Ubiquinone 10

Manufactured by Merck Group

Ubiquinone-10 is a naturally occurring coenzyme found in the mitochondria of cells. It plays a crucial role in the electron transport chain, which is responsible for the production of cellular energy in the form of ATP. Ubiquinone-10 facilitates the transfer of electrons between the various complexes in the chain, enabling efficient energy generation.

Automatically generated - may contain errors

Lab products found in correlation

α tocopherol, by Merck Group (1 mentions) Sodium borohydride, by Carl Roth (1 mentions) Chloroform, by Carl Roth (1 mentions) 1 palmitoyl 2 oleoyl phosphatidylglycerol popg, by Avanti Polar Lipids (1 mentions) Mini protean tgx precast gel, by Bio-Rad (1 mentions) Chlorpromazine, by Merck Group (1 mentions) Ni nta agarose resin, by Thermo Fisher Scientific (1 mentions) 6 mercapto 1 hexanol, by Merck Group (1 mentions) Trifluoperazine, by Merck Group (1 mentions) Thioridazine, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Glacial acetic acid, by Thermo Fisher Scientific (1 mentions) Tocol, by Matreya (1 mentions) Ubiquinone 9, by Merck Group (1 mentions)

Spelling variants of this product

Ubiquinone (5 citations)

4 protocols using ubiquinone 10

Synthesis and Characterization of Antioxidant Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

CL from bovine heart and 1-palmitoyl-2-oleoyl-phosphatidylglycerol (POPG) were derived from Avanti Polar Lipids Inc. (Alabaster, AL, US) in the form of lyophilized powder. Yeast ubiquinone-6 was also from Avanti. Ubiquinone-10 and α-tocopherol were bought from Sigma-Aldrich (St. Louis, MO, US). Sodium borohydride and chloroform (99.8%) were purchased from Roth (Karlsruhe, Germany). Chemicals for buffer solutions were ordered from Sigma-Aldrich or Roth. Mini-Extruder and the porous membranes were supplied by Avanti. Azo initiator 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile), or MeO-AMVN, was delivered by Wako Pure Chemical Industries (Osaka, Japan). HPMC was a gift of Dr. Vitaly Roginsky. Diverse triphenylphosphonium-containing quinone-based antioxidants (see Figure 2) were synthesized in their oxidized (quinone) forms as previously described [63 (link)].

Lokhmatikov A.V., Voskoboynikova N., Cherepanov D.A., Skulachev M.V., Steinhoff H.J., Skulachev V.P, & Mulkidjanian A.Y. (2016). Impact of Antioxidants on Cardiolipin Oxidation in Liposomes: Why Mitochondrial Cardiolipin Serves as an Apoptotic Signal?. Oxidative Medicine and Cellular Longevity, 2016, 8679469.

+ Open protocol

+ Expand

Membrane Protein Reconstitution Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Escherichia coli polar phospholipid extract in chloroform (Avanti), menaquinone-7 (FUJIFILM Wako), Ni-NTA Agarose Resin (Thermo Fisher Scientific), 8%-16% Mini-PROTEAN TGX precast gels (Bio-Rad), and 2-heptyl-4-hydroxyquinoline-N-oxide (Santa Cruz Biotech) were purchased for use in this study. Chlorpromazine, trifluoperazine, thioridazine, 6-mercapto-1-hexanol (99%), ubiquinone-10 and 8-hydroxypyrene-1,3,6trisulfonic acid were purchased from Sigma-Aldrich. The styrene maleic anhydride polymer XIRAN SL30010 P20 was kindly provided by Polyscope.

Nakatani Y., Shimaki Y., Dutta D., Muench S.P., Ireton K., Cook G.M, & Jeuken L.J.C. (2020). Unprecedented Properties of Phenothiazines Unraveled by a NDH-2 Bioelectrochemical Assay Platform. Journal of the American Chemical Society, 142(3).

+ Open protocol

+ Expand

Synthesis and Characterization of Lipid Standards

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Butylated hydroxytoluene (BHT), bis-tris-propane (BTP), lithium acetate dehydrate, and ubiquinone-10 (UN10) were purchased from Sigma Aldrich (St. Louis, MO). Tocol was purchased from Matreya, Inc. (Pleasant Gap, Pa.). Ethanol (EtOH) (100%) was obtained from Pharmco (Brookfield, CT). Acetonitrile (MeCN), dichloromethane (DCM), glacial acetic acid (AA), hexane, and methanol (MeOH) were HPLC grade and purchased from Fisher (Pittsburgh, PA). Hexane and MeOH were kept refrigerated before and during extractions. Ultrapure deionized water (H₂O) was used throughout. Retinyl laurate, δ-tocopheryl laurate, n-butyl-β-apo-8′-carotenoate, and butylated UL10 (Bu-UL10) and UN10 (Bu-UN10) were synthesized as previously described [45 (link)–47 (link)].

Halm B.M., Lai J.F., Morrison C.M., Pagano I., Custer L.J., Cooney R.V, & Franke A.A. (2014). In vivo changes in plasma coenzyme Q10, carotenoid, tocopherol, and retinol levels in children after computer tomography. Archives of biochemistry and biophysics, 547, 37-43.

+ Open protocol

+ Expand

Purification of Quinone Standards for Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standards of menaquinone-8, demethylmenaquinone-8, and ubiquinone-8 were prepared from E. coli K-12 extracts and HPLC purified (Kim et al. 2008; (link)Latimer et al. 2021) . Plastoquinone-9 and plastochromanol-8 were HPLC purified from Arabidopsis leaf extracts (Block et al. 2013) . Demethylphylloquinone was extracted from the leaves of Arabidopsis DPhQ-MT knockout plants (GABI_565F06; Lohmann et al. 2006 (link)) and HPLC purified using the same method than that described for phylloquinone analysis in Widhalm et al. (2012) . Ubiquinone-9 and ubiquinone-10 were from Sigma-Aldrich. Phylloquinone was from MP Biomedicals. Quinol standards were prepared by reduction of their corresponding quinone forms with 20 mM NaBH 4 . Unless mentioned otherwise, other reagents were from Fisher Scientific.

Stutts L., Latimer S., Batyrshina Z., Dickinson G., Alborn H., Block A.K, & Basset G.J. (2023). The evolution of strictly monofunctional naphthoquinol C-methyltransferases is vital in cyanobacteria and plastids. The Plant cell, 35(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!