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Verikine hs mouse interferon beta serum elisa kit

Manufactured by PBL Assay Science
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The VeriKine-HS Mouse Interferon Beta Serum ELISA Kit is a laboratory assay used to detect and quantify mouse interferon beta levels in serum samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) format to provide a sensitive and specific measurement of the target analyte.

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Lab products found in correlation

Mouse cxcl10 quantikine elisa kit, by R&D Systems (2 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Biotinylated detection abs, by R&D Systems (1 mentions) Il 6 quantikine elisa, by R&D Systems (1 mentions) Human ifn beta elisa kit, by PBL Assay Science (1 mentions)

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VeriKine Mouse Interferon Beta ELISA Kit Mouse interferon beta

10 protocols using verikine hs mouse interferon beta serum elisa kit

1

Bone Marrow-Derived Macrophage Stimulation

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Bone Marrow Derived Macrophages (BMDM) were generated in vitro by flushing bone marrow from both femurs and tibias. Cells were cultured for 6 days in medium that induces the differentiation of macrophages (DMEM supplemented with 10% FBS, 1% sodium pyruvate, 1% L-glutamine, 1% penicillin/streptomycin, 2-mercaptoethanol, and 10% L-cell conditioned media). 5 mL of fresh media was added at day 3. At day 6 BMDMs were plated at 1 × 106 cells in 1 ml in 12 well plates. The next day the cells were stimulated with 10 μg/ml CDG or 2′3′ cGAMP or 100 ng/ml LPS (Sigma, MO.) for 5 h. Supernatants were harvested and IFNβ was determined by ELISA (VeriKine-HS Mouse Interferon Beta Serum ELISA Kit, PBL Assay Science, NJ).
Walker M.M., Kim S., Crisler W.J., Nguyen K., Lenz L.L., Cambier J.C, & Getahun A. (2020). Selective Loss of Responsiveness to Exogenous but Not Endogenous Cyclic-Dinucleotides in Mice Expressing STING-R231H. Frontiers in Immunology, 11, 238.
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2

Quantifying Mouse Serum and Cell IFNβ

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Mouse serum IFNβ and culture medium IFNβ were measured by using VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) according to the manufacturer’s instruction.
Xia Y., Inoue K., Du Y., Baker S.J., Reddy E.P., Greenblatt M.B, & Zhao B. (2022). TGFβ reprograms TNF stimulation of macrophages towards a non-canonical pathway driving inflammatory osteoclastogenesis. Nature Communications, 13, 3920.
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3

IFN-β Secretion Assay in BMDMs

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BMDMs on day 6 of differentiation were treated with the indicated concentrations of drugs in a controlled volume of culture medium for 16 to 24 hours. Culture medium was harvested and was assessed for IFN-β concentration using the VeriKine-HS Mouse Interferon Beta Serum ELISA kit (PBL Assay Science) according to the manufacturer’s instructions.
Mottis A., Li T.Y., El Alam G., Rapin A., Katsyuba E., Liaskos D., D’Amico D., Harris N.L., Grier M.C., Mouchiroud L., Nelson M.L, & Auwerx J. (2022). Tetracycline-induced mitohormesis mediates disease tolerance against influenza. The Journal of Clinical Investigation, 132(17), e151540.
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4

Bifidobacterium Augments DC-Mediated IFNβ Production

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Bone marrow–derived DCs (2 × 106) were cocultured with MC38 cells (2 × 106) with 10 µg/ml anti-CD47 or 10 µg/ml rat IgG in the presence or absence of 2 × 106Bifidobacterium for 8 h. DCs were then sorted out for Ifnb1 quantification by qPCR. In some experiments, 2 × 106 bone marrow–derived DCs were cocultured with 2 × 106Bifidobacterium, heated-killed Bifidobacterium, or Bifidobacterium culture supernatants (1:10 diluted), respectively. The resulting supernatants were collected 16 h later, followed by quantification of IFNβ concentration with VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) in accordance with the manufacturer’s instructions.
Shi Y., Zheng W., Yang K., Harris K.G., Ni K., Xue L., Lin W., Chang E.B., Weichselbaum R.R, & Fu Y.X. (2020). Intratumoral accumulation of gut microbiota facilitates CD47-based immunotherapy via STING signaling. The Journal of Experimental Medicine, 217(5), e20192282.
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5

