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Lovastatin

Manufactured by Abcam
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Lovastatin is a lab equipment product that functions as a cholesterol-lowering compound. It is a naturally occurring statin derived from the fungus Aspergillus terreus.

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Lab products found in correlation

Rosuvastatin, by Abcam (2 mentions) Mevastatin, by Abcam (2 mentions) Simvastatin, by Abcam (2 mentions) Gradient gel, by Bio-Rad (1 mentions) Multiskan ascent plate reader, by Thermo Fisher Scientific (1 mentions) Celltiter 96 aqueous one solution mts, by Promega (1 mentions) Stempro osteogenesis differentiation kit, by Thermo Fisher Scientific (1 mentions) Ckx41, by Olympus (1 mentions) Timp3, by Abcam (1 mentions) Zoledronic acid, by Merck Group (1 mentions) Dasatinib, by Merck Group (1 mentions) Tipifarnib, by Merck Group (1 mentions) Mevalonic acid, by Merck Group (1 mentions) Mf 438, by Merck Group (1 mentions)

8 protocols using lovastatin

1

Lovastatin's Effects on Osteogenic Differentiation

Cited 2 times
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Fig. 1 demonstrates the overview of the present study design. Cells were plated onto silicon elastomer-based concave microwells (StemFIT 3D; MicroFIT) of 600 µm diameter at a density of 4×105 cells/well and cultured in osteogenic media (StemPro® Osteogenesis Differentiation Kit; Gibco; Thermo Fisher Scientific, Inc.) at 37°C. The medium was refreshed at 3-day intervals. To examine the effect of lovastatin, the cells were cultured in the presence of lovastatin (Abcam) at final concentrations of 0 (untreated control), 2 and 6 µM using dimethyl sulfoxide (DMSO) as the vehicle at plating. The concentrations of lovastatin used in the present study were based on those used in previously published studies (11 (link)–14 (link)). Equal amounts of DMSO were added to each culture sample to offset the influence of this dissolving vehicle (15 (link)). The cells expressed CD44 surface marker and the cell spheroids were positive for SSEA-4 (7 (link),16 (link)). The morphology of the microspheres was viewed under an inverted microscope (CKX41; Olympus Corporation) on days 2, 7 and 14 following plating. The diameter of the cell spheroids was measured at each time point.
Kim B.B., Tae J.Y., Ko Y, & Park J.B. (2019). Lovastatin increases the proliferation and osteoblastic differentiation of human gingiva-derived stem cells in three-dimensional cultures. Experimental and Therapeutic Medicine, 18(5), 3425-3430.
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2

Analyzing LRRK2 Activation in HEK293T Cells

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HEK293T cells were transfected with indicated plasmids; 24 h after transfection, cells were chilled, washed, and resuspended in PBS. Where applicable, cells were treated with 10 µM lovastatin (Abcam) at the time of transfection. Cell pellets were resuspended in lysis buffer (50 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 0.5 mM DTT, and 100 nM GTP, protease inhibitor cocktail, 0.1 µg/ml microcystin [Calbiochem], 1.15 mM NaF, and 5 mM sodium pyrophosphate). Lysates were incubated at 4°C with rotation for 15 min and spun at 16,873 relative centrifugal force in a microcentrifuge for 15 min. Lysate (120 µg) was analyzed by immunoblot after resolution on a 4–20% gradient gel (Bio-Rad). Relative activation of LRRK2 was determined using the ratio of pS1292/total LRRK2, normalizing for Rab29 levels.
Gomez R.C., Wawro P., Lis P., Alessi D.R, & Pfeffer S.R. (2019). Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases. The Journal of Cell Biology, 218(12), 4157-4170.
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3

ADC and PBD Cytotoxicity Assay

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10,000 cells per well were seeded in a flat bottom 96-well plate, and NCI-N87 cell were incubated overnight before treatment. For ADC growth inhibition assays, cells were then incubated with serial dilutions of ADCT-301 or ADCT-502 in triplicate. For the SG3199 growth inhibition assays, cells were mixed with a serial dilution of SG3199 in DMSO before seeding. Growth inhibition was measured after 96 hours in Karpas-299 cells and 144 hours in NCI-N87 cells using the CellTiter 96 AQueous One MTS Solution (Promega), and absorbance measured on a Multiskan Ascent plate reader (ThermoFisher) at 492 nm. Growth inhibition was calculated as a percentage of absorbance compared to an untreated control, and IC50 values were calculated using the sigmoidal, 4PL, X is log(concentration) equation in GraphPad Prism. For drug transporter inhibitor combination cytotox, the resistant cell lines were incubated overnight with either 5 μM MK-571 (ABCC2 inhibitor, Abcam), 10 μM FTC (ABCG2 inhibitor, Sigma), 5 μM Reversin-121 (ABCB1 inhibitor, Cayman Chemical) or 10 nM Lovastatin (SLCO2B1 inhibitor, Abcam) overnight before carrying out the ADC or PBD growth inhibition assay as previously described.
Corbett S., Huang S., Zammarchi F., Howard P.W., van Berkel P.H, & Hartley J.A. (2020). The role of specific ATP-binding cassette transporters in the acquired resistance to pyrrolobenzodiazepine dimer-containing antibody-drug conjugates. Molecular cancer therapeutics, 19(9), 1856-1865.
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4

