Log In Sign up
The largest database of trusted experimental protocols

7 aad viability staining solution

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

7-AAD Viability Staining Solution is a fluorescent dye used for cell viability assessment. It intercalates with DNA and emits fluorescence upon binding, allowing the identification of non-viable cells.

Automatically generated - may contain errors

Lab products found in correlation

Facsdiva software, by BD (3 mentions) Celltrace violet, by Thermo Fisher Scientific (3 mentions) Facscalibur, by BD (2 mentions) Streptavidin percp, by BD (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) 40 μm cell strainer, by BD (2 mentions) Facsaria 2, by BD (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Live dead fixable dead cell stain, by Thermo Fisher Scientific (1 mentions) Mouse gm csf, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Streptavidin microbeads, by Miltenyi Biotec (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd16 32, by Thermo Fisher Scientific (1 mentions) Lsrii fortessa flow cytometer, by BD (1 mentions) Anti human cd3 apc, by BD (1 mentions) Fc blocker, by BioLegend (1 mentions) Facscelesta, by BD (1 mentions) Lsrfortessa sorp, by BD (1 mentions)

Close spelling variants of this product

H7411)7-AAD Viability Staining Solution EBioscience™ 7-AAD Viability Staining Solution 7-Aminoactinomycin D (7-AAD) Viability Staining Solution 7-AAD viability staining 7-AAD Viability Solution 7-AAD staining solution 7-AAD staining 7-AAD solution 7-AAD

41 protocols using 7 aad viability staining solution

1

Cell Viability Quantification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were seeded in a 24-well plate (150,000 cells/well, 0.5 mL/well RPMI 10%FBS) and treated with DMSO or RGFP966. After 48 h, each condition was harvested and resuspended in 190 µL PBS to which 5 µl Counting Beads (Spherotech AccuCount Blank Particles ACBP-70-10) and 5 µl 7-AAD (eBioscience™ 7-AAD Viability Staining Solution) were added. Measurement was acquired on BD LSRFortessa™. Data was analyzed with FlowJo software (BD). Live-cell (7-AAD) count was normalized to bead count.
Kugler E., Madiwale S., Yong D., Thoms J.A., Birger Y., Sykes D.B., Schmoellerl J., Drakul A., Priebe V., Yassin M., Aqaqe N., Rein A., Fishman H., Geron I., Chen C.W., Raught B., Liu Q., Ogana H., Liedke E., Bourquin J.P., Zuber J., Milyavsky M., Pimanda J., Privé G.G, & Izraeli S. (2023). The NCOR-HDAC3 co-repressive complex modulates the leukemogenic potential of the transcription factor ERG. Nature Communications, 14, 5871.
+ Open protocol
+ Expand
2

Peritoneal Macrophage Dynamics in Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry analysis was performed on samples retrieved by peritoneal lavage at 2, 4, and 7 d following implantation as described previously [26 (link)]. Briefly, 5 × 105 cells were resuspended in FACS buffer (1% BSA in PBS, 0.01% NaN3) and placed in CD16/CD32 (Fc block; Becton Dickinson) for 20 min at 4°C. Cells were fixed in 1% formaldehyde and then stained with anti-mouse F4/80 FITC Ab or an isotype control Ab to evaluate macrophage accumulation into the peritoneal cavity. For in vivo experiments, five mice per time point per genotype were used and the experiment was performed in triplicate. Dead/apoptotic cells were detected by 7AAD viability staining solution (eBioscience) according to supplier’s instructions. Peritoneal macrophages isolated on d2 post-implantation (5×105cells per sample), were also stained for CD-36 (CD36-PE (eBioscience)) following exposure to 10 ng/ml IL-4. Flow cytometry samples were acquired on an LSRII (BD Biosciences) and analysis was performed with FlowJo software (Tree Star, Ashland, OR, USA).
Moore L.B., Sawyer A.J., Charokopos A., Skokos E.A, & Kyriakides T.R. (2014). Loss of MCP-1 alters macrophage polarization and reduces NFκB activation in the foreign body response. Acta biomaterialia, 11, 37-47.
+ Open protocol
+ Expand
3

OVA and MCCp Peptide Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols
OVAp-II (OVA 323–339; ISQAVHAAHAEINEAGR) and OVAp-I (OVA 257–264; SIINFEKL) were generated at the Centro de Biología Molecular Severo Ochoa (CBM, Madrid) and Centro Nacional de Biotecnología (CNB-CSIC, Madrid). Moth cytochrome c (MCCp) 88–103 peptide (ANERADLIAYLKQATK) was purchased from GenScript. Other reagents used were mouse GM-CSF (Peprotech), LPS (Sigma-Aldrich), streptavidin microbeads (Miltenyi Biotec), streptavidin-PercP (Becton Dickinson), poly-L-lysine (Sigma-Aldrich), CellTrace Violet, Alexa Fluor568-phalloidin (both from Life Technologies), 7-AAD Viability Staining Solution (eBiosciences), and Live/Dead Fixable dead cell stain (Thermo Fisher). Percp-Streptavidin (1:300 dilution, BD Biosciences), Allophycocyanin-labelled dextramers specific for OVA H-2Kb (257-SIINFEKL-264) were purchased from Immudex.
Cruz-Adalia A., Ramirez-Santiago G., Osuna-Pérez J., Torres-Torresano M., Zorita V., Martínez-Riaño A., Boccasavia V., Borroto A., Martínez del Hoyo G., González-Granado J.M., Alarcón B., Sánchez-Madrid F, & Veiga E. (2017). Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses. Nature Communications, 8, 1591.
+ Open protocol
+ Expand
4

