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Anti mouse igg3 antibody

Manufactured by Abcam
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Anti-mouse IgG3 antibody is a primary antibody that specifically binds to the IgG3 subclass of immunoglobulins in mice. It can be used for the detection, quantification, and purification of mouse IgG3 in various immunoassays and research applications.

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Lab products found in correlation

Z0622, by Agilent Technologies (2 mentions) Goat anti rabbit alexa 546, by Thermo Fisher Scientific (2 mentions) Anti rabbit envision k4009, by Agilent Technologies (2 mentions) Cy5 tyramide, by PerkinElmer (2 mentions) Fluorescein tyramide, by PerkinElmer (2 mentions)

2 protocols using anti mouse igg3 antibody

1

Multiplexed Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The multiplexing protocol has been presented before54 (link). Briefly, tissue sections were subjected to the same deparaffinization, antigen retrieval and blocking protocol mentioned above. Staining for pan-cytokeratin, Siglec-15 and CD68 was performed using a sequential multiplexed immunofluorescence protocol with isotype-specific primary antibodies to detect epithelial tumor cells (cytokeratin: clone Z0622, Agilent), Siglec-15 and CD68 (PG-M1, mouse IgG3, Agilent). Nuclei were highlighted using DAPI. Secondary antibodies and fluorescent reagents used were goat anti-rabbit Alexa546 (Molecular Probes) for cytokeratin detection, anti-rabbit Envision (K4009, Agilent) with Cy5-tyramide (Perkin Elmer) for Siglec-15 detection and anti-mouse IgG3 antibody (1:1000, Abcam, Cambridge, MA) with fluorescein-tyramide (Perkin Elmer) for CD68 detection. Residual horseradish peroxidase activity between incubations with secondary antibodies was eliminated by exposing the slides twice for seven minutes to a solution containing benzoic hydrazide and hydrogen peroxide.
Wang J., Sun J., Liu L.N., Flies D.B., Nie X., Toki M., Zhang J., Song C., Zarr M., Zhou X., Han X., Archer K.A., O’Neill T., Herbst R.S., Boto A.N., Sanmamed M.F., Langermann S., Rimm D.L, & Chen L. (2019). Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nature medicine, 25(4), 656-666.
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2

Multiplexed Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The multiplexing protocol has been presented before54 (link). Briefly, tissue sections were subjected to the same deparaffinization, antigen retrieval and blocking protocol mentioned above. Staining for pan-cytokeratin, Siglec-15 and CD68 was performed using a sequential multiplexed immunofluorescence protocol with isotype-specific primary antibodies to detect epithelial tumor cells (cytokeratin: clone Z0622, Agilent), Siglec-15 and CD68 (PG-M1, mouse IgG3, Agilent). Nuclei were highlighted using DAPI. Secondary antibodies and fluorescent reagents used were goat anti-rabbit Alexa546 (Molecular Probes) for cytokeratin detection, anti-rabbit Envision (K4009, Agilent) with Cy5-tyramide (Perkin Elmer) for Siglec-15 detection and anti-mouse IgG3 antibody (1:1000, Abcam, Cambridge, MA) with fluorescein-tyramide (Perkin Elmer) for CD68 detection. Residual horseradish peroxidase activity between incubations with secondary antibodies was eliminated by exposing the slides twice for seven minutes to a solution containing benzoic hydrazide and hydrogen peroxide.
Wang J., Sun J., Liu L.N., Flies D.B., Nie X., Toki M., Zhang J., Song C., Zarr M., Zhou X., Han X., Archer K.A., O’Neill T., Herbst R.S., Boto A.N., Sanmamed M.F., Langermann S., Rimm D.L, & Chen L. (2019). Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nature medicine, 25(4), 656-666.
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