Hematoxylin and eosin (H&E) breast tissue slides were retrieved for biopsy-confirmed BBD patients who gave permission to review their biopsy records (18 (link),20 (link),21 (link)). H&E slides were available for 488 cases and 2124 controls (i.e., full nested case-control study group) for centralized pathology review (5 (link),18 (link),19 (link)). Within this group, a total of 3836 slides were digitized into WSIs at 20× (n=234) or 40× (n=3602) magnification using the Panoramic SCAN 150 (3DHISTECH Ltd, Budapest, Hungary). For women with good quality slides, up to six slides from different tissue blocks were digitized. H&E slides that could not be digitized were due to poor quality, slides too thick to fit into scanner, and plastic mounting covers. Attempts to create new H&E slides were not always possible due to missing (or returned to hospital) blocks, old-style blocks not created using tissue cassettes, or poor-quality blocks.
Panoramic scan 150
Manufactured by 3DHISTECH
Sourced in Hungary
The Panoramic SCAN 150 is a high-performance whole slide scanning system designed for fast and efficient digitization of histological samples. The core function of this equipment is to capture high-quality digital images of entire microscope slides at a resolution up to 0.25 μm/pixel. The system is capable of scanning samples with a maximum size of 150 mm x 75 mm.
Automatically generated - may contain errors
Lab products found in correlation
Casecenter 2.9 viewer, by 3DHISTECH (2 mentions)
Cm1950 cryomicrotome, by Leica (2 mentions)
Super resolution phosphor screen, by PerkinElmer (2 mentions)
Cyclone plus phosphor imager, by PerkinElmer (2 mentions)
4 6 diamidine 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 igg, by Thermo Fisher Scientific (1 mentions)
Fluoromount g, by Clinisciences (1 mentions)
Nbp1 31231, by Novus Biologicals (1 mentions)
Rm2255, by Leica (1 mentions)
Rm2245, by Leica (1 mentions)
7 protocols using panoramic scan 150
1
Digitalization of Breast Biopsy Slides for Pathology Review
2
Digitizing Whole Slide Images from H&E Slides
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H&E slides were digitized into whole slide images at 20× or 40× using the Panoramic SCAN 150 (3DHISTECH Ltd, Budapest, Hungary). For women with good-quality slides, up to six slides from different tissue blocks were digitized. H&E slides that were not digitized were due to poor quality, slides too thick to fit into scanner, and plastic mounting coverslips. Attempts to create new H&E slides were not always possible due to missing (or returned to hospital) blocks, old-style blocks not created using tissue cassettes, or poor-quality blocks [22 (link)]. Out of all controls in the original nested case–control study (n = 1920), 1083 (or 56%) had their slides successfully digitized into whole slide images (WSI) (Fig. 1 ). Women with and without available tissue readings had similar distributions of breast cancer risk factors [23 (link)].
3
Digitizing H&E Slides for Cancer Study
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H&E slides were digitized into whole-slide images at × 20 (n = 93) or × 40 (n = 890) using the Panoramic SCAN 150 (3DHISTECH Ltd., Budapest, Hungary). For women with good-quality slides, up to six slides from different tissue blocks were digitized. H&E slides that were not digitized were due to poor quality, slides too thick to fit into the scanner, and plastic mounting coverslips. Attempts to create new H&E slides were not always possible due to missing (or returned to hospital) blocks, old style blocks not created using tissue cassettes, or poor-quality blocks [29 (link)]. Slides were successfully digitized for approximately 80% of all control women in the original nested case-control study.
4
Quantification of Colonic Muc2 Protein
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Mucin 2 (Muc2) proteins were labelled on formalin-fixed samples that were cut, using a microtome (Leica RM2255, Leica, Wetzlar, Germany), into 5-μm-thick sections and mounted on adhesive microscope slides (Adhesives slides KP printer, KliniPath, Duiven, The Netherlands). Immunostaining was performed using the Leica Bond RXm. Heat-induced antigen retrieval was done with Leica Bond™ Epitope Retrieval 1 (pH6) for 20 min. Rabbit polyclonal antibody against the MUC2 protein (NBP1-31231, Novus Biologicals, Littleton, CO, USA), diluted at 1/500, was revealed by a goat anti-rabbit Alexafluor 568 IgG (dilution 1/2000, Invitrogen, Waltham, MA, USA) with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, Invitrogen, Waltham, MA, USA) to counterstained nuclear. All the reagents were diluted with Bond Antibody Diluent (AR9352, Leica, Wetzlar, Germany). Slides were mounted using a fluorescent mounting medium (Fluoromount-G, Clinisciences, Nanterre, France), scanned with the Panoramic Scan 150 (3D Histech, Budapest, Hungary) and analyzed with the CaseCenter 2.9 viewer (3D Histech, Budapest, Hungary). Ten crypt (colon) or villus/crypt (ileum) units were analyzed in each sample, and the number of Muc2 cells was counted according to Fukuda et al. [17 (link)]. Tissue samples with non-well-oriented crypts were removed from the analysis.