Immunophenotyping of PD-L1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
After treatment with atezolizumab, cells from treated and non-treated wells were trypsinized, washed, and re-suspended in 100 µL staining buffer (phosphate-buffered saline (PBS) with 2% FCS and 0.1% sodium azide) for surface staining. To gate out dead cells, 7AAD viability staining solution (eBioscience, San Diego, CA, USA) was used. PD-L1-Allophycocyanin (APC) (clone MIH1, eBioscience, San Diego, CA, USA) was then added and cells kept in 4 °C for 30 min. Cells were then washed twice with staining buffer and re-suspended in 300 µl for analyses. Data were acquired on BD LSRFortessa flow cytometer using BD FACSDiva software (BD Biosciences, San Jose, CA, USA) and analyzed on FlowJo version 10 software (BD Biosciences, San Jose, CA, USA).
Ali M.H., Toor S.M., Rakib F., Mall R., Ullah E., Mroue K., Kolatkar P.R., Al-Saad K, & Elkord E. (2019). Investigation of the Effect of PD-L1 Blockade on Triple Negative Breast Cancer Cells Using Fourier Transform Infrared Spectroscopy. Vaccines, 7(3), 109.
+ Open protocol
+ Expand
5

Quantifying Macrophage SRA Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression level of SRA on macrophages in the lung, liver, and brain was assayed with an LSRII/Fortessa flow cytometer (BD Biosciences, Heidelberg, Germany). Briefly, MNCs were prepared as previously described (27 (link)). First, cells were blocked by CD16/32 (14-0161-82; Invitrogen, Waltham, MA, USA) and then stained with 7-AAD viability staining solution (eBioscience, San Diego, CA), Pacific Blue anti-mouse/human CD11b Ab (eBioscience, San Diego, CA), PE-conjugated anti-F4/80 Ab (eBioscience, San Diego, CA), anti-mouse SRA antibody (sc-166184; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and the secondary antibody goat anti-mouse IgG H&L (Alexa Fluor 488) (ab150113). Flow cytometric data were then analyzed using FlowJo software (Tree Star, Ashland, OR, USA).
Li L., Luo J., Zhu Z., Xu Q., Wang P., Chang B., Wang D., Yu L., Lu X., Zhou J., Zuo D, & Chen Q. (2022). SRA Suppresses Antiviral Innate Immune Response in Macrophages by Limiting TBK1 K63 Ubiquitination via Deubiquitinase USP15. Microbiology Spectrum, 10(6), e02028-22.
+ Open protocol
+ Expand
6

T-cell Immunophenotyping of MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human and porcine MSCs were incubated with phosphate‐buffered saline containing 1% bovine serum albumin or porcine serum, respectively. Additionally, Fc blocker (BioLegend, San Diego, CA) was added to cells for 10 minutes at a concentration of 1 × 106 cell per milliliter in order to reduce nonspecific binding. Anti‐human CD3 APC (BD, Biosciences, San Jose, CA) and anti‐porcine CD3e AF647 (BD, Biosciences) were used for assessing pan T cells. Cells were stained with preconjugated antibodies according to manufacturer's instruction. Following antibody binding, 7AAD viability staining solution (eBioscience, Grand Island, NY) was added for an additional 5 minutes to exclude dead cells. Acquisition was carried out on a BD FACSCelesta using the BDFACS Diva software (BD, Biosciences). Analysis was completed using FlowJo analysis software.
Xu A.L., Rodriguez LA I.I., Walker KP I.I.I., Mohammadipoor A., Kamucheka R.M., Cancio L.C., Batchinsky A.I, & Antebi B. (2019). Mesenchymal Stem Cells Reconditioned in Their Own Serum Exhibit Augmented Therapeutic Properties in the Setting of Acute Respiratory Distress Syndrome. Stem Cells Translational Medicine, 8(10), 1092-1106.
+ Open protocol
+ Expand
7