Inhibition of TACE, TIMP3, and Caveolae

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TACE inhibitor, metalloproteinase inhibitor 3 (Timp3), and caveolae stability inhibitor, lovastatin, were purchased from Abcam (Eugene, OR, USA) and Sigma-Aldrich, respectively.
Park P.J, & Kim S.T. (2020). Caveolae-Associated Protein 3 (Cavin-3) Influences Adipogenesis via TACE-Mediated Pref-1 Shedding. International Journal of Molecular Sciences, 21(14), 5000.
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5

In vitro Compound Solubilization Protocol

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Compounds used for in vitro experiments were commercially available and listed below. Fluvastatin (abcam, ab120651), cerivastatin (abcam, ab142853), trametinib (biomol, GSK-1120212), mevalonic acid (sigma-aldrich, 50838), lovastatin (abcam, ab120614), dasatinib (Sigma-Aldrich, SML2589), tipifarnib (Sigma-Aldrich, SML1668) were solved in DMSO (AppliChem, A3672). The final concentration of DMSO in medium was maximal 1% and DMSO only was used as vehicle control at the same final concentration. Zoledronic acid (Sigma-Aldrich, SML0223) was solved in water (Braun).
Dorsch M., Kowalczyk M., Planque M., Heilmann G., Urban S., Dujardin P., Forster J., Ueffing K., Nothdurft S., Oeck S., Paul A., Liffers S.T., Kaschani F., Kaiser M., Schramm A., Siveke J.T., Winslow M.M., Fendt S.M., Nalbant P, & Grüner B.M. (2021). Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis. Cell Reports, 37(8), 110056.
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6

Cytotoxicity of Lipid Inhibitors and Dox

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For in vitro and in vivo cytotoxic assays with lipid metabolism inhibitors, melanoma cells were treated with 1 μM Lovastatin (Abcam) or 10 μM MF438 (Merck Millipore) in combination with Dox (1 μg/ml) for the indicated time points.
Lehmann J., Caduff N., Krzywińska E., Stierli S., Salas-Bastos A., Loos B., Levesque M.P., Dummer R., Stockmann C., Münz C., Diener J, & Sommer L. (2023). Escape from NK cell tumor surveillance by NGFR-induced lipid remodeling in melanoma. Science Advances, 9(2), eadc8825.
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7

Screening Inhibitors of NP-Induced Endothelial Activation

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To screen for inhibitors of NP-induced endothelial activation, HLMECs were pretreated with 30 μM concentrations of individual chemicals from the microbial natural product library (Target Molecule, Wellesley Hills, MA) for 1 h followed by treatment with 1 μg/ml of N protein for 8 h. The effect of chemicals on endothelial activation were measured by ICAM-1 expression. To evaluate the inhibitory effects of statins on NP-induced endothelial activation, HLMECs were pretreated with atorvastatin, lovastatin, mevastatin, rosuvastatin, or simvastatin (all from BioVision, Milpitas, CA) for 1 h and followed by the treatment of N protein (1 μg/ml) for 8 h. The whole-cell lysates were harvested and analyzed by Western blotting.
Qian Y., Lei T., Patel P.S., Lee C.H., Monaghan-Nichols P., Xin H.B., Qiu J, & Fu M. (2021). Direct Activation of Endothelial Cells by SARS-CoV-2 Nucleocapsid Protein Is Blocked by Simvastatin. Journal of Virology, 95(23), e01396-21.
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8

Screening for NP-Induced Endothelial Inhibitors

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To screen for inhibitors of NP-induced endothelial activation, HLMECs were pretreated by 30 μM/L of individual chemical from microbial natural product library (Target Molecule, Wellesley Hills, MA) for 1 h followed by treatment of 1 μg/ml of N protein for 8 h. The effect of chemicals on endothelial activation were measured by ICAM-1 expression. To evaluate the inhibitory effects of statins on NP-induced endothelial activation, HLMECs were pretreated with atovastatin, lovastatin, mevastatin, rosuvastatin or simvastatin (all from Biovision, Milpitas, CA) for 1 h and followed by the treatment of N protein (1 μg/ml) for 8 h. The whole cell lysates were harvested and analyzed by western blot.
Qian Y., Lei T., Patel P.S., Lee C.H., Monaghan-Nichols P., Xin H.B., Qiu J, & Fu M. (2021). Direct activation of endothelial cells by SARS-CoV-2 nucleocapsid protein is blocked by Simvastatin. bioRxiv.
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