Abt 737

For ex vivo pituitary culture, anterior pituitaries were isolated from Hesx1^Cre/+;Ctnnb1^lox(ex3)/+ embryos at 18.5 dpc, placed on top of 0.2 µM Whatman filters (SLS) in 24 well plates containing 500 µl of media (DMEM-F12 (Gibco), 1% Pen/Strep (Sigma) and 1% FBS (PAA) supplemented with either ABT-737 at 2.5 µM (Adooq Bioscience), ABT-263 at 1 µM (Caymen Chemical) or vehicle. Anterior pituitaries were cultured for 3–4 days, changing media every day and subsequently processed for histological analysis.

Tetraethylorthosilicate, triethanolamine, cetyltrimethylammonium chloride solution (25 wt% in water), triethylamine (TEA), HCQ, Sepharose CL-4B matrix, and crystal violet reagent were purchased from Sigma-Aldrich. DSPC, DSPE-PEG₂₀₀₀, and Chol were purchased from Avanti Polar Lipids. Sucrose octasulfate (SOS) sodium salt was obtained from Toronto Research Chemicals, Inc., and converted to sucrose octasulfate triethylamine salt (TEA₈SOS) through an ion exchange chromatography process^{24 (link),26 (link)}. PAL was purchased from LC Laboratories. ABT-737 was purchased from AdooQ Bioscience. Matrigel matrix basement membrane was purchased from BD Bioscience. Premo™ autophagy tandem sensor RFP-GFP-LC3B Kit, Tali cell cycle kit, and CellROX™ Green flow cytometry reagents were purchased from Thermo Fisher Scientific. BrdU cell proliferation assay kit and senescence β-galactosidase staining kit were purchased from Cell Signaling Technology.