For time‐lapse imaging, an inverted automated microscope from Nikon (Nikon Eclipse Ti2, Nikon, GmbH, Germany) equipped with temperature incubator (Cage incubator, OKO Touch, Okolab S.R.L., Italy) was used. The microfluidic device was mounted in an in‐house fabricated chip holder and placed inside the incubator. Additionally, the setup was equipped with a 100× oil objective (CFI P‐Apo DM Lambda 100× Oil, Nikon GmbH, Germany), DS‐Qi2 camera (Nikon camera DS‐Qi2, Nikon GmbH, Germany), and an automated focus system (Nikon PFS, Nikon GmbH, Germany) to compensate thermal drift during long‐term microscopy. Eighty cultivation chambers were selected manually for each experiment and were monitored using the NIS‐Elements Imaging Software (Nikon NIS Elements AR software package, Nikon GmbH, Germany). Time‐lapse images were recorded every 5 min.
Cfi p apo dm lambda 100 oil
Manufactured by Nikon
Sourced in Germany
The CFI P-Apo DM Lambda 100× Oil is a microscope objective lens manufactured by Nikon. It is designed to provide high-resolution imaging with a 100× magnification. The lens is optimized for use with oil immersion techniques, which can improve image quality and resolution.
Automatically generated - may contain errors
3 protocols using cfi p apo dm lambda 100 oil
1
Time-lapse Imaging of Microfluidic Cultures
2
Live-Cell Microscopy of Microbial Cultures
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Live-cell microscopy was conducted using an inverted automated microscope (Nikon Eclipse Ti2, Nikon, Germany). The microscope was placed in a cage incubator for optimal temperature control at 30°C (Cage incubator, OKO Touch, Okolab S.R.L., Italy). The microfluidic chip was attached to a holder manufactured in-house. The setup was equipped with a 100 × oil objective (CFI P-Apo DM Lambda 100 × Oil, Nikon GmbH, Germany), a DS-Qi2 camera, and an automatic focusing system (Nikon PFS, Nikon GmbH, Germany) to prevent thermal drift during cultivation. One hundred cultivation chambers were manually selected in each experiment by using NIS Elements software (Nikon NIS Elements AR software package, Nikon GmbH, Germany). Images were taken every 10 min with an exposure time of 50 ms.
3
Live-Cell Imaging with Automated Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Live-cell imaging was conducted using a Nikon inverted automated microscope (Nikon Eclipse Ti2, Nikon, Germany). The microscope stage was located in a cage incubator for optimal temperature control (Cage incubator, OKO Touch, Okolab S.R.L., Italy). Inside the cage incubator, the microfluidic device was positioned in an in-house fabricated chip holder. In addition, the setup was provided with a 100× oil objective (CFI P-Apo DM Lambda 100× Oil, Nikon GmbH, Germany), a DS-Qi2 camera (Nikon camera DSQi2, Nikon GmbH, Germany)
and an automated focus system (Nikon PFS, Nikon GmbH, Germany) to compensate for thermal drift during long-term microscopy. Eighty cultivation chambers were manually chosen for each experiment and were managed with NIS-Elements Imaging Software (Nikon NIS Elements AR software package, Nikon GmbH, Germany). Pressure-driven pumps (LineUp, Fluigent, Jena, Germany) were used for the generation of different flow profiles. The flow profiles were generated with a specifically tailored cultivation profile implemented in a pumpspecific software tool (microfluidic automation tool (MAT), Fluigent, Jena, Germany).
and an automated focus system (Nikon PFS, Nikon GmbH, Germany) to compensate for thermal drift during long-term microscopy. Eighty cultivation chambers were manually chosen for each experiment and were managed with NIS-Elements Imaging Software (Nikon NIS Elements AR software package, Nikon GmbH, Germany). Pressure-driven pumps (LineUp, Fluigent, Jena, Germany) were used for the generation of different flow profiles. The flow profiles were generated with a specifically tailored cultivation profile implemented in a pumpspecific software tool (microfluidic automation tool (MAT), Fluigent, Jena, Germany).
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