Measurement of Interferon-β and CXCL10

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Tumor tissues were homogenized in PBS with protease inhibitor followed by addition of Triton X-100. Cell culture supernatants were obtained from isolated CD11c+ cells after 48 hr-incubation with fresh GM-CSF. The concentration of IFN-β and CXCL10 was measured with VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and mouse CXCL10 Quantikine ELISA kit (R&D) in accordance with the manufacturer’s instructions, respectively.
Hou Y., Liang H., Rao E., Zheng W., Huang X., Deng L., Zhang Y., Yu X., Xu M., Mauceri H., Arina A., Weichselbaum R.R, & Fu Y.X. (2018). Non-canonical NF-κB Antagonizes STING Sensor-Mediated DNA Sensing in Radiotherapy. Immunity, 49(3), 490-503.e4.
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6

Mouse IFN-β Serum Quantification

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Mouse serum IFN-β was measured by using VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) according to the manufacturer’s instruction.
Inoue K., Deng Z., Chen Y., Giannopoulou E., Xu R., Gong S., Greenblatt M.B., Mangala L.S., Lopez-Berestein G., Kirsch D.G., Sood A.K., Zhao L, & Zhao B. (2018). Bone protection by inhibition of microRNA-182. Nature Communications, 9, 4108.
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7

Quantitative Cytokine ELISA Protocol

Cited 1 time
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Concentrations of IL-6, IL-12, and TNFα in culture supernatants or mouse sera were determined by quantitative ELISA using cytokine-specific coating Abs and biotinylated detection Abs (eBioscience) as previously described (10 (link), 22 (link)). Mouse serum levels of IL-10 and IL-1β were analyzed by ELISA using cytokine-specific coating Abs and biotinylated detection Abs (R&D) as previously described (10 (link), 22 (link)). Levels of IFN-β in mouse sera were measured using the VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science, Piscataway, NJ) following the manufacturer’s protocol.
Lalani A.I., Moore C.R., Luo C., Kreider B.Z., Liu Y., Morse HC I.I.I, & Xie P. (2014). Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. Journal of immunology (Baltimore, Md. : 1950), 194(1), 334-348.
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8

Measuring Inflammatory Cytokine Secretion

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The secretion of the pro-inflammatory proteins interferon beta (IFNβ, VeriKine-HS Mouse Interferon Beta Serum ELISA Kit, PBL Assay Science, Piscataway, NJ, USA) and interleukin-6 (IL-6, Quantikine IL-6 ELISA, R&D Systems, Minneapolis, MN, USA), into the medium was measured 4 hours after poly(I:C) transfection according to the manufacturer’s instructions.
Sales Conniff A., Encalada G., Patel S., Bhandary M., Al-Takrouri F, & Heller L. (2022). Poly(I:C) transfection induces a pro-inflammatory cascade in murine mammary carcinoma and fibrosarcoma cells. RNA Biology, 19(1), 841-851.
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9

Radiation-Induced Tumor Cytokine Analysis

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Tumor tissues were excised on day 3 after radiation and homogenized in PBS with protease inhibitor. After homogenization, Triton X-100 was added to obtain lysates. Cell culture supernatants were obtained from isolated CD11c+ cells after 48h-incubation with fresh GM-CSF. The concentration of IFN-β and CXCL10 was measured with VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and mouse CXCL10 Quantikine ELISA kit (R&D) in accordance with the manufacturer's instructions, respectively.
Deng L., Liang H., Xu M., Yang X., Burnette B., Arina A., Li X.D., Mauceri H., Beckett M., Darga T., Huang X., Gajewski T.F., Chen Z.J., Fu Y.X, & Weichselbaum R.R. (2014). STING-dependent Cytosolic DNA Sensing Promotes Radiation-induced Type I interferon-dependent Antitumor Immunity in Immunogenic Tumors. Immunity, 41(5), 843-852.
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10

Interferon Beta Secretion Assay

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Primary MEFs and human tumor cell lines were seeded in 96-well plates at a density of 15,000 cells/well. Approximately 15 hours post-seeding, cells were exposed to IR. Cell culture supernatant were harvested 48 hours post-IR and assayed for IFN-beta using the VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and the Human IFN-beta ELISA kit for murine and human cell lines following the manufacturer's protocol. Absorbance at 450 nm was measured using a BioTek Synergy HT plate reader. IFN-beta concentration was calculated by using standard concentration and fitting a five-parameter logistic non-linear regression model available at the free analysis software ELISA Analysis (http://www.elisaanalysis.com/).
Ranoa D.R., Parekh A.D., Pitroda S.P., Huang X., Darga T., Wong A.C., Huang L., Andrade J., Staley J.P., Satoh T., Akira S., Weichselbaum R.R, & Khodarev N.N. (2016). Cancer therapies activate RIG-I-like receptor pathway through endogenous non-coding RNAs. Oncotarget, 7(18), 26496-26515.
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