Dulbecco modified eagle medium (DMEM) medium and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Insulin, 3-isobutylmethylxanthine (IBMX), indomethacin, 3,3′,5-triiodo-L-thyronine (T3), dexamethasone, troglitazone, Oil-red O powder, and CL316,243 were obtained from Sigma Aldrich (St Louis, MO, USA). Poly-vinylidene difluoride (PVDF) was procured from Millipore (Merck KGaA, Darmstadt, Germany). The electrochemiluminescence (ECL) kit was obtained from GE Healthcare Life Sciences (Seoul, Korea).
Dulbecco modified eagle medium medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
Dulbecco modified eagle medium (DMEM) is a cell culture medium formulation developed by Harry Eagle. It is commonly used to support the growth of various mammalian cell lines in vitro. DMEM contains a balanced salt solution, amino acids, vitamins, and other nutrients required for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene difluoride pvdf, by Merck Group (2 mentions)
Electrochemiluminescence ecl kit, by GE Healthcare (2 mentions)
Insulin, by Merck Group (2 mentions)
Dexamethasone (dex), by Merck Group (2 mentions)
Cl316 243, by Merck Group (1 mentions)
3 isobutyl methylxanthine ibmx, by Merck Group (1 mentions)
3 3 5 triiodo l thyronine t3, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Troglitazone, by Merck Group (1 mentions)
Oil red o powder, by Merck Group (1 mentions)
3 isobutylmethylxanthine, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
T75 tissue culture flask, by Greiner (1 mentions)
Hek293t cell line, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hek293t cell line, by American Type Culture Collection (1 mentions)
Raji cell line, by American Type Culture Collection (1 mentions)
4 protocols using dulbecco modified eagle medium medium
1
Adipocyte Differentiation Protocol
2
Adipogenic Differentiation Assay
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VA was purchased from Sigma Chemicals Co. (St. Louis, MO, USA). VA was dissolved in dimethyl sulfoxide (DMSO). Dulbecco modified eagle medium (DMEM) medium and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Insulin, 3-isobutylmethylxanthine (IBMX), and dexamethasone were obtained from Sigma-Aldrich (St Louis, MO, USA). Poly-vinylidene difluoride (PVDF) was procured from Millipore (Merck KGaA, Darmstadt, Germany). The electrochemiluminescence (ECL) kit was obtained from GE Healthcare Life Sciences (Seoul, Korea).
3
Culturing Canine and Human Lymphoma Cell Lines
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The canine B-cell lymphoma cell line CLBL-1 was provided by Dr. Barbara Rütgen (University of Vienna, Austria). The human Burkitt's lymphoma Raji cell line and the human cell line HEK 293 T cell line (appropriated for ectopic expression of mammalian proteins) were obtained from the American Type Culture Collection (ATCC, Manassas, VA). CLBL-1 and Raji cell lines were maintained in RPMI-1640 medium (Gibco, Life Technologies, Paisley, UK) supplemented with 10% FCS (Gibco) and penicillin 100 U/ml/streptomycin 0.1 mg/mL (Gibco). HEK 293 T cell line was cultured in Dulbecco Modified Eagle Medium (DMEM) medium supplemented with 10% FCS (Gibco) and penicillin 100 U/mL/streptomycin 0.1 mg/mL (Gibco). All cell lines cultures were maintained at 37 °C in a humidified atmosphere of 5% CO2 (T75-tissue culture flasks, Greiner Bio-One, Kremsmünster, Austria).
4
Fucoidan from U. pinnatifida: Composition, Cellular Effects
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HMWF were derived from U. pinnatifida and prepared by Haerim Fucoidan Co., Ltd. (Wando, Republic of Korea). HMWF’s chemical composition was originally reported to contain fucose (21%), galactose (23%), mannose (0.9%), uronic acid (10.9%), sulfate (30.9%), and a peak molecular weight of 258.7 kDa as determined by high performance gel permeation chromatography [23 (link)]. For the cell culture, Dulbecco Modified Eagle Medium (DMEM) medium was purchased from GIBCO (Brooklyn, NY, USA) and fetal bovine serum, trypsin-EDTA, and penicillin-streptomycin were purchased from Hyclone (Logan, UT, USA). MTT was purchased from Sigma-Aldrich (St. Louis, MO, USA). Monoclonal primary antibodies, which include anti-claudin-1, anti-occludin, anti-RAGE, anti-COX-2, anti-iNOS, anti-NF-κB anti-p-NF-κB and anti-GAPDH, as well as secondary antibodies such as anti-mouse, anti-rabbit and anti-rat, were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Anti-ZO-1 antibody was purchased from Cell Signaling Technology Inc. (Denvers, MA, USA). MG-H1 was purchased from Iris Biotech (Marktredwitz, Germany).
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