To observe the effect of hypoxia (physical hypoxia) on the angiogenic ability of ADSCs, they were cultured on Matrigel matrix gel (BD356234) using EGM-2 culture medium (Lonza, Walkersville, MD, USA), resuspended in EGM-2 culture medium, and inoculated into Matrigel matrix gel-treated 24-well plates containing 8 × 104 cells/well. The cells were placed in a hypoxic or normoxic incubator and imaged under a microscope after 3 h.
Matrigel matrix gel
Manufactured by Lonza
Sourced in United States
Matrigel matrix gel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is used as a substrate for the in vitro cultivation of cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using matrigel matrix gel
1
Hypoxia Impacts ADSC Angiogenesis
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