5
Zebrafish Gut Histomorphometry
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Histological sections were used to compare microscopical tissular organization between Conv, GF and mix9 zebrafish larvae. A total of 5 fish were sacrificed and fixed for 1 day in Carnoy’s fixative. Whole fixed animals were then dehydrated in methanol (2 × 30 minutes) then in ethanol 100 % (2 × 20 min). Final dehydration was performed by 100 % xylene solution 2 × 2 hours. Then, samples were embedded in paraffin wax solution (3 × 2 hours) and embedded in paraffin wax for polymerization. Sections (thickness 5μm) were cut with a microtome RM2245 (Leica Microsystems GmbH, Wien, Austria), and mounted on adhesive slides (Klinipath- KP-PRINTER ADHESIVES). Paraffin-embedded sections were deparaffinized and stained with Alcian Blue (AB) and Periodic-Acid Schiff (PAS) to observe both neutral and acidic mucins and Goblet cells quantification. All slides were scanned with the Panoramic Scan 150 (3D Histech) and analyzed with the CaseCenter 2.9 viewer (3D Histech). Goblet cells quantification was estimated by manual counting of total AB positive cells in blue per villi of the posterior gut.
6
Ins-1E Tumor Imaging with GLP-1 Analogs
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Ins-1E-bearing mice were administered 100 pmol (0.4-0.9 MBq) of [Nle 14 ,Lys 40 (Ahx-DOTA-68 Ga)NH 2 ]Ex-4 or [Lys 40 (NODAGA-68 Ga)NH 2 ]Ex (9-39) in 100 mL of sterile saline via intravenous injection. One hour after injection, mice were euthanized, and 20-to 40-min static scans were acquired using a microPET Focus 120 scanner (Concorde Microsystems), followed by 2-min CT scans on a micro-CT-Tomoscope Synergy system (CT Imaging GmbH).
Inhibitory Concentration of 50% (IC 50 ) Determination and Autoradiography IC 50 values were measured using in vitro receptor autoradiography on frozen sections of human insulinomas and frozen Ins-1E cell pellets with 125 I-GLP-1(7-36)NH 2 as a radioligand, essentially as described previously (1) . For ex vivo digital autoradiography, tumors were fast-frozen on dry ice and embedded in optimum-cutting-temperature compound, and then 10-mm sections were cut using a Leica CM1950 cryomicrotome. Sections were exposed on a Super Resolution phosphor screen (Perkin Elmer) for 7 d. The digital autoradiography images were obtained by scanning the phosphor screens on the Cyclone Plus Phosphor Imager (Perkin Elmer). Adjacent 10-mm slices were stained with hematoxylin and eosin and scanned using Panoramic SCAN 150 (3D Histech).
Inhibitory Concentration of 50% (IC 50 ) Determination and Autoradiography IC 50 values were measured using in vitro receptor autoradiography on frozen sections of human insulinomas and frozen Ins-1E cell pellets with 125 I-GLP-1(7-36)NH 2 as a radioligand, essentially as described previously (1) . For ex vivo digital autoradiography, tumors were fast-frozen on dry ice and embedded in optimum-cutting-temperature compound, and then 10-mm sections were cut using a Leica CM1950 cryomicrotome. Sections were exposed on a Super Resolution phosphor screen (Perkin Elmer) for 7 d. The digital autoradiography images were obtained by scanning the phosphor screens on the Cyclone Plus Phosphor Imager (Perkin Elmer). Adjacent 10-mm slices were stained with hematoxylin and eosin and scanned using Panoramic SCAN 150 (3D Histech).
7
Small-Animal PET/CT Imaging of Tumors
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Tumors were fast-frozen on dry ice, embedded in optimal-cuttingtemperature compound, and cut into 10-mm sections using a CM1950 cryomicrotome (Leica). Sections were exposed on a Super Resolution phosphor screen (Perkin Elmer) for 14 h. The digital autoradiography images were obtained by scanning the phosphor screens on a Cyclone Plus phosphor imager (Perkin Elmer). Adjacent 10-mm slices were stained with hematoxylin-eosin and scanned using Panoramic SCAN 150 (3D Histech).
Small-Animal PET/CT Imaging PET imaging was conducted on a microPET Focus 120 scanner (Concorde Microsystems) (22) . The mice were administered 89 Zr-DFO-AC-10 (6.9-7.5 MBq, 80-82 mg of mAb in 100 mL of sterile saline) via tail-vein injection. Full details are presented in the supplemental materials.
Small-Animal PET/CT Imaging PET imaging was conducted on a microPET Focus 120 scanner (Concorde Microsystems) (22) . The mice were administered 89 Zr-DFO-AC-10 (6.9-7.5 MBq, 80-82 mg of mAb in 100 mL of sterile saline) via tail-vein injection. Full details are presented in the supplemental materials.
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