Anti e cadherin rabbit polyclonal antibody

Immunohistochemistry was performed using a power vision two-step kit (ZhongShan Golden Bridge Biotechnology, China). Paraffin-embedded fixed tissue sections (5 μm) were deparaffinized, rehydrated, and pretreated for antigen retrieval in Tris/EDTA. The sections were incubated with anti-E-cadherin rabbit polyclonal antibody and anti-α-smooth muscle actin (α-SMA) mouse monoclonal antibody (Santa Cruz Biotechnology, USA) at 4°C overnight, with the secondary antibody at room temperature for 30 min. Positive immunoreaction was identified after incubation with 3,3-diaminobenzidine tetrahydrochloride (ZhongShan Golden Bridge Biotechnology) and counterstaining with hematoxylin. Negative controls without primary antibodies were carefully examined for each reaction. Sections were photographed with an upright fluorescence microscopic imaging system (BX51 Olympus, Japan). To analyze the expression of E-cadherin and α-SMA in glomeruli and interstitium, 10 nonoverlapping high-power fields (magnification 400×) were randomly selected from each kidney section. Data are reported as the mean optical density in each field area.