Early log phase cells were seeded at 5 × 103 per well in 96-well plates for the MTT assay. Cell density was measured by using Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA) following the manufacturer's instructions. The absorbance value (OD) of each sample was read at wavelength of 570 nm in a microtiter plate reader (Promega, Fitchburg, WI, USA). The measured absorbance of converted dye is proportional to cell viability [46 (link)]. All experiments were repeated at least three times.
Cell viability kit
Manufactured by Roche
Sourced in United States
The Cell Viability Kit is a laboratory instrument designed to assess the viability of cells. It provides a quantitative measure of the number of living cells in a sample.
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Lab products found in correlation
8 protocols using cell viability kit
1
MTT Assay for Cell Viability
2
Cell Viability Assay with Inhibitors
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2,000 cells were plated in triplicates in each well of a 96-well plate, and 3-day MTT assays were performed using the Cell viability Kit (Roche, 11465007001) per the manufacturer’s protocol. Inhibitor pretreatment was given for 1 hour, followed by cotreatment of the cells with inhibitors with or without DC661. Nonlinear regression (curve fit) method was used to calculate IC50.
3
MTT Cell Viability Assay Protocol
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2 × 103 cells were seeded in 96‐well plates for the MTT assay. Cell density was measured following the instruction of Cell Viability Kit (MTT, Roche, Indianapolis, IN). The absorbance value (OD) was got in a microtitre plate reader at wavelength of 570 nm. All experiments were repeated for three times.
4
Breast Cancer Cell Migration Assay
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To evaluate breast cancer cell migration, 8 nm pore-size culture inserts (Transwell, Costar) were placed into the wells of a 24-well culture plate, separating the upper and the lower chambers. 1x 10^4 control or AMPH-1 knockdown cells were seeded to the upper chamber in 200ul of serum-free medium. After 12 h of incubation at 37 °C in 5% CO2, the number of cells that migrated through the pores was counted in 3 independent visual fields. The cell morphology was observed by hematoxylin staining. The assay was performed in triplicate.
For MTT assay, 5×103 early log phase of control or AMPH-1 knockdown cells were seeded in 96-well plates for the MTT assay. Cell density was measured by Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA) following the manufacturer's instructions. The absorbance value (OD) was read in a microtiter plate reader at wavelength of 570 nm. All experiments were repeated at least three times.
For colony formation assay, cells were seeded into 96 well dishes with density of 5×104 cells per well. Individual colonies were fixed and stained with a solution containing 0.2% crystal violet in 10% ethanol for 30 minutes. The amount of dye taken up by the monolayer is quantitated in a spectrophotometer or plate reader at 570 nm.
For MTT assay, 5×103 early log phase of control or AMPH-1 knockdown cells were seeded in 96-well plates for the MTT assay. Cell density was measured by Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA) following the manufacturer's instructions. The absorbance value (OD) was read in a microtiter plate reader at wavelength of 570 nm. All experiments were repeated at least three times.
For colony formation assay, cells were seeded into 96 well dishes with density of 5×104 cells per well. Individual colonies were fixed and stained with a solution containing 0.2% crystal violet in 10% ethanol for 30 minutes. The amount of dye taken up by the monolayer is quantitated in a spectrophotometer or plate reader at 570 nm.
5
Cell Viability Assay for Sphingolipid/Cholesterol Pathway
6
Cell Growth Analysis and Colony Formation
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In our study, cell growth status was analyzed by two methods, the Celigo imaging cytometry system and MTT assays. The fluorescence intensity of cells was scanned and the number of cells was automatically calculated by Celigo imaging cytometry system (Nexcelom, Lawrence, MA, USA). Cell viability was measured with a Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA) according to the manufacturer's instructions. The absorption of the solution was measured at 570 nm at various time points.
Colony formation assays were performed to assess the ability of a single cell to grow into a colony. Cells were plated at a low density (500-1000 cells/well) onto 6 well plates and observed for 2 weeks. Colonies were fixed in 4% paraformaldehyde, stained with 0.5% crystal violet staining solution and washed with PBS.
Colony formation assays were performed to assess the ability of a single cell to grow into a colony. Cells were plated at a low density (500-1000 cells/well) onto 6 well plates and observed for 2 weeks. Colonies were fixed in 4% paraformaldehyde, stained with 0.5% crystal violet staining solution and washed with PBS.
7
Cell Viability Assay Using MTT
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For assay of cell growth, cells were seeded at 5 × 10 3 per well into a 96 well-plate and subjected to a Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA), according to the instruction of the manufacturer. The MTT assay is a colorimetric assay for assessing viable cell number, taking advantage that NADPH-dependent cellular oxidoreductase enzymes in viable cells reduce the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble formazan in purple readily being quantified by absorbance value (OD) at 570 nm in a microtiter plate reader (Promega, Fitchburg, WI, USA). Experiments were performed 5 times.
8
Cell Growth Assay Protocol
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For assay of cell growth, cells were seeded into 24 well-plate at 1X10 4 cells per well in the conditioned media and subjected to a Cell Viability Kit (MTT, Roche, Indianapolis, IN, USA), according to the instruction of the manufacturer.
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