clark S.M., Song C., Li X., Keegan A.D, & Tonelli L.H. (2018). CD8+ T Cells Promote Cytokine Responses to Stress. Cytokine, 113, 256-264.

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