Quantifying Cell Proliferation Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were transfected with siRNAs against the proteins of interest or non-targeting siRNAs. 72 h post‐transfection cells were incubated with 10 μM EdU for 1 h before collection and fixed in 4% paraformaldehyde solution. Permeabilization of the cells was performed by incubation in 1% BSA in PBS. Click reaction was performed (Alexa Fluor 647 (Thermo Fisher Scientific), 10 mM (+)‐sodium l‐ascorbate and 2 mM copper (II) sulfate pentahydrate in PBS) for 1 h. Subsequently, cells were incubated for 20 min with 7‐AAD viability staining solution (eBioscience) before analysis by flow cytometry using a BD LSRFortessa SORP.
Mosler T., Baymaz H.I., Gräf J.F., Mikicic I., Blattner G., Bartlett E., Ostermaier M., Piccinno R., Yang J., Voigt A., Gatti M., Pellegrino S., Altmeyer M., Luck K., Ahel I., Roukos V, & Beli P. (2022). PARP1 proximity proteomics reveals interaction partners at stressed replication forks. Nucleic Acids Research, 50(20), 11600-11618.
+ Open protocol
+ Expand
8

Multiparameter Analysis of Mouse Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens and bone marrow were harvested. Single cell suspensions were prepared in PBS supplemented with 2% FBS and 2mM EDTA and filtered through a 40μm cell strainer (BD Biosciences, Mississauga, ON, Canada) to remove cell aggregates. Single cell suspensions were counted manually and viability determined by trypan blue exclusion was always >95%. Cells (1x106) were then incubated with 0.5 μg of anti-CD16/CD32 monoclonal antibodies (eBioscience Inc, San Diego, CA, USA) prior to staining with the following fluorescence-conjugated antibodies purchased from eBioscience: anti-mouse TER119-PE (clone TER119), anti-mouse CD71-FITC (clone R17217) and anti-mouse CD44-APC (clone IM7). Non-viable cells were excluded using 7-AAD viability staining solution (eBioscience Inc, San Diego, CA, USA). Cells were acquired using BD FACSCanto II (BD Biosciences, Mississauga, ON, Canada) and data were analysed using FlowJo version 9.3.3.
Laroque A., Min-Oo G., Tam M., Ponka P., Stevenson M.M, & Gros P. (2017). The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria. PLoS ONE, 12(5), e0177818.
+ Open protocol
+ Expand
9

Apoptosis Assessment by Annexin V-7-AAD

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Many agents, including the clinically useful sorafenib, can induce apoptosis [20 (link)]. Following 24-h treatment with 8 μM sorafenib, PANC-1–derived clones were trypsinized, washed with cold PBS, and resuspended in PBS. The advantage of 7-amino-actinomycin D (7-AAD) over propidium iodide is that there is minimal spectral overlap between the emissions. We used a fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit with 7-AAD (#640922; BioLegend, USA) according to the manufacturer’s instructions. Briefly, 5 μL FITC–annexin V and 5 μL 7-AAD Viability Staining Solution (#00-6993; eBioscience, USA) were added to a 100-μL cell suspension (1 × 106 cells) in binding buffer. The cells were gently vortexed and incubated for 15 min at room temperature in the dark, and 400 μL binding buffer was added for flow cytometric analysis using a FACScan Flow Cytometer (Becton Dickinson).
Zhu Y., Zhang J.J., Xie K.L., Tang J., Liang W.B., Zhu R., Zhu Y., Wang B., Tao J.Q., Zhi X.F., Li Z., Gao W.T., Jiang K.R., Miao Y, & Xu Z.K. (2014). Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling. Journal of Translational Medicine, 12, 309.
+ Open protocol
+ Expand
10

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from the BM and peripheral blood were first incubated with human BD Fc block (BD Biosciences) to block Fcγ receptor and then stained with the following anti-human Abs: CD19-APC (clone HIB19, BioLegend), CD34-PE (clone 563, BD Biosciences), CD20-FITC (clone 2H7, BioLegend), CD27-APC/Cyanine7 (clone O323, BioLegend), IgD-PE (clone IA6-2, BD Biosciences), IgM-FITC (clone G20-127, BD Biosciences), CD43-PE (clone CD43-10G7, BioLegend), CD38-PE/Cy7 (clone HIT2, BioLegend), CD38-Percp/Cy5.5 (clone HIT2, BD Biosciences), CD24-PE (clone ML5, BD Biosciences), CD27-V450 (clone M-T271, BD Biosciences), IgD-BV510 (clone IA6-2, BD Biosciences), IgG-PE/Cy7 (clone G18-145, BD Biosciences), Ki-67–PE (clone Ki-67, BioLegend), CD80-FITC (clone L307.4, BD Biosciences), CD86-PE (clone IT2.2, BD Biosciences), HLADR-PE (clone G46-6, BD Biosciences) and CD69-PE/Cy7 (clone FN50, BD Biosciences). 7-AAD Viability Staining Solution (eBioscience, Thermo Fisher Scientific) was used for live versus dead cell discrimination. Samples were analyzed with a FACSVerse flow cytometer (BD Biosciences) using the FACSuite software. Data analysis was performed with FlowJo 10 software (Treestar).
Min Q., Meng X., Zhou Q., Wang Y., Li Y., Lai N., Xiong E., Wang W., Yasuda S., Yu M., Zhang H., Sun J., Wang X, & Wang J.Y. (2021). RAG1 splicing mutation causes enhanced B cell differentiation and autoantibody production. JCI Insight, 6(19), e148887.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!