Body surface potential differences were recorded as the voltage between two silver wires placed subcutaneously at the right shoulder and left hip using an EXT-02 B amplifier (npi electronic). Data were digitized at 20 kHz (custom-written LabVIEW routines controlling PXIe-6363, National Instruments) for post hoc analysis. Muscle activity was extracted by applying a fast Fourier transform to the data and determining the power in the range 200 Hz—1 kHz. Fold increase was determined as power / powerBaseline.
Pxie 6363
Manufactured by National Instruments
Sourced in United States
The PXIe-6363 is a high-performance data acquisition (DAQ) module designed for the PXI Express (PXIe) platform. It features 32 analog input channels, 4 analog output channels, and 48 digital input/output channels. The module is capable of sampling rates up to 2 MS/s per channel and supports a variety of measurement and control applications.
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6 protocols using pxie 6363
1
Quantifying Muscle Activity using EXT-02 B
2
PXIe-Based Hardware Interface for Experiments
3
Head-fixed Treadmill Locomotion Tracking
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Head immobilized mice were placed on a custom designed linear treadmill. The treadmill was either freely movable so that animals could move at will, or under motor control. The motion of the belt of the treadmill was monitored with a mechanically coupled optical encoder. The signal of the optical encoder was digitized at 20 kHz simultaneously with the position signal of the slow scan mirror (custom-written LabVIEW routines controlling PXIe-6363, National Instruments) for post hoc determination of movement velocity during corresponding images.
4
Neurophysiological Signal Acquisition and Spike Sorting
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Wide band (0.1–8000 Hz) neurophysiological signals from silicon probes (Neuronexus, Buzsaki-64) were amplified 1000 times via Intan RHD2000-series Amplifier evaluation system or Plexon VLSI headstages and a PBX2 amplifier and continuously acquired at 20 kHz on two synchronized National Instruments A/D cards (PXi-e 6363, 16-bit resolution). Spike sorting was performed semi-automatically using the clustering software KlustaKwik (http://klustakwik.sourceforge.net ) and the graphical spike sorting application Klusters (http://klusters.sourceforge.net )62 (link).
5
Custom Pinch Force Measurement Apparatus
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A custom pinch apparatus (Figure 2 ) was constructed utilizing two 6-DOF load cells (Mini40, ATI Industrial Automation, Apex, NC, United States). The designated locations for applying pinch force included a surface for the index finger in parallel to a surface for the thumb. Both locations could accommodate surfaces as either a metal bar (rigid surface) or an elastic band (compliant surface). The band was set to provide approximately constant compliance of 1.5 N/cm normal to the surface. Data was acquired on a multi-input/output data acquisition system (PXIe-6363 with BNC interface, National Instruments, Austin, TX, United States). Force data was sampled at 100 Hz and processed in software developed in Simulink (Mathworks, Natick, MA, United States). The force trace was displayed in real-time on a 27-inch monitor (Dell P2717H).
6
Acoustic Occlusion Characterization
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The experimental setup is presented in Fig. 2 a. The occluder was realized by a pair of acoustic drywall plates with two layers of Suprema—Tecsound pallet sandwiched between them. This 3 cm thick occluder was placed perpendicularly to the wall at a distance of 45 cm. Noise was generated by playing two different Gaussian random white noises through two audio speakers (MIYAKO Ltd, SL-800). The microphone array consisted of 16 condenser microphones (BOYA, BY-M1) placed at a spacing of 4 cm, and were sampled simultaneously at 40 kHz with 16-bit depth using a multichannel DAQ device (National Instruments, PXIe-6363). The array was placed at a distance of 53 cm from the wall, in parallel to it, and the rightmost microphone was at a distance of 5 cm from the occluder. A human subject served as the target in all experiments. The figures were created using MATLAB V. R2022a (https://www.mathworks.com/) and INKSCAPE V. 1.2 (https://inkscape.org/